Biology Flashcards

1
Q

Identify the role of enzymes in metabolism, describe their chemical composition and use a simple model to describe their specificity on substrates

A

CHEMICAL COMPOSITION:
Globular proteins; increase reaction rate (catalyse)
Unchanged at end of reaction
Bind to substrate (active site)
Protein molecules (amino acid chain) fold in specific shape
Act on reactant molecule (substrate) fit with at specific location on enzyme molecule surface (active site)

ROLE IN METABOLISM:
Acceleration of chemical reactions
Lowers activation energy needed for reaction; reaction starts quickly without temp change

Lowering of activation energy
Brings specific molecules together (instead of relying on random collisions)

Action on specific substrates
Only one particular enzyme works on one particular substrate molecules
Active site is reciprocally shaped to bind with that molecule

CHARACTERISTICS:
Temperature sensitive
Function best at body temp (above 60℃→ stop working)
Heat breaks hydrogen bonds→ alters active site (not reciprocally shaped)
Temp too high or low→ will denature

pH sensitive
Narrow pH range functions efficiently; levels outside optimum; alters shape

Substrate specific
Each enzyme catalyses one particular reaction; act on one substrate

MODELS:
Induced fit
Enzyme changes shape as substrate approaches (molecules flexible)
Reaction occurs, substrate changes, product released (enzyme returns to original form)
E.g. Gloved hand changes to catch ball; active site is palm, closes around ball when it draws near

Lock and key
Simply fits into active site to form immediate reaction (not considered accurate)
Depends on unlikely random collisions between enzyme and substrate
E.g. Like trying to get key in lock by throwing key at lock with eyes closed

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2
Q

Identify data sources, plan, choose equipment or resources and perform a first hand investigation to test the effect of:

  • Increased temperature
  • Change in pH
A

INCREASED TEMP:
Milk with rennin; curdled quickly (temp approx 370C)
Temps higher or lower than optimum→ milk with rennin doesn’t curdle (doesn’t react)

CHANGE IN pH:
pH affects activity of catalase in potato tissue (has optimum pH)
Height of foam measured when catalase put in hydrogen peroxide
pH of 9 is optimum for catalase (average bubble height was higher)

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3
Q

Identify the pH as a way of describing the acidity of a substance

A

pH scale→ indicates acidity

Lower value; acidic, Higher value ;alkaline

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4
Q

Explain why the maintenance of a constant internal environment is important for optimal metabolic efficiency

A

Stable for enzyme functioning→ maintain metabolism (enzymes sensitive to change)
Small variations from narrow range→ small decreases in activity
Larger variation from narrow range → reduced metabolic efficiency

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5
Q

Gather, process and analyse information from secondary sources and use available evidence to develop a model of a feedback mechanism

A

Stimuli→ increased or decreased body temp (E.g. hot/cold surroundings, exercise)
Co-ordinating centre→ Hypothalamus detects change; activates cooling or warming mechanism
Effectors:
- High temp; Skin vessels dilate (blood carries heat to skin surface) Sweat glands (evaporate)
- Low temp; Skin vessel constrict (reduce heat loss from skin surface) Skeletal muscles (shiver)
Negative feedback loop→ body temp increases or decreases, hypothalamus shuts off warming or cooling mechanism

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6
Q

Describe homeostasis, as the process by which organisms maintain a relatively stable internal environment

A

Maintenance of constant (or almost) internal state, regardless of external environmental change
Body regulates respiratory gas, protects against pathogens, maintain salt/fluid balance, constant temp
Regardless of environmental change→ body temp, blood pH, water/salt balance, blood pressure, oxygen, carbon dioxide concentration; kept constant.

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7
Q

Explain that homoeostasis consists of 2 stages:

  • Detecting changes from the stable state
  • Counteracting changes from the stable stage
A

Any internal deviation must be quickly corrected. Counteract; use corrective mechanism
Stage 1: Detect change from stable state: Receptors detect change. E.g. Thermoreceptors in skin
Stage 2: Counteract change: Effector (muscle or gland) receives message to counteract change. Response initiated to reverse change, restore body to stable. E.g. Muscles shiver to generate heat
If variation exceeds normal; NEGATIVE FEEDBACK counteracts, returns body to homeostasis

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8
Q

Analyse information from secondary sources to describe adaptations and responses that have occurred in Australian organisms to assist temperature regulation

A

GENERATE HEAT:
Shivering: Rapid muscle contractions
Increased metabolism; Activity of thyroid gland stimulated, speeds up metabolism

RETAIN HEAT:
Raised hair: traps warm air, reduces heat loss by convection. Muscles contract
Vasoconstriction: Blood vessels construct so heat carried in blood is redirected to core of body, prevents heat loss from body surface

RELEASE HEAT:
Vasodilation: Arterioles expand, blood directed to body surface, heat lost by radiation, convection
Sweating: liquid secreted onto skin, heat removed to evaporate liquid

GENERATE LESS HEAT:
Decreased metabolism: Thyroid gland lowers metabolism, generates less heat
Flattened hairs: Laid flat, increases heat loss

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9
Q

Outline the role of the nervous system in detecting and responding to environmental changes

A

Function of nervous system→ coordination
Receptors; Thermoreceptors, hypothalamus detects change → converts to message, travels along nerves in CNS (brain, spinal cord)
Control centre: CNS processes info about change in specific parts of brain
Motor nerves; Carry info as nerve impulses from CNS to effectors
Effectors: Muscle or gland receives impulses, instruct effectors to respond
Response; Counteracts original change; ensures homeostasis

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10
Q

Identify the broad range of temperatures over which life is found compared with the narrow limits for individual species

A

Living creatures can survive temps of -70℃ (poles), high as 56℃ (deserts), 350℃ ( hot vents in sea)
Individual species need much narrower range of temp (have optimum temp they function at)
Tolerance range; temp range species can survive, usually few degrees outside of optimum

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11
Q

Compare responses of named Australian ectothermic and endothermic organisms to changes in the ambient temperature and explain how these responses assist temperature regulation

A

Endothermic; maintain constant internal temp; using internal metabolism to generate heat (mammals)
Ectothermic: Body temp governed by external heat sources, environment regulates temp (reptiles)

ENDOTHERMIC: RED KANGAROO
Hottest part of day→ seek shade; tail, hind legs shade by body (reduces surface are exposed to sun)
Lowers body temp

ECTOTHERMIC: BLUE TONGUE LIZARD
Cold weather→ remain inactive (buried in shelter) lowers metabolic rate→ conserve energy
Sunny days→ emerge to bask→ raises temp

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12
Q

Identify some responses of plants to temperature change

A

LEAF FALL:
Hot conditions→ plants drop leaves (reduces surface area to sun, reduces water loss through transpiration)

SHINY LEAVES:
Reflect solar radiation→ reduces heat absorbed

ORIENTATION:
Vertical orientation→ reduces surface area to sun, reduces amount of heat exposed to)

ICE FORMATION BETWEEN CELLS:
Temps below freezing→ ice form in cells, forms in gaps between plant cells; cell walls protects cytoplasm being pierced by ice crystal→ cell survives

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13
Q

PLANTS AND ANIMALS TRANSPORT DISSOLVED NUTRIENTS AND GASES IN A FLUID MEDIUM

A

Transport system; distributes food/oxygen to cells, removes carbon dioxide and waste
Blood; fluid transport medium; contains 3 types of cells
RBC: Carry oxygen, maintain pH of blood
WBC: Part of immune system, protects against invading organism
Platelets: Clotting of blood, stops blood loss
Plasma; Makes up most blood volume; carries nutrients, gases etc

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14
Q

Identify the form(s) in which each of the following is carried in mammalian blood:

  • Carbon dioxide
  • Oxygen - Water
  • Salts - Lipids
  • Nitrogenous wastes
  • Other products of digestion
A

OXYGEN
Carried from lungs to heart, body tissues
98.5% as hemoglobin in RBC, 1.5% dissolved in plasma

CARBON DIOXIDE:
Cellular respiration product carried to lungs
70% as hydrogen carbonate ions, 7% as plasma, 23% combined with haemoglobin
Travels in RBC, plasma

WATER:
Reabsorbed from nephron to body cells
Travels in plasma as water molecules

SALTS:
Reabsorbed from nephrons to all body cells.
Dissolved in plasma as ions

LIPIDS:
Absorbed across villi wall of small intestine to veins in shoulder,
As fatty acids, glycerol dissolved in plasma

NITROGENOUS WASTES :
Urea processed in liver → moves into blood
Transported dissolved in plasma to kidneys (removed across nephrons)

OTHER PRODUCTS OF DIGESTION (AMINO ACIDS, GLUCOSE) :
Proteins broken down into amino acids, transported across small intestine wall.
Dissolved in plasma to be absorbed into cells for making proteins

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15
Q

Perform a first- hand investigation to demonstrate the effect of dissolved carbon dioxide on the pH of water

A

Water in beaker (add universal indicator)
Blow bubbles with straw (carbon dioxide) for 2 mins
Colour will change→ estimate pH using colour chart (makes more acidic)

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16
Q

Perform a first hand investigation using the light microscope and prepared slides to gather information to estimate the size of red and white blood cells and draw scaled diagrams of each

A

Known diameter of RBC= 7.5um
Calculate field of view (mini grid) → on slide estimate number of RBC that fit across diameter of fov
Estimate number of WBC, repeat and compare with known

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17
Q

Explain the adaptive advantage of haemoglobin

A

Haemoglobin: Oxygen carrying molecule (carries 4 oxygen molecules)
Each RBC carried 200-300 million haemoglobin molecules→ so 800-1200 million oxygen molecules
Protein of 4 polypeptide chains (globins) bonded to iron containing group (haem)

INCREASES OXYGEN-CARRYING CAPACITY OF BLOOD:
1 haemoglobin molecule binds with 4 oxygen molecules
More oxygen can be carried in blood cells

INCREASES BINDING OF OXYGEN ONCE FIRST OXYGEN MOLECULE BINDS:
Bonding causes haemoglobin to change slightly, easier for subsequent oxygen molecules to bind
Increases rate and efficiency of oxygen intake

RELEASE OF OXYGEN INCREASED WHEN CARBON DIOXIDE IS PRESENT
Has to release oxygen from blood to where it’s needed
Metabolising cells release carbon dioxide (lowers pH)
Haemoglobin at lower pH has lowered attraction to oxygen (can release)

ENCLOSED IN RBC
If it were just dissolved in plasma, oxygen would upset osmotic plasma balance

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18
Q

Compare the structure of arteries, capillaries and veins in relation to their function

A

Arteries; Carry blood under pressure away from heart to other organs
Capillaries; Tiny blood vessels carry blood close to cells; link arteries and veins
Veins: Carry blood towards heart from other organs

ARTERIES:
Carry blood from heart to other body parts
Thick walls (withstand high pressure of pumped blood)
No valves→ pressure is high (not needed to stop backflow)
Elastic wall fibres→ increases elasticity, expand for increased blood volume pumped in each heartbeat

CAPILLARIES:
Brings blood into contact with tissue (chemical exchange in cells and bloodstream)
Large network to spread blood (no cell far away from blood supply)
Walls only 1 cell layer thick (efficient diffusion)
Small lumen→ Forces RBC to pass in single file (slows flow, increases exposed surface area for gaseous exchange)

VEINS:
Carry blood from tissues back to heart
Thinner walls→ blood flows in, not pumped
Wider lumen (easy blood flow)
Valves (small pocket folds→ lines lumen) → prevents backflow

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19
Q

Analyse information from secondary sources to identify current technologies that allow measurement of oxygen saturation and carbon dioxide concentrations in blood and describe and explain the conditions under which see technologies are used.

A

Levels of chemical in blood→ indicate state of health
Changes in level→ ineffective metabolic functioning (results in poor health)
Carbon dioxide/oxygen concentrations in blood→ how well lungs function, blood circulates

PULSE OXIMETER
Clip with sensor placed on finger→ shows pulse rate and oxygen saturation level
Check blood oxygen levels; people with heart attacks, cancer etc (non-invasive)

ARTERIAL BLOOD GAS ANALYSIS (ABG)
Invasive→ blood removed from artery, blood analysed in sample
Used to discover is patient has lung/kidney disorder, lung disease
Details about level of chemicals in blood (pH, bicarbonate ions, oxygen levels)

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20
Q

Describe the main changes in the chemical composition of the blood as it moves around the body and identify tissues in which these changes occur.

A

Circulatory system; transport of substances to and away from parts (gases, nutrients, wastes, hormones)
Metabolism→ relies in correct chemical balance brought to cells, removal of wastes
Function of organ→ determines difference in chemical concentration of blood entering or leaving
All organs→ Internal gae exchange (cellular respiration) lungs→ external
Deoxygenated blood→ arrives at lungs, releases CO2 and picks up oxygen→ Haemoglobin carries
CO2→ cells release,diffuses into capillaries→ carried in haemoglobin→ travels back via veins

BLOOD PASSING THROUGH LUNGS:
Increase Oxygen, & Decrease CO2

BLOOD PASSING THROUGH ANY ORGAN NOT LUNGS:
Decrease Oxygen & Increase CO2

BLOOD PASSING THROUGH ANY ORGAN INVOLVING ABSORBING DIGESTED FOOD:
Increase in digestive end products (glucose)

BLOOD PASSING THROUGH LIVER:
Decrease in digestive end products (E.g. Glucose, fatty acids, amino acids)
Increase nitrogenous wastes (Urea)

BLOOD PASSING THROUGH KIDNEY:
Decrease nitrogenous wastes (filter and excrete)

BLOOD PASSING THROUGH GLANDS:
Increase in hormones (secreted and travel to where needed)

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21
Q

Outline the need for oxygen in living cells and explain why removal of carbon dioxide from cells is essential

A

Oxygen→ necessary for cellular respiration (combines with glucose during CR to release energy ATP)
CO2→ Must be removed to prevent pH changes in cells and bloodstream
CO2 reacts with water (in cytoplasm or plasma) → forms carbonic acid (build up is toxic) lowers pH
Lowered pH→ prevents enzyme functioning (reduces metabolic efficiency)

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22
Q

Analyse information from secondary sources to identify the products extracted from donated blood and discuss the uses of these products

A

First transfusions (most killed) 120 years ago → discover specific blood types (incompatible groups= fatal)
Before blood donations→ cross matching of blood groups needed
RBC→ helps patients carry more oxygen (helps replace lost cells after bleeding)
Platelet→ treats bleeding from diseases where platelets don’t function properly
Frozen plasma→ patients who need immediate clotting (E.g. After large transfusions

LIABLE PRODUCTS:
Perishable→ short shelf life
Need to be transported in refrigerated conditions
E.g. RBC, platelets, plasma

STABLE PRODUCTS:
Longer shelf life
Produced by- separating different protein components from plasma
E.g. Blood clotting factors, immunoglobulins

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23
Q

Describe current theories about processes responsible for the movement of materials through plants in xylem and phloem

A

XYLEM:
Carries water ions from roots to leaves
Made of vessels, tracheids, fibres, parenchyma cells

Transpiration stream theory
Water sucked up stem; evaporative pull of transpiration
Water drawn up tubes; replace water loss from evaporation in leaves
Evidence:
Vessels are hollow→ offer little resistance to water
Concentration gradient; leaf surface (high), centre of leaf (low) creates tension as moves across gradient→ doesn’t break due to cohesion/adhesion of molecules

PHLOEM:
Carries nutrients (sugars, amino acids) to all parts of plant, moves both ways
Made of fibres, parenchyma, sieve cells and companion cells

Pressure flow theory
Active process (needs energy) driven by osmotic pressure gradients (generated by differences in sugar water concentration)
Sugar loaded into phloem at source then uploads into surrounding tissue (sink)
Loading attracts water flow (osmotic pressure)
Offloading at sink→ water moves out

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24
Q

Analyse and present information from secondary sources to report on progress in the production of artificial blood and use available evidence to propose reasons why such research is needed

A

Past→ attempts to treat bleeding in WW1 & WW2→ failed. Encouraged modern artificial blood
Blood transfusions work (Problems; need cross matching and short storage life)
1980’s→ urgent research; response to sudden appearence of HIV in blood transfusion patients
AIDS crisis in South Africa- driving force in becoming one of the first countries to clear artificial blood for limited use in patients

IDEAL CHARACTERISTICS:
Can be stored for long periods of time and easily transported
Doesn’t need to be cross matched for different blood types
Continues to circulate (doesn’t settle) and has no toxic effects on body
When patient’s own blood is restored→ can be safely excreted

Oxygen carriers being developed: Perfluorocarbons, haemoglobin based oxygen carriers and microcapsules

PERFLUROCARBONS:
Carry oxygen in dissolved forms
Carry up to 50x more dissolved oxygen than plasma

HAEMOGLOBIN BASED OXYGEN CARRIERS:
Extract haemoglobin from outdated human blood; modify for use in artificial blood

MICROCAPSULES:
Artificial red cell currently being developed as microcapsules
Phospholipid- haemoglobin can be placed inside

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25
Q

Explain why the concentration of water in cells should be maintained within a narrow range for optimal function

A

Changes in water concentration lead to corresponding changes in solute concentration in cells
Water in cells→ determines osmotic pressure of cells
Water moves by osmosis→ water movement into/ out of cells depends on solute concentration inside/ out of cells
Water provides necessary medium in which all chemical reactions of metabolism occur:
Chemical reactions occur→ only if reactants are dissolved in water; levels must be constant
Water concentration; Too much→ cells may burst. Too little→ cell contents shrink
Too little water→ increase in solute concentration→ lowers pH (must be maintained→ enzymes)
Water accumulates→ may dilute reactants and slow down metabolism

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26
Q

Explain why the removal of wastes is essential for continued metabolic activity

A

Accumulation of wastes is toxic→ must be removed to maintain homeostasis
If build up→ alters conditions→ stops enzyme functioning. Can change pH→ stops enzymes
Accumulation that doesn’t alter pH→ alters reaction rates, osmotic imbalance→ affects membrane functioning
E.g. Accumulation of Carbon Dioxide→ internal environment becomes too acidic

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27
Q

Gather, process and analyse information from secondary sources to compare the process of renal dialysis with the function of the kidney

A

KIDNEY:
Filters blood, remove waste/excess fluids→ turn into urine (excreted)
Passive: Glomerular capillaries diffuse wastes through membrane
Active: Reabsorption in nephrons, wastes reused into bloodstream
180 L blood filters everyday (entire blood volume filtered 20-25 x per day)
Maintains chemical balance in blood

RENAL DIALYSIS:
Carries out function of failed kidneys (cleans blood)
Haemodialysis→ transfers blood to machine to be filtered before returned to body
Glucose levels same in fluid (so doesn’t diffuse)
Passive transport; diffusion of substances in dialysis membrane between blood and fluid
Blood filtered for 3-4 hours (2-3 times a week)
Removes wastes to stop accumulating; maintains chemical balance in blood

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28
Q

Identify the role of the kidney in the excretory systems of fish and mammals

A

Water accumulates in body→ by eating/drinking, metabolism. Nitrogenous wastes→ metabolism
Water potential: Tendency of a solution to lose water by osmosis (typical; high water concentration)
Water concentration in environment; Determines organism’s need to conserve or lose water

FRESHWATER FISH:
Live in rivers/lakes (high water potential) water freely available (few salts)
Urinate frequently; water accumulates (osmosis→ high concentration surroundings to low in fish)
Too much water in bodies→ Kidneys excrete excess and wastes. Conserve salt

MARINE FISH:
Live in sea- urinate less (lose body water across gills to surroundings)
Salt diffuses into bodies→ main kidney function (remove excess)
Kidneys conserve water rather than extract

TERRESTRIAL MAMMAL:
Water and salt loss from body;respiration ,excretion (sweat, urine)
Control mechanism; ensure balance maintained of amount excreted
Urine; dilute or concentrated (adjusted depending on body needs)
Large amount of salt lost by sweat→ needs replacing for stable osmotic pressure in body

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29
Q

Explain why the process of diffusion and osmosis are inadequate in removing dissolved nitrogenous wastes in some organisms.

A

Both are slow (rely on concentration gradient difference; slows as difference is smaller, stops; equal)
Solution; combine active transport and osmosis (quick; removes waste even against gradient)
Used to pump salts from urine back into kidney (draws water with them by osmosis)

PROBLEMS WITH DIFFUSION:
Rate of movement is slow
Wastes must be dissolved with water when removed→ Concentrations equalise movement slows and stops
Not all wastes can be removed by diffusion
If concentrations equalise and no further wastes removed→ pH would be altered

PROBLEMS WITH OSMOSIS:
Too much water may be lost in urine
Contains too much nitrogenous wastes, water will be drawn into urine to dilute waste (equalise concentration) → dilute urine (loses too much)
Movement of water may make wastes too dilute for excretion by diffusion
Slows down excretion by diffusion (lowers concentration gradient)

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30
Q

Present information to outline the general use of hormone replacement therapy in people who cannot secrete aldosterone

A
Adrenocortical insufficiency (Addison’s disease)  some immune systems cause inflammation in own glands, other causes are tuberculosis and cancer
Decreased secretion of aldosterone→  increased sodium loss
Clinically; Person is weak, thin, have salt-cravings, faintness, feel light-headed, low blood-sugar 
Most patients → require treatment with fludrocortisone plus cortisone as a replacement glucocorticoid
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31
Q

Distinguish between active and passive transport and relate these to processes occurring in the mammalian kidney

A

Passive→ diffusion, osmosis (molecules move along concentration gradient) no energy input
Active→ needs cellular energy to move molecules against concentration gradient
Diffusion; Particles from region of high concentration to low until equilibrium is reached
Osmosis; water molecules from high water concentration to low,through semi permeable membrane

PASSIVE TRANSPORT IN KIDNEY:
Limitations; relies on difference in concentration gradient (slow)
Tubules→ wastes from bloodstream to be excreted as urine
Substances needed→ removed from urine and returned to bloodstream
Passive moves water (osmosis), some wastes (ammonia, urea) into kidney

ACTIVE TRANSPORT IN KIDNEY:
Sometimes have to move against gradient
Carrier proteins spans membrane and carrier molecule actively move chemicals from low to high concentration using cellular energy
Mainly sodium ions, glucose, amino acids across wall of nephron (reabsorbed)
Sodium pump in tubules→ transports salts from urine back into kidney (conserve salt and water→ salt draws water)

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32
Q

Analyse information from secondary sources to compare and explain the differences in urine concentration of terrestrial mammals, marine fish and freshwater fish.

A

Urine concentration; depends on need to conserve water.
High solute; concentrated. Low solute; dilute

FRESHWATER FISH:
Only dilute
Sources; drinks, osmosis into fish

MARINE FISH:
Only concentrated
Sources; Drinks,osmosis out of fish

TERRESTRIAL MAMMAL:
Conserve water (concentrated)
Consumes water (dilute
Source; Drinking, eating

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33
Q

Explain how the processes of filtration and reabsorption in the mammalian nephron regulate body fluid composition

A

FILTRATION:
Occurs between glomerulus, lining of Bowman’s capsule
High pressure; blood flowing through glomerulus (small substances squeeze through capillary wall under pressure→ pass through cell layer in Bowman’s capsule→ move into lumen)
Pass through; blood cells, proteins, water→ carry dissolved amino acids, glucose, salts, wastes (fluid; glomerular filtrate)
Substances body needs; reabsorbed into bloodstream (so not lost with urine)

REABSORPTION:
Filtrate with molecules needed; amino acids, glucose→ actively reabsorbed into proximal tubule
Passed to interstitial fluid, capillaries surrounding nephron→ to renal vein; carried back to general circulation
99% water reabsorbed by osmosis, only 1% excreted as urine
Ascending loop→ salts actively pumped into interstitial fluid in medulla (draw water out by osmosis)

SECRETION:
Toxic substances removed from capillaries, tissues
Drugs secreted in proximal tubule, urea in descending loop of Henle

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34
Q

Use available evidence to explain the relationship between the conservation of water and the production and excretion of concentrated nitrogenous wastes in a range of Australian insects and terrestrial mammals

A

Limited water availability→ must conserve water and excrete waste
High water availability→ water conservation not necessary (waste may be dilute)
Moth, blowfly→ uric acid as paste (low toxic) high energy needed, but conserve water
Spinifex hopping mouse→ concentrated urea; conserve water (moderate toxic)
Humans, moth, blowfly→ water not freely available (must be sourced)

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35
Q

Outline the role of the hormones, aldosterone and ADH (anti- diuretic hormone) in the regulation of water and salt levels in blood

A

ADH
Dehydration, blood volume drops→ detected by hypothalamus in brain; stimulates pituitary gland to release ADH→ acts on nephrons to increase reabsorption of water
Increases permeability of membranes lining distal, collecting tubule; water reabsorbed (conserved)

ALDOSTERONE
Decrease in sodium ion concentration in blood→ secreted from adrenal gland cortex (above kidney)
Aldosterone via bloodstream reaches kidney; increase permeability of nephron to sodium→ reabsorption into surrounding kidney tissue (less lost by urine)

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36
Q

Process and analyse information from secondary sources and use available evidence to discuss processes used by different plants for salt regulation in saline environments.

A

MANGROVE:
Concentrate salt accumulation to certain parts (leaf) → fall off, salt leaves mangrove.
Leaves; salt glands secrete salt entering; can be blown or washed away
Roots; first line of defense→ filter out incoming salt

BLADDER SALTBUSH:
alt enters roots, travels to leaves. Stored in vacuole and moves to bladder cell
Bladder cell ruptures→ salt released

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37
Q

Define enantiostasis as the maintenance of metabolic and physiological functions in response to variations in the environment and discuss its importance to estuarine organisms in maintaining appropriate salt concentration

A

Enantiostasis:
Survival mechanism; organisms cope with extreme fluctuations in environmental conditions
Estuary:
Water/salt concentrations fluctuate daily→ high tide; salt in river. Low tide; freshwater in
Organisms need to maintain normal metabolic functioning (despite fluctuations)

OSMOCONFORMERS:
Tolerate change; alter internal solute concentration to match external environment
E.g. Fiddler crab accumulates additional solutes in high salt, then pumps out excess when low salt

OSMOREGULATORS:
Avoid change in internal environment; keep solutes at optimal level regardless of environment
E.g. Mussels in rock pools close valves when tide it out (keeps salt concentrate same as seawater)

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38
Q

Perform a first-hand investigation to gather information about structures in plants that assist in the conservation of water.

A

SHEOAKS:
Needle like leaves→ reduces surface area, reduces water loss

EUCALYPTUS:
Waxy cuticle→ reflects sun, reduces water loss by evaporation
Leaves hang vertically→ reduces exposure to sun

GREVILLEA:
Small curled leaves→ Retain more water
Hairy leaves→ hair returns water, increases humidity

WATTLE:
Grey colour→ Light colour to reflect sunlight, reduces evaporation
Hair on undersurface→ retains water

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39
Q

Describe adaptations of a range of terrestrial Australian plants that assist in minimising water loss.

A

Needle-like leaves→ reduces surface area, water loss. E.g.She-oakes
Woody fruits→ Less water loss that in fleshy fruits. E.g She-oakes
Leaf curling→ Reduce surface area. Traps humid layer of air→ reduced water loss.
Hanging leaves→ reduce exposure to sun
Hairy/shiny leaves→ hairy undersurface→ reduces air movement, increase humidity→ less water loss. Upper surface, reflects radiation from sun (reduced heat gain)
Water directing leaves/stems→ shaped so water runs down to roots. E.g. Acacia

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40
Q

EVIDENCE OF EVOLUTION SUGGESTS THAT THE MECHANISMS OF INHERITANCE, ACCOMPANIED BY SELECTION, ALLOW CHANGE OVER MANY GENERATIONS

A

Macro evolution→ Millions of years, arising new species. E.g wolf and dog from common ancestor
Micro evolution→Shorter time periods, pop changes but no new species. E.g. Different dog breeds

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41
Q

Outline the impact on the evolution of plants and animals of

  • Changes in physical conditions in environment
  • Changes in chemical conditions in environment
  • Competition for resources
A
  • Changes in physical conditions in environment
    Early organisms; water to land habitat→ reduced UV radiation (ozone forming)
    Aus climate; cool/wet→ hot/dry, rain forests to woodland,
    Lakes dry up→ evolution to conserve water
    Ice age→ change in sea levels, temp. Dinosaurs→ meteorite; reduced light, plant life→ no food
  • Changes in chemical conditions in environment
    First life; anoxic environment; some produced CO2; led to photosynthetic organisms
    Increased oxygen levels; evolution of organisms using oxygen (complex-diverse animals today)
    E.g. Peppered moth; industrial revolution. Black moth protected from soot; white stand out and killed
  • Competition for resources
    Comp for light, soil, nutrients, water, shelter, mates, territory
    Organisms compete; most successful survive and reproduce; pass on genes
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42
Q

Plan, choose equipment or resources and perform a first-hand investigation to model natural selection

A

Pop begins with 30 moths (10 black, grey, white) → chart works out offspring colours.
Spin for predator (colour removed) shuffle cards; repeat until trend recognisable; dominant species?

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43
Q

Analyse information from secondary sources to prepare a case study to show how an environmental change can lead to changes in species (SNOW GUM)

A
HIGH ALTITUDE:
Cold, shallow soil, exposed to snow
Small and twisted to bend away from elements. 
Short leaves
Large fruit
Thin bark 
More resistance to frost
Short trees 
LOW ALTITUDE: 
Warm, high precipitation,
Tall and straight to receive nutrients and rainfall 
Long leaves
Small fruit 
Thick bark 
Less resistance to frost
Tall trees
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44
Q

Describe, using specific examples, how the theory of evolution is supported by the following areas of study:

  • Palaeontology, including fossils that have been considered as transitional forms,
  • Biogeography,
  • Comparative embryology,
  • Comparative anatomy,
  • Biochemistry
A

PALAEONTOLOGY:
Scientific study of fossils and extinct life
Fossils→ evidence of past life forms; evolutionary transitions to modern living forms
Undisturbed rock fossils; show sequence living things arose; have common features (change over time)
E.g. Lobe- finned fish; bones in fin→ dragging from water to land (amphibians evolved from fish)
Limitations; fossil record incomplete, bias to fossils with body/environment better suited to fossilisation

BIOGEOGRAPHY:
Study of geographical distribution of organisms
Darwin/Wallace theory; new species; group isolated from rest→ thought species close; similar, far apart; different
E.g. Flightless birds/continental drift→ common Gondwana ancestor; different pop evolved on continents. E.g. Emu in Aus, Kiwi in NZ→ share similar features; flat breastbone

COMPARATIVE EMBRYOLOGY:
Comparison of similarities in vertebrate early embryos
Embryos of closely related organisms have homologous parts→ support common ancestor
E.g. Fish, bird, mammal, reptile embryos; gill slits, tails (later internal gills in fish)

COMPARATIVE ANATOMY:
Similarities in organisms structure (similarities; common ancestor, differences; modification) evolution
Limitations; fossils often incomplete→ hard to compare with extinct life form
E.g. Pentadactyl limb; (homologous structure) same basic sequence of bones in dog, human, bird;

BIOCHEMISTRY:
DNA hybridisation:
Compare DNA sequence of 2 organisms; unzip, zip codes to match
E.g. Heat applied to chimpanzee, human DNA→ high temp means more closely related; 83℃
Amino acid sequencing
Similarities in protein sequencing→ Similarities; shared ancestor. Differences; evolved over time
E.g. Humans. chimps→ identical sequence in haemoglobin. More related than gibbons,humans (3 differences)

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45
Q

Use available evidence to analyse using a named example, how advances in technology have changed scientific thinking about evolutionary relationships (CLASSIFICATION OF PRIMATES CHANGED)

A

DNA in amino acid sequencing, DNA hybridisation→ new biochemical evidence
Historically; orangutans, gorillas, chimps→ 1 family, humans another (based on structure of leg, teeth)
60’s→ Chimps, humans→ identical haemoglobin, cytochrome c sequence→ different to gorilla
Humans, chimps small DNA difference, (more closely related than orangutans→ diverged earlier)
New genetic tree→ humans, chimps diverged more recently from common ancestor

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46
Q

Explain how Darwin/Wallace’s theory of evolution by natural selection and isolation accounts for divergent evolution and convergent evolution

A

Proposed; variations within species and more offspring produced than can survive and reproduce
Some individuals have adaptive characteristics; enable survival better→ passed on to next generation
Over time; natural selection→ pop with adaptations most suited to environment

Source of variation; gene mutation; phenotypic advantage
Isolation; if species pop geographically isolated, interbreeding stops; separate species develop
Divergent; one species forms other with adaptations suited to variety of environments
E.g. Aus marsupials; evolved from common possum like ancestor; common structure, but dominant differences
Convergent; Organisms come to resemble each other; share similar environment, perform same function. E.g. Streamline dolphin/shark body for swimming in sea. Similar but dolphin; mammal, shark; fish

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47
Q

Analyse information from secondary sources on the historical development of theories of evolution and use available evidence to assess social and political influences on these developments

A

England 1858→ Darwin/Wallace published theory
Invention of machinery, people flocked to cities (disease) social changes in class, French revolution
New discoveries; people looked to science.

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48
Q

Outline the experiments carried out by Gregor Mendel

A
Heredity in garden peas; pure bred (consistent characteristics) 
Deliberately crossed one variety with another→ observed next generation
Removed stamens (so no self pollination) repeated experiments, kept records
Monohybrid cross; Offspring of cross (F1) Crossbred tall x short (all offspring tall) Tall then grew (F2) F2 most tall, some short (3:1)
Law of segregation; 2 genes that control each characteristic; segregate during reproduction; 1 factor each in a gamete→ factors recombine at fertilisation (match together)
Law of Independent assortment; Pairs segregate independently of other pairsof factors
Reproductive cells combine at fertilisation; offspring had one factor for tallness and one for shortness→ only tallness observed (dominated shortness)
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49
Q

Describe the aspects of the experimental techniques used by Mendel that led to his success

A

Cross pollinated by hand, studied large number of characteristics
Used quantitative data, studied characteristics one at a time
By chance→ characteristics he studied carried out on different chromosome
Studied separately characterises occurring in pairs (tall or short) previously; whole plant studied

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50
Q

Solve problems involving monohybrid crosses using Punnett squares or other appropriate techniques

A

Check for dominance and assign symbols
Write down parents phenotype and genotype
Write down parents gametes gametes, noting only one allele for characteristic in gamete
Make punnett square and write down all possible crosses underneath

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51
Q

Describe outcomes of monohybrid crosses involving simple dominance using Mendel’s explanations

A

2 different parents→ F1 generation only has dominant trait

F1 crossed→ F2 generation has dominant trait, recessive trait in (3:1)

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52
Q

Process information from secondary sources to describe an example of hybridisation within a species and explain the purpose of this hybridisation

A

Hybridisation; Crossbreeding two genetically non-identical individuals
Parents with desirable traits selected; offspring reflecting desired traits further breed; hybrid offspring
E.g. Hybridisation within species: Labradoodle (Labrador x Poodle) → successful hybridisation leads to hybrid vigour (increased strength, better health, greater fertility)

ADVANTAGES:
Increases genetic variety
Combine best features of each parent→ hybrid vigour

DISADVANTAGES:
May combine weaker features of parents→ offspring have less stamina, resistance to disease etc
Very expensive (especially if no hybrid vigour)
Sometimes offspring are infertile or reduced fertility

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53
Q

Distinguish between homozygous and heterozygous genotypes in monohybrid crosses

A

Homozygous→ Identical alleles of a particular gene for a characteristic.
E.g. TT, tt, HH, hh

Heterozygous→ Two different alleles of a particular gene for a characteristic.
E.g. Aa, Bb, Ee

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54
Q

Distinguish between the terms allele and gene, using examples

A

Gene→ Smallest unit of hereditary. Codes for a particular characteristic
E.g. Eye colour gene

Allele→ Variations of a gene
E.g. Brown, blue, green, black eye colour

55
Q

Explain the relationships between dominant and recessive alleles and genotype using examples

A

Dominant alleles→ Form of gene expressed in heterozygous condition, masking the other (recessive) form of same gene. Written in UPPERCASE letters.
E.g. T

Recessive alleles→ Form of gene expressed in homozygous condition. Written in lowercase letters.
E.g. t

56
Q

Outline the reasons why the importance of Mendel’s work was not recognised until some time after it was published.

A

Paper only presented to small group; work suddenly appeared (may not have been noticed)
Work radically different to previous; may not have been understood and little known about cels
He wasn’t recognised scientist; more likely to have been noticed if was serious scientist

57
Q

Outline the roles of Sutton and Boveri in identifying the importance of chromosomes

A

SUTTON: (1877-1916)

Meiosis of grasshopper cells→ observed chromosomes occur in visible pairs in meiosis (paternal and maternal)
During meiosis; chromosome number is halved, chromosome in each pair separate; gamete receives one chromosome from each pair→ fertilisation resotes full number in zygote
Concluded that:
Chromosomes carriers of hereditary units;arrange themselves independently in middle of cell before divides
Chromosomes units involved in inheritance; believed that several Mendelian factors present in one chromosomes; therefore inherited as unit

BOVERI (1896-1904)

Sea urchin egg experiment→ behaviour of cell nucleus and chromosomes (in meiosis and fertilisation)
Already known→ organisms has set number of chromosomes and in fertilisation (egg and sperm fuse)
Found nucleus of egg and sperm contribute 50% of chromosomes to zygote

Experiments:
Egg and sperm fuse; resulting offspring have characteristics of parents. If only one parent nucleus; abnormalities→ showed that complete set of chromosomes needed for normal; inheritance factors on chromosomes in nucleus

Before→ Hereditary factors found in nucleus and cytoplasm; After→ Nucleus only
Before→ Proteins store hereditary info. After→ full set of paired chromosomes
Before→ gametes transport factors to pass onto next generation. After→ random assortment during meiosis
Before→ Chromosomes believed to disappear and reappear. After→ Occur in set numbers, in pairs

58
Q

Describe the chemical nature of chromosomes and genes

A

Chromosome→ made of DNA, proteins
Proteins→ histone proteins bind to DNA to form chromatin (in nucleus)
DNA→ Double helix structure, basic building block of nucleotide (base, sugar, phosphate)
4 bases; Adenine (A) and Thymine (T), Cytosine (C ) and Guanine (G)

Diagram of Nucleotide
2 nucleotides linked by covalent bonds between sugar and phosphate
DNA tightly wrapped around histone→ protect from damage, allow long DNA length to be packaged so it doesn’t move around cell in cell division

59
Q

Identify that DNA is a double-stranded molecule twisted into a helix with each strand comprised of a sugar-phosphate backbone and attached bases- adenine (A), thymine(T), cytosine ( C) and guanine (G)- connected to a complementary strand by pairing the bases A-T and G-C

A

DNA contains info in specific sequence of nucleotides (sugar, phosphate, base)
Spiral double helix (rungs of ladder→ hydrogen bonds linking (A,T) and (C,G)

60
Q

Identify data sources and perform a first hand investigation to demonstrate the effect of environment on phenotype

A

Radishes grown in 2 petri dishes; one kept in light. Other kept in dark
In dark: grew taller faster; but had yellow leaves → In light: shorter height
Radishes in dark moved into light→ leaves became green; gene undamaged, but not expressed in dark

61
Q

Explain the relationship between the structure and behaviour of chromosomes during meiosis and the inheritance of genes

A

In meiosis; genetic variation from crossing over, random segregation, independent assortment
Meiosis; One cell (2 divisions) → 4 haploid cells (genes in each; new combo of parent genes)
Independent assortment; paternal and maternal chromosomes sort out independently of one another
Maternal don’t all move into one gamete and paternal into another→ random where it ends up→ mixes paternal and maternal (genetic variation)
Chromosomes in pair separate→ ensures chromosomes number in gametes is half of original

62
Q

Explain the role of gamete formation and sexual reproduction in variability of offspring

A

Variability→ Different forms of a gene within a population (from genes, environment or both)
Sexual reproduction increases variability→ increasing gene recombination
Greater variability improves pop’s ability to adapt to changes in environment
Meiosis and fertilisation→ when gametes form, cross over, random segregation→ results in genetic recombination of paternal and maternal genes within each gamete

63
Q

Describe the inheritance of sex-linked genes, and alleles that exhibit co-dominance and explain why these do not produce simple Mendelian ratios

A

Mendelian ratios→ only when similar conditions to Mendel; if genes don’t assort independently,or show dependence→ Mendel’s ratios not obtained

Cells; 23 chromosome pairs (22 x autosomes, 1x sex chromosomes) Female genotype XX, Male; XY
Offspring;equal chance of male or female; determined during meiosis; transfer 1 sex chromosome to each gamete; fusion of gametes in fertilisation

Meiosis; Females (44 autosomes plus XX) → halved egg gets 22 autosomes and X. Males→ Half get 22 autosomes and X, half get 22 autosomes and Y
X from mother and father→ will be female (XX) X from mum, Y from dad→ will be male (XY)

Mendel’s experiments; no sex-specific effects; sex-linked inheritance is deviation from Mendel’s ratios

64
Q

Describe the work of Morgan that led to to the understanding of sex linkage

A

At time; Unknown why number of traits that separate in meiosis exceeds number of chromosomes
Sutton/Boveri→ more than one trait is present on each chromosomes (didn’t demonstrate)
1910→ Morgan experiments on gene eye colour in fruit flies; located on X chromosome→ heredity factors exchanged between X chromosomes
Drosophila Melanogaster (normally red eyes, but found mutant white eyed male) → crossed to see if white eyed gene would show Mendel ratio

CROSS 1:
Pure bred parents o obtain F1 hybrid offspring
White eyed male and red eyed female

CROSS 2:
Crossed F1 hybrid offspring to obtain F2 generation
Expecting Mendelian 3:1 ratio but more than 80% had red eyes and less than 20% white eyes
Most white eyed were male. Thought females couldn’t have white eyes

CROSS 3:
Typical cross to investigate hypothesis → crossed white eyed male with heterozygous red eyed female
Results shown in F2 generation,both males and females had white eyes
Next (and correct) hypothesis→ white eyed characteristic is ‘sex-limited’. Genetic crosses proved red eyes were sex limited (carried on sex chromosome)

65
Q

Explain the relationship between homozygous and heterozygous genotypes and the resulting phenotypes in examples of codominance

A

Doesn’t show Mendelian pattern (pairs of alleles don’t show dominance over the other)
Heterozygote where 2 different alleles for same gene; both expressed separate (both dominant)
E.g. Cattle have red or white→ Heterozygous may have red and white coat→ roan (not in patches but interspaced red and white hairs)

66
Q

Outline ways in which the environment may affect the expression of a gene in an individual

A

Genotype + environment= phenotype
E.g. (TT)plant may end up same size as (tt), if grew in unfavourable conditions (lack of water)
E.g. Temp can affect phenotype of animals: or effect of soil pH on colour of hydrangea flowers;
Pink flowers in alkaline or blue flowers in acidic
Identical twin; differences due to environmental rather than genetics→ One may have freckles (sun exposure) other may not
Environmental factors influencing humans; smoking, physical activity levels, diet, nutrition

67
Q

Describe the process of DNA replication and explain its significance

A

DNA replication→ DNA copied; produce new molecules with same base sequence
STAGE 1: DNA double helix unwound, separated into strands (hydrogen bonds broken)
STAGE 2: Strands act as templates→ Nucleotides and bases form bonds with bases on parent strand
STAGE 3: Daughter DNA molecules rewind in double helix; identical in base sequence to parent (due to complementary base pairing)
Each of new strands is complementary to template it was made on and identical to other template

68
Q

Outline using a simple model, the process by which DNA controls the production of polypeptides

A

Polypeptide synthesis; uses RNA→ single stranded nucleic acid (bases A, C, G and Uracil→ replaces Thymine; T)
Three types of RNA: mRNA (messenger RNA) tRNA (Transfer RNA) and rRNA (ribosomal RNA)
DNA to polypeptide; DNA code determines amino acid sequencing in polypeptide chain→ DNA info transcribed to mRNA from template strand
3 nucleotides on mRNA→ codon for particular amino acid

TRANSCRIPTION STAGE:
Copy of gene is made (RNA not DNA) → carries info for making polypeptide (mRNA)
Double helix unwinds; RNA nucleotides assemble use one DNA strand as template; mRNA separates from DNA and double helix reforms

TRANSLATION STAGE:
Ribosomes move along mRNA molecule→ transfer RNA molecules present around ribosomes (bind to them→ only bind to anticodon that is complementary to codon on mRNA)
Codon and anticodon base link (form hydrogen bonds)
tRNA molecules carry amino acids→ bonded by peptide linking; dipeptide formed→ upper tRNA detaches, another one binds→ chain of 3 amino acids forms
These stages are repeated until a polypeptide is formed

69
Q

Analyse information from secondary sources to outline the evidence that led to Beadle and Tatum’s ‘one gene- one protein’ hypothesis and to explain why this was altered to the ‘one gene- one polypeptide’ hypothesis

A

1941→ mutants of fungus→ led to discovery of genes provide instructions for making proteins
Hypothesis; gene controls production of one enzyme→ evidence needed
Designed experiment to attempt to mutate genes of mould→ evidence led to hypotheses; genes affect enzyme productivity→ Breakthrough! (At time; ongoing debate; heredity material protein or DNA?)
EXPERIMENT:
Irradiated bread mould with X-rays (induce mutations) → showed some mutants no longer produced amino acid (implies particular enzyme no longer functions)
Tested; If loss of function had genetic basis→ crossed mutant with normal mould; found offspring shared mutant phenotype→ inability to produce amino acid is inherited (due to mutation)
Found different enzymes in different mutants altered or missing; proved gene determines structure of enzyme→ proposed one gene-one enzyme hypothesis
Changed to one-gene-one protein when demonstrated other proteins besides enzymes encoded by gene
Found one gene not necessarily responsible for structure of entire protein; but for each polypeptide chain making up protein (currently accepted)

70
Q

Explain the relationship between proteins and polypeptides

A

Proteins: Amino acid chain joined by peptide bonds; may be from many polypeptide subunits
Polypeptide: Molecule consisting of many joined amino acids→ aa joined by peptide bonds
To make particular polypeptide, amino acids must be linked up in a precise sequence
Genes store info needed for making polypeptides→ info is stored in coded form
Precise sequence of bases in gene codes for the sequence of amino acids in a polypeptide

71
Q

Explain how mutations on DNA may lead to the generation of new alleles

A

Mutagens are environmental agents that cause mutations;
Chemical (asbestos), Biological (HIV), Mutagenic radiation (UV, nuclear reaction)
Changes to genetic material arise during DNA replication
Mutations alter genes; change nucleotide sequence in DNA→ One or more genes may be altered from original form (Allele 1, Allele 2) 2 variations of gene is formed→ new allele formed
One or more genes may be altered from original. E.g, Allele 1→ altered polypeptide→ new protein
Most new proteins have little effect on organism, but some lead to genetic disorders, inherited diseases
Effect of new proteins on next generation:
If mutation in a somatic cell→ individual may be affected but no effect on future generations
If mutation is in germline cell (gametic mutation) → alleles can be inherited and have significant effects on population→ result in evolution
Changes to genetic material arise during DNA replication→ result in Change to single gene (gene mutation) or rearrangement of blocks of whole chromosomes (chromosomal mutations)

72
Q

Discuss evidence for the mutagenic nature of radiation

A

During late 1800’s and early 1900’s, many scientists were involved in studying radiation
Harmful effects of radiation were unknown→ scientists such as Marie Curie who were exposed to large amounts of radiation over a prolonged periods of time, developed various illnesses
Marie Curie worked with ionising radiation for most of career; died from leukemia (overexposure)
Survivors of Hiroshima bomb→ suffer physical mutations (radioactive output of nuclear explosion)

73
Q

Explain how an understanding of the source of variation in organisms has provided support for Darwin’s theory of evolution by natural selection

A

Mutation; basic source of variation
Mutations affect base sequence of DNA→ understand how can be passed from one generation to next→ supports Darwin’s theory of evolution (Explains how heritable variation arises)
Mutation will result in change in phenotype, may not do anything, or may confer advantages disadvantage to organism
Mutations provide diversity of genetic material that results in variation in phenotype
If mutations can be inherited→ provide variation on which natural selection acts
Evolutionary purposes, mutation can be redefined as heritable change in genetic material

74
Q

Process and analyse information from secondary sources to explain a modern example of natural selection

A

Majority of mutations; detrimental to or have no significant effect on environment; some beneficial

INSECTICIDE RESISTANCE:
Some organisms→ developed resistance; most killed, some naturally resistant survive and reproduce (pass genes→ resistant pop develops)
E.g. Sheep blowfly lay eggs in wet wool; burrow into skin→ loss of production → developing resistant to agricultural insecticides
If insecticides rotate, effectiveness increased

ANTIBIOTIC RESISTANCE:
Many bacterial infections treated with antibiotics. Interfere with protein synthesis
Many bacteria become resistant to wide range of antibiotics
Animals given antibiotics to grow faster and not have disease→ if humans eat or drink milk from animals, then resistant bacteria could be transferred to humans
Encouragement of doctors to restrict general use of some selected antibiotics

75
Q

Describe the concept of punctuated equilibrium in evolution and how it differs from the gradual process proposed by Darwin

A

Theory; Evolution occurs in short bursts of rapid change, followed by longer periods of stability
If evolutional change is gradual, could be predicted; fossilised remains showing these ongoing changes
Darwinists use traditional forms to support perspective of gradualism→ gradual change over an extremely long period of time
Many fossilised remains show millions of years with no noticeable evolutionary change to species
E.g. Soft bodied organisms dominates seas for hundred of millions of years and in period of million years disappeared and were replaced by organisms with shells and skeletons
Supporters of punctuated equilibrium argue if evolution occurs gradually, as proposed by Darwinists there should be greater diversity among living organisms that exist
Fossil records is incomplete so difficult to come to an agreement on evolutionary change

76
Q

Process information from secondary sources to describe and analyse the relative importance of the work of
James Watson, Francis Crick, Rosalind Franklin, Maurice Wilkins
in determining the structure of DNA and the impact of the quality of collaboration and communication on their scientific research

A

Scientists associated with double helix discovery; Rosalind given no recognition
Franklin; find out structure of DNA molecule (X-ray crystallography) → Identified A and B form
Work provided without knowledge or consent to Cambridge competitors through Wilkins; used her data to build model
Maurice Wilkins→ studied diffraction images and discovery of patterns to guess molecular DNA structure
Wilkins, Franklin diffraction images→ discovery of 3D helical nature
Crick and Watson analyse Franklin/Watson crystallography; realised DNA structure was helix
Processed infor from other, manipulated model→ Watson and Crick discovered fmoud double helix
Watson, Crick and Wilkins won Nobel Prize (1962) for work in DNA→ Franklin wasn’t honoured and appropriately acknowledged→ already dead

77
Q

Identify how the following current reproductive technologies may alter the genetic composition of a population:

  • Artificial insemination,
  • Artificial pollination,
  • Cloning
A

ARTIFICIAL INSEMINATION:
Sperm taken from chosen male; inserted into selected females
Cost effective; frozen sperm transported; not animals(reduce injury danger)
Sire several offspring with different females; conservation of species→ but reduces genetic diversity
Bred selectively; alleles increase in gene pool selected by breeder not nature
Survival and reproduction in pop→ depends on alleles that are useful to humans, not environment

ARTIFICIAL POLLINATION:
Remove stamens; dust pollen onto stigmas of another flower or same flower; E.g. Mendel pea plants
Greater degree over breeding process; ensures pure breeding→ but less genetic diversity; species vulnerable to sudden environment change

CLONING:
Reproductive cloning: Genetically identical fully developed organism using cell from other organisms
Therapeutic cloning: Cells from individual to produce cloned early embryo
Gene cloning: Producing identical copies of a gene E.g. Dolly the sheep, Bananas, twins
Characteristics can be precisely bred→ conservation; reintroduce extinct genes, endangered animals
Reduces genetic diversity; increases risk of being wiped out from sudden environmental change
Not natureal→ need to ‘switch on” genes and most experiments fail
Organisms; genetically identical→ reduced variability

78
Q

Process information from secondary sources to describe a methodology used in cloning

A

Somatic Cell Nuclear Transfer→ SCNT→ 3 animals; nucleus donor, egg donor, surrogate mother
Somatic cell nucleus transferred to egg without nucleus; reprogrammed to become zygote and grows
E.g. Dolly the sheep; cell from 6 yr old ewe udder→ injected into enucleated second sheep cell; fused with electricity→ grow and develop normally→ injected into third sheep uterus
Sheep born dentic twin to sheep donating egg

79
Q

Analyse information from secondary sources to identify examples of the use of transgenic species and use available evidence to debate the ethical issues arising from the development and use of transgenic species

A

Transgenic species: A species that has genes transferred into its genetic code from another species

E.G:
Gene from bacteria has been transferred to cotton called BT cotton→ Gene codes for bacterial protein called BT toxin that kills Helicoverpa moth→ major pest of cotton crop

Use of less insecticide on BT cotton crop→ benefits farm workers and wildlife
Increase in crop yield

FOR:

DNA and proteins of transgenic organisms unlikely to cause any problems as digested in human gut
Research suggests transgenic species don’t survive long in natural ecosystems and if genes transfer to wild species, natural selection acts against them
Transgenic techniques will reduce cost of food production, which benefits producer and consumer
Transfer of genes between species is natural phenomenon.

AGAINST:

Impossible to be certain about long-term health effects of genes in GMO foods or proteins made using genes
Genes could escape and be transferred to wild plants or animals. E.g. Possibly creating superweeds
Will allow multinational companies that develop them to become too powerful
Infringement of rights of every species to independently exists and could cause suffering in animals

80
Q

Outline the processes used to produce transgenic species and include examples of this process and reasons for its use

A

PRONUCLEAR INJECTION:
DNA can be introduced directly into an animal cell by microinjection
Multiple copies of desired transgene are injected via a glass micropipette into a recently fertilised egg cell→ then transferred to surrogate mother
Not efficient; many don’t survive

GENE GUN:
Foreign DNA into living tissues literally shoots directly into organism using “gene gun”
Microscopic particles of gold or tungsten are coated in DNA and propelled by burst of helium into skin and organs of animals and tissues of intact plants
Some of cells express introduced DNA as if it were their own.

TRANSFER USING BACTERIA TO CARRY DNA OF GENE:
E.g. Insulin
Messenger RNA coding for insulin is extracted from human pancreas cells that make insulin
DNA copies of messenger RNA made and sticky ends are made by adding extra G nucleotides to gene ends
Plasmids are small loops of DNA found in bacteria→ cut open using restriction enzymes and sticky ends made by adding C nucleotides at ends of cut plasmid
Insulin gene and plasmid are mixed. Link by complementary base pairing (G-C) between sticky ends
Plasmid with human insulin gene inserted is called recombinant plasmid
Suitable host cell is chosen to receive gene. E.g. E. Coli bacteria
Recombinant plasmid are mixed with host cells.Host cells absorb them
Genetically modified E. Coli are cultured in fermenter
E. coli bacteria start to make human insulin which is extracted, purified and used by diabetics.

81
Q

Discuss the potential impact of the use of reproductive technologies on the genetic diversity of species using a named plant and animal example that has been genetically altered

A

CLONING
Decreased genetic diversity;only one parent; identical to one parent
Desired characteristics evident;individual control and reproduced in short time
All identical→ less likely to survive sudden environmental change, foreign pathogens
May be used in conservation with endangered or extinct animals

82
Q

Discuss the difficulties of defining the terms “health” and “disease”

A

Health; “State of physical, social and mental wellbeing, not just absence of disease” WHO
Hard to achieve all 3 areas at once, different opinions about what is healthy
Disease: “Condition adversely affecting normal functioning of any part of living thing”
Broad, conditions not normally classed as disease (pregnancy, broken arm)

83
Q

Use available evidence to analyse the links between gene expression and maintenance and repair of body systems

A

Gene expression; Cell ‘switched on’ and DNA code converted to polypeptides controlling structure and function of cell
Expressed as should be→ cell functions normally, tissues maintained and repaired
Polypeptides make up proteins; some responsible for mitosis and cell cycle, or replace damaged cells
Effective gene expression needed for ongoing maintenance/repair of tissue→ if altered mutations and cells can’t function
Tumour suppressor gene→ limits cell division, encourages cell death of too many cells
BRCA1 gene→ Codes for proteins repairing PTEN gene,
PTEN gene→ limits cell divisions, encourages cell death; regulates cell cycle, prevents excessive cell production

84
Q

Outline how the function of genes, mitosis, cell differentiation and specialisation assist in the maintenance of health

A

Cell differentiation→ Cells mature, take on different structural features to suit for specific function
Cell specialisation→ Specific genes ‘switched on’ → perform particular body function
Enable cells to work together to carry out coordinated complex functions to maintain, repair tissue
Genes control production of polypeptides (make up cell proteins)→ responsible for cell growth, repair
Gene malfunction→ cells can’t function, onset of disease
Mitosis; cell division→ growth, repair damaged tissues, worn out cells→ can cause mutations; uncontrolled production of cells, prevents cell death
Mutations→ tumour suppression genes halt protein production
Disruption of cell cycle; uncontrolled cell replication→ cells don’t differentiate; forms tumours

85
Q

OVER 3000 YEARS AGO THE CHINESE AND HEBREWS WERE ADVOCATING CLEANLINESS IN FOOD, WATER AND PERSONAL HYGIENE

A

People of Mesopotamia
Drains→ carry away waste
Aware of insects carrying disease, isolated the sick

Egyptians
Performed surgery and had over 700 drugs
Specific rules for cleanliness→ house cleaning, frequent bathing, pure water

Chinese
Personal hygiene and classified diseases
Immunity→ if exposed to tissue of person with smallpox→ gain protection against more infection

Hebrews
Personal hygiene by washing and keeping clean
Water supplies kept clean, free of wastes, dead animals and people
Isolated sick and burnt used bandages

86
Q

Distinguish between infectious and noninfectious diseases

A

Infectious; disease caused by organism, infectious agent→ Ebola, Zika
Non-infectious; not caused by pathogen, can’t be passed on→ Down Syndrome, lung cancer

87
Q

Identify data sources, plan and choose equipment or resources to perform a first hand investigation to identify microbes in food or in water

A

Petri dish; innoculate bacterial colonies (e-coli) wipe in cultivated agar plate, attach antibiotic; seal
Heat, observe next day how much bacteria grown→ antibiotic with least bacteria (effective)

88
Q

Explain why cleanliness in food, water and personal hygiene assist in control of diseases

A

Hygiene→ decreases spread and growth of pathogens→ controls spread of disease

HYGIENE:
Personal hygiene
Keep body and openings clean→ reduce risk of pathogens entering
Wash hands with soap before eating, after using toilet→ prevents spread of disease
Body, hair, teeth regularly cleaned→ prevent buildup of bacteria
Cough or sneeze into handkerchief→ prevents airborne droplets spreading

Community hygiene
Sewerage, garbage disposal→ reduces increase of pathogens
Sterilisation, disinfection of equipment in hospitals, dentists→ reduces pathogen spread
City planning– reduces overcrowding→ reduce transmission of disease through pop

CLEANLINESS IN FOOD:
Pathogens transferred from person to person, or environment to person via food
Increased incidence of foodborne disease→ due to eating out on regular basis, consume takeaway
Guidelines; hair tied back, cuts covered, hands washed, utensils washed, raw veg washed, meat cooked thoroughly, food covered before stored

CLEANLINESS IN WATER:
Domestic water→ comply with strict standards and guidelines
Water contaminated with faces→ could contain unsafe pathogens
Treatment→ destroys pathogens and multiplication→ reduce transmission

89
Q

Gather, process and analyse info from secondary sources to describe ways in which drinking water can be treated and use available evidence to explain how these methods reduce the risk of infection from pathogens

A

Water treated before distribution→ ensure no health risk
Coagulation/flocculation/sedimentation/filtration→ remove matter harbouring pathogens, disinfect
Testing to ensure water meets guidelines
Water should be clear→ virtually colour free
Tested; bacteria associated with faecal contamination→ killed by chlorine
Cryptosporidium, Giardia→ may infect animals in intestines; in water through faces or animal carcass
Chlorine→ added t water to kill pathogens
Fluorine→ added to tap water to help dental health

90
Q

Identify the conditions under which an organisms is described as a pathogen

A

Any organism or infectious agent living in another organism and causing disease
Microorganisms can become one if not in normal location (E.g. good bacteria on skin if enters blood)
Methods of infectious transmission
Person to person; (blood→ HIV, Ebola)
Environment to person (common cold)
Vector; person to person via organism (Zika)

91
Q

DURING THE SECOND HALF OF THE NINETEENTH CENTURY, THE WORK OF PASTEUR AND KOCH AND OTHER SCIENTISTS STIMULATED THE SEARCH FOR MICROBES AS CAUSES OF DISEASE

A

19th century→ revolution in microbiology (pasteur and Koch) → previously spontaneous generation
P disproved theory; germ theory→ all germs cause disease, microorganisms come from pre- existing
Koch→ all disease caused by specific microorganism

92
Q

Describe the contribution of Pasteur and Koch to our understanding of infectious diseases

A

PASTEUR:
Discovered microorganisms were cause of beer, wine spoilage→ Heat them to kill (pasteurisation)
Discovered rotting food due to living organism, (refuted spontaneous generation) proposed germ theory of disease
Investigated cause of anthrax and developed successful vaccine→ also vaccine or rabies, cholera in chickens

Swan necked flask experiment
Drawn out necked flask→ meat inside and air drawn in→ microorganisms from air trapped in curved neck
No growth in curved neck, but growth in flask with broken off neck
Proved organisms contaminating broth carried in air, not spontaneously generated

KOCH:
Agar plate technique for growing microorganisms→ cultured bacteria; determined each disease caused by specific microorganism
Anthrax→ bacteria inserted into healthy sheep; showed spores obtained could cause disease→ evidence for germ theory→ microorganism grown outside body caused disease
Breakthrough→ discovery of bacteria responsible for Tuberculosis, bacteria for cholera

Postulates (principles for identifying microorganisms responsible for disease)
Same microorganism present in every diseased host
Microorganism must be isolated, cultured
Sample inoculated into host→ must display symptoms as original host
Must be able to isolate microorganisms from second host and culture and identify as original species

93
Q

Perform an investigation to model Pasteur’s experiment to identify the role of microbes in decay

A

Beef stock cubes (make broth) and conical flasks with glass tubing bent into S (replace swan neck)
Filtered broth added to flask with straight piping and one with curve
Boil broth, leave in sub and check every few days→ scum, cloudiness, fungus, bubbles
Few weeks→ straight tubing should show signs of decay

94
Q
Distinguish between 
- Prions, 
- Viruses, 
- Bacteria, 
- Protozoans, 
- Fungi, 
- Macro-parasites, 
And name one example of a disease caused by each pathogen
A

Prions
Protein capable of causing disease→ no genetic material
Multiply in contact with normal prion proteins; alter structure and change them to infectious prions
Can’t be destroyed by heating or chemicals
E.g. Kuru disease→ through cannibalism

Viruses
Non cellular pathogens, contain genetic material, not composed of cells (30-300 nm)
Only can replicate in host cells→ enters and makes copies of itself
Cell becomes too full with copies; bursts→ releases them to repeat in other host cells
E.g. Influenza, herpes, glandular fever

Bacteria
Single celled organism (0.5-100um) → reproduce by binary fusion
Found everywhere (classified on basis of shape) → many beneficial
E.g. Cause food poisoning, anthrax, pneumonia

Protozoans
Single celled eukaryotic→ cell membrane, membrane-bound nucleus and organelles (1-300um)
E.g. Malaria, African sleeping sickness

Fungi
Eukaryotic organisms; have cell wall but different to plant cell wall
Microscopic to macroscopic→ no chlorophyll (can’t produce own food)
Most live on dead plant, animal material (decomposers)
E.g. Athlete’s foot (tinea) or black spot on rose plants

Macro-parasites
Visible to naked eye; larger than other pathogens; multicellular eukaryotic
Tiny louse→ long tapeworm
Some transmit disease directly or act as vectors
Live outside host body (suck blood-mosquitos) or live inside body (tapeworm)
E.g. Flea is vector for bacteria causing plaque, plant disease→ aphids transmitting Banana Bunchy Top

95
Q

Gather and process info to trace the historical development of our understanding of the cause and prevention of malaria

A

1000 CE→ Chinese recognised disease
2000 YA→ Greeks described symptoms. Built drains to take away stagnant water
1880→ Charles Laveran discovered malaria causing pathogen
1897→ Ronald Ross discovered main stages of transmission, identified mosquito as vector
1898→ Grassi and Bastianelli→ showed human malaria is transmitted in the same way as malaria in birds
1898→ Draining stagnant water, spraying oil onto water to stop breeding, wearing protective clothes
1930→ Antimalarial drug (Atabrine) used. Discontinued due to side effects
1970’s→ Use of drugs as prophylactics to try and prevent the disease, Incidence of malaria decreased
1980’s→ Incidence of malaria increases
2000- present→ Combination drug therapy that includes highly effective aertesminsimin,. Netting treated with long term insecticide and other protective measures

96
Q

Identify data sources, gather process and analyse info from secondary sources to describe one named infectious disease in terms of its:

  • Cause,
  • Transmission,
  • Host response,
  • Major symptoms,
  • Treatment,
  • Prevention,
  • Control
A

MALARIA:

Cause
Protozoa Plasmodium→ anopheles mosquito is vector

Transmission and life cycle
Female mosquito picks up gametes of parasite from blood of host→ fertilise in mosquito gut
Zygote matures, form sporozoites (move to salivary gland) injected into another host when feeding
Sporozoites move to liver cells; grow and multiply→ cells burst→ released into blood
Transferred back to feeding mosquito

Host response
In liver cells; isolated from immune system, but in RBC→ antibodies produced
Plasmodium avoids immune response as surface antigens change weekly→ not recognised by antibodies already produced by host

Major symptoms
Cells burst→ parasite, toxins, haemoglobin breakdown released into plasma
Creates shivering, high fever, headache, sweating
Anemia→ from breakdown of RBC, haemoglobin

Treatment
Antimalarial drugs→ reduce temp or cure infection
Some resistant strains→ oral quinine instead

Prevention
Travellers to malaria areas→ drugs prior to departure and after come back→ kill any from liver
Protective clothing, insect repellent, mosquito nets
No donating blood if been recently to infected area
Ships, planes, vehicles from areas→ sprayed with insecticides

Control
Insecticides→ DDT spraying, or draining standing water
Vaccine being trialled

97
Q

Identify the role of antibiotics in the management of infectious disease

A

Antibiotics; chemicals destroy or stop growth of bacteria causing disease (without destroying host)
Destroy cell membrane, or accumulate in bacteria cells preventing them forming new cell wall
Natural selection→ evolve resistant strains. Some bacteria possess natural resistance, survive and reproduce to build up population resistant to antibiotic

98
Q

Process info from secondary sources to discuss problems relating to antibiotic resistance

A

Many no longer effective→ resistant strains forming→ dont respond to first line antibiotics; need 2nd or 3rd line (take longer to cure, more severe)
Superbugs→ virtually resistant to all; only treated with experimental drugs
Current trends→ in future, some diseases have no treatment due to resistant strains
Strategies:
Prescription only for bacterial infections, and taken for entire course (not just until symptoms stop)
Cleaning products with antimicrobial ingredients should not be used

99
Q

Identify defence barriers to prevent entry of pathogens in humans:

  • Skin,
  • Mucous membranes,
  • Cilia,
  • Chemical barriers,
  • Other body secretions
A

Skin
Prevents entry of pathogens→ microorganisms rarely penetrate intact skin
Certain bacteria on skin→ destroys incoming pathogens
Lack of moisture→ limits growth of microorganisms

Mucous membrane
Membranes line nasal passages, digestive, respiratory tracts→ secrete mucus
Mucus traps pathogens until body disposes
Prevents membranes drying → provides moist surface for normal microflora

Cilia
Fine,mucus coated, hair like→ line nasal, respiratory surfaces
Beating of cilia→ moves mucus layer along (with any trapped microorganisms) in throat

Chemical barriers
Lysozyme (enzyme) → in tears, saliva, mucus, sweat, tissue fluid→ breaks up bacteria membrane
Stomach acid (pH 1-2) → lethal environment for pathogens
Bile and acidic urine fluid→ inhibits pathogen growth

Other body secretions
Tears→ wash surface of eye when blink→ prevents settling of microorganisms
Perspiration→ any microorganism in sweat glands washed away
Saliva→ washes microorganisms from teeth, into stomach lining (digestive enzymes, stomach acid destroy)
Urine→ cleans urinary tract,flushes out microorganisms

100
Q

Gather, process and present info from secondary sources to show how a named disease results from an imbalance of microflora in humans

A

Disease/pathogen
Fungus Candida albicans → causes Thrush

Symptoms
Itching/burning sensation when urinating or sexual intercourse
Thick, white discharge
Splits in skin, redness or swelling

Treatment/ Prevention
Treatment→ vaginal creams, tablets
Prevention→ women wipe from front to back when using toilet (avoids spreading yeast from anus)

Conditions under which disease develops
Usually kept low by competition from other micro-organisms→ but if balance upset, increase in C Albicans→ disease develops
Balance upset by suppression of immune system, drug use, diabetes antibiotics treating bacterial infection→ reduced number of bacteria

101
Q

Identify antigens as molecules that trigger the immune response

A

Antigen→ molecule recognised as foreign→ triggers immune response
Surface of cells in body→ markers identify as belonging to self→ protects from immune system attack
Pathogens have chemical markers (antigens) immune recognises as not belonging to body→ activate immune response (foreign particles)

102
Q

Explain why organ transplants should trigger an immune response

A

New organ→ marker molecules on cell surface→ antigens different to markers on own cells
Organ identified as foreign→ immune response activated to attack and defend body
More number of matching macromolecules→ less foreign antigen molecules to fight

103
Q

Identify defence adaptations, including

  • Inflammatory response,
  • Phagocytosis,
  • Lymph system,
  • Cell death to seal off pathogen
A

Inflammation response
Response brought by infected/injured cells→ release chemicals for fluid containing phagocytes to enter tissues→ destroy invaders
Damage to tissue triggers response (characterised by pain, heat, swelling→ increased temp inhibits pathogen activity)
Destroys cause of infection or confines infection to small area. Replaces/ repairs damaged tissue

Phagocytosis
Phagocytes (macrophages, neutrophils) surround, enclose foreign particle→ then bathed in enzyme lysozyme (destroys it)
Neutrophils→ Immune cell, ingest bacteria in bloodstream, (then digested by lysozyme)
Macrophage→ Larger cell, wander around tissue collecting microorganisms or other foreign bodies

Lymph system
Responsible for filtering lymph fluid, (remove pathogens, dead cells, debris)
Lymph nodes contain phagocytes (destroy foreign material brought by tissue fluid)
Lymphoid organs defend body→ fight infections in spleen, bone marrow, thymus gland

Cell death to seal off pathogens
If area badly infected, other responses can’t control pathogens→ layer of dead cells forms around infection site→ followed by layer of macrophages
Seals off and contains pathogens→ eventually die and consumed by macrophages

104
Q

Identify the components of the immune response:

  • Antibodies,
  • T cells,
  • B cells
A

Antibodies
Proteins (produced by plasma cells) → in response to antigen
Antibodies seek out antigen and bind to part of it (forms antigen-antibody complex) → deactivates antigen
Antigens destroyed by antigen-antibody complex clumping together (easier to eliminate by phagocytosis)

T cells
Lymphocytes produced in bone marrow and mature in thymus gland→ released into blood, lymph nodes
Each T cell→ particular surface receptor protein (recognises specific antigen)
Encounter antigen, matching receptor→ activate and clone killer T cells→ move to infection; destroy infected cell

B cells
Lymphocytes produced and mature in bone marrow→ released into blood, lymph nodes, tonsils
On surface→ different antibody specific to antigen→ encounter antigen (makes copies of itself) → forms plasma cells that produce specific antibodies
Antibodies move to infection site→ form antigen-antibody complex (deactivate antigen

105
Q

Process, analyse and present info from secondary sources to evaluate the effectiveness of vaccination programs in preventing the spread and occurrence of once common diseases, including smallpox, diphtheria and polio

A

Smallpox
Before vaccine→ millions of deaths worldwide
1967 WHO introduces mass immunisation and follow up vaccines
Highly successful→ eradicated disease worldwide

Diphtheria
Before vaccine→ Thousands worldwide (1900’s)
1974 WHO expanded immunisation program→ aim to decrease children with disease
Still present today, but effective→ drop in global incidence to 8000 cases in 2005

Polio
Before vaccine→ Thousands of cases worldwide
1955→ Spain developed safe vaccine → 1997 Global Polio Eradication Initiative
70% reduction of cases→ rare in developed countries; but still concern in developing nations

106
Q

Describe and explain the immune response in the human body in terms of:
Interaction between B and T lymphocytes

A

Macrophage engulfs foreign particle with antigen→ antigen on surface moved to macrophage surface
Macrophage presents antigen to helper T cell in lymph nodes (has receptor corresponding to antigen)
Helper T activates→ release cytokine chemicals activating production of B clones (antigen specific)
Also activate production of cytotoxic T clones (have particular antigen receptor on surface)
Immune response defeated→ suppressor T stops B and cytotoxic cell activity

107
Q

The mechanisms that allow interaction between B and T lymphocytes

A

System in place to identify cells both belonging to body→ stop them attacking each other
Surface of cells; MHC molecules allow recognition in body and identify foreign cells
MHCI molecules present on all nucleus cells→ recognise antigens for T cell to destroy
MHCII molecules on B cells→ recognition of antigens by B cells, helps helper T cell activate B and T

Antibody- mediated immunity
B cells presenting antigens→ move to lymph nodes, inspected by helper T cells
T cells stimulate cloning of millions of B cells (specific to antigen presented)
B cells produce plasma cells→ secrete specific antibodies→ move via blood, lymph to infected area
Antigens, antibodies combine→ antigen-antibody complex (inactivates pathogens)

Cell mediated immunity
Foreign material engulfed by macrophages→ then moves to lymph nodes; inspected by helper T
Helper T activate cloning of cytotoxic and memory T cells (specific to antigen)
Cytotoxic cells go to infection→ antigen, antibody bind → release chemicals to destroy cells
Chemicals increase inflammation, attract macrophages for phagocytosis
Infection defeated→ suppressor T cells release chemicals; stop production and action of cytotoxic

108
Q

The range of T lymphocyte types and the differences in their roles

A

Memory T cell
Remain in body→ respond quickly to future invasions

Suppressor T cell
Stop immune response when infection defeated

Cytotoxic T cell
Activate and move to site of infection→ chemicals destroy infected cells

Helper T cell
Recognise antigen→ release chemicals activating cloning of T and B cells

109
Q

Outline the way in which vaccinations prevent infection

A

Vaccine→ preparation containing dead or weakened pathogens→ can’t cause disease but act as antigens; stimulate immune response and memory
Causes multiplication of B and T cells without symptoms → vaccinated person gains immunity; when actual disease occurs→ body undergoes immune response and produces antibodies for that antigen

110
Q

Outline the reasons for the suppression of the immune response in organ transplant patients

A

Donor organ→ marker molecules act as antigens (seen as foreign material) immune response initiated
Cytotoxic T cells activated→ attack cells, reject organ
To reduce severity of immune response; tissue of donor and recipient need to be closely matched
Drugs to suppress immune system to lower risk of rejection
Drugs reduce activity of T cells (main cells attacking) → advantage; whole immune system not suppressed, can still defend the body→ but not as effective
Much greater risk of infection (anti-rejection drugs needed for rest of life)

111
Q

Identify and describe the main features of epidemiology using lung cancer as an example

A

Study of patterns of disease occurrence in human pop, hypothesises cause and strategies to control
Results used by public health authorities to develop strategies and evaluate ones already in place
Descriptive studies→ frequency of disease, geographical location, time period, who is affected
Analytical studies→ Statistics on mortality rates, incidence and prevalence
Intervention studies→ Test effectiveness of treatment or campaign
LUNG CANCER
Data collected on age, sex, smoking habits of smokers and nonsmokers
Case studies compare lung cancer and those without→ look for exposure (suggests smoking)
Cohort studies compare non smokers and smokers to establish link between lung cancer→ greater number of cigarettes smoked daily; greater chance of dying from lung cancer
Look at effectiveness of Quit campaign

112
Q

Gather, process and analyse info to identify the cause and effect relationship of smoking and lung cancer

A

Cancer council→ 1 in 8 cancers from smoking (most common; lung cancer)
1 in 5 cancer deaths from smoking→ smoking biggest factor for preventable cancer
High risk of developing when exposed to cigarettes→ more smoked; greater risk of cancer

113
Q

Identify causes of noninfectious disease using an example from each of the following categories:

  • Inherited diseases,
  • Nutritional deficiencies,
  • Environmental diseases
A

Inherited diseases
Genetically transmitted; errors in genetic infi
May be change in chromosome number or defect in single gene (mutation)
E.g. Cystic fibrosis→ mutation to gene causes secretion of thick mucus
Blocks passageways into lungs, digestive tract→ problems with breathing, nutrient absorption. No cure.

Nutritional deficiencies
Unbalanced diet,or physiological conditions leading to poor diets (anorexia)
E.g. Scurvy from not getting enough vit C, or anemia from missing iron

Environmental diseases
Lifestyle related (substance abuse) or physical environment (exposure to uv rays)
Disease caused from exposure to chemicals→ e.g. asbestos causes mesothelioma
Cancer of cells in membrane on outside of lung→ asbestos fibres penetrate lining and damage cells causing mutations→ mesothelioma

114
Q

Identify data sources, plan and perform a first hand investigation or gather info from secondary sources to analyse and present info about the occurrence, symptoms, cause, treatment/management of a named non-infectious disease

A

SCURVY
Occurrence
Rare in developed countries→ mainly in developing where fresh fruit and veg not freely available
Occur in all age groups→ alcoholics, elderly, infants

Symptoms
Swollen, purplish gums
Swelling, painful legs
If left untreated; gangrene, haemorrhaging, death

Cause
Nutritional deficiency→ lack of vit C (needed for connective tissues, bones)
Deficiency weakens blood capillary wall→ bleeding, bruising

Treatment/management
Increase amount of vit C in diet→ or vit C supplements
Prevention→ eat foods rich in vit C (orange, lemons,cranberries)

115
Q

Discuss the role of quarantine in preventing the spread of disease and plants and animals into Australia or across regions of Australia

A

Quarantine→ isolation of organisms to control spread of disease
All animals coming into Australia→ required to spend time in quarantine (ensure free of disease)
Policy protects health of humans, environment→ reduced rate of disease incursion
Prevents introduction, establishment or spread of disease/pests
E.g. Rhinos from Africa commonly have internal parasites; could transfer to native fauna

116
Q

Perform an investigation to examine plant shoots and leaves and gather first hand evidence of pathogens and insect pests

A

Lemon plant
Pest→ insect leaf miner
Insect eats leaf layers→ causes leaf to curl

Rose bush
Fungal pathogen→ Black spot
Yellowing of leaves, black powdery mildew

Rose bush
Pest→ Aphid
Small leaves, stunted leaf growth

117
Q

Explain how one of the following strategies has controlled and/ or prevented disease:
Public health programs,

A

Govt regulations; Sterilising hospital equipment, cook/prepare food→ prevents spread of pathogens
Govt regulations; garbage disposal, drinking water and sewerage treatment→ prevents occurrence
Laws for reporting diseases→ allows early detection and strategies to be implemented
Encourage regular screening for disease; e,g women check breast for lumps (cancer detection)
Childhood immunisation program→ prevent disease incidence (e.g. whooping cough)
Education programs; awareness of lifestyle risk factors (E.g. Quit campaign→ images of side effects)

118
Q

Process and analyse information from secondary sources to evaluate the effectiveness of quarantine in preventing the spread of plant and animal disease into Australia or across regions of Australia

A

Many plant species refused entry; or allowed in if treated to ensure no pests
Some restrictions on movement of fruit → prevents spread to disease free areas
Quarantine checkpoints on main roads leading to each state (prevents risk material entering)
If disease enters Aus; quarantine, control of movements, slaughter or vaccination of infected animals
Observation and decontamination→ establish disease free areas
E.g. Swine Flu 2007→ horse movement banned, equipment decontaminated, → no swine Flu 2008

119
Q

Gather and process information and use available evidence to discuss the changing methods of dealing with plant and animal diseases, including the shift in emphasis from treatment and control to management or prevention of the disease.

A

TREATMENT AND CONTROL OF DISEASE:

Use of pesticides
Controls spread of vectors and pests; E.g DDT to kill mosquito (malaria)
Harms environment and can lead to resistant strains developing

Use of antibiotics
Treats many type of bacterial infections; destroys bacteria and cures person
Overuse and wrongful use → development of resistant strains

Medical intervention procedures
E.g. Cancer treatment by surgery radiation, chemotherapy→ if successful cures patient
Side effects of treatment severe and increase suffering of patient

PREVENTION AND MANAGEMENT OF DISEASE:

Vaccination
Prevents person from contracting the disease; decreases prevalence of disease
E.g. vaccination against diphtheria; only small percentage suffer side effects

Quarantine
Prevents entry into Aus; management procedures prevent spread around country
Decreases incidence of disease and protects agricultural industry allowing them entry to export markets with disease free products

Genetic engineering
Plants and animals produced that are resistant to pathogens/pests→ prevents occurrence of disease
Reduces need for spraying crops with pesticides
Effects on biodiversity unknown; could be harmful
E.g. Bt cotton that produces natural insecticide to kill caterpillar larvae

Public health programs
Reduces incidence of disease in population
E.g. public education programs; no smoking to prevent lung cancer

120
Q

Identify the role of receptors in detecting stimuli

A

Stimulus→ Change in internal or external environment; detected by receptors, response triggered
Receptors: single cells or concentrated in areas to form sense organs (eye, ear)
E.g. Touch hot plate, receptors in skin detect heat and pain cause to withdraw fingers→ coordination needs link between receptors and effectors (muscles or glands that respond)
Link carried out by nervous system
Photoreceptor→ sensitive to light energy (visible light, UV light)
Mechanoreceptors→ Mechanical energy (touch, pressure, Gravity)
Thermoreceptors→ respond to heat and cold (changes in temp)

121
Q

Identify data sources, gather and process information from secondary sources to identify the range of senses involved in communication

A

Communication: sending and receiving meaningful info
Communicator (sending info) needs to have signalling device. E.g. Human voice box for sound production
Recipient (receiving signal) needs sensory structure to detect signal. E.g. Human ears to detect sound
Animals use all 5 senses→ communication relies on corresponding visual, acoustic, tactile (touch) and chemical (smell and taste) receptors.

Sight→ Lion; Hairs on mane stand up (larger); when another male in environment
Sound→ Lion; Roar to intimidate other male lion; aggressive warning
Taste→ Ants follow pheromone markers left by others to indicate location of food
Smell→ Fish emit odours to establish rank in a social group
Touch→ Humans hug and shake hands to greet

122
Q

Explain that the response to stimuli involves:

Stimulus, Receptor, Messenger, Effector, Response

A

CNS triggers response to stimulus; receptors connected by nerves
Receptors change stimuli into electrochemical signals (nerve impulses)
Travel along nerves, transmit messages to CNS from receptors→ processed and interpreted as info and response initiated
CNS sends impulses along nerves to effector organs (carry out response)
Brain and spinal cord interpret and make sense of messages (take into account past experiences or as inherited reflex)

123
Q

Describe the anatomy and function of the human eye, including the:

  • Conjunctiva,
  • Cornea,
  • Sclera,
  • Choroid,
  • Retina,
  • Iris,
  • Lens,
  • Aqueous and vitreous humour,
  • Ciliary body,
  • Optic nerve
A

Conjunctiva
Thin transparent membrane (continuous with inner layer of eyelid)→ protects front of eye

Cornea
Transparent; allows light to enter eye. No blood vessels
Curvature→ helps bend/refract incoming light rays to converge and land at back of eyeball
Nerve fibres sensitive to touch and pain

Sclera
Outermost layer of eye→ protects inner layers (non-elastic, tough tissue)
Site of attachment for external muscles; enables eye movement in socket
Maintains shape of eye; transparent at front, opaque at back forming white part of eye

Choroid
Middle coat of eyeball→ most of blood vessels in layer
Back layer is black; reduce scattering/reflection of light within eye
Front layer continues to form ciliary body and lens

Retina
Thin, delicate, contains several layers of nerve cells
Contains photoreceptors  (rods, cones) → respond to light, convert light energy into nerve impulses

Iris
Coloured part of eye; made of connective tissue, smooth muscle→ muscles control size of pupil

Lens
Transparent, biconvex in shape (bulges outwards) → refracts light rays. Directs them onto retina (where focused image will be formed)
Elasticity; vary from round to flatter surface; eye accommodates near and far vision

Aqueous and vitreous humour
Transparent watery liquid (like blood plasma)
Contains dissolved nutrients
Aqueous; Provides nutrients for cornea and lens that don’t have own supply
Vitreous; Material fills remainder of eyeball; helps maintain shape and provide nutrients

Ciliary body
Muscles and ligaments; help adjust curvature of lens
Ciliary muscles relax; lens flat (distant vision)
Muscles contract: lens rounder (near vision)

Optic nerve
Nerves pass through skull via opening in eye socket→ carry electrochemical signals from retina to brain

124
Q

Use available evidence to suggest reasons for the differences in range of electromagnetic radiation detected by humans and other animals

A

HUMANS
380-760nm→ all colours in visible light (no uv range)
Active during day→ need to distinguish colours in environment

INSECTS
Bee→ 300-650nm→ uv range, blue, green (not red)
UV patterns on flowers to attract bees to pollen and nectar

Ant→ 320-630nm→ uv, blue, green. yellow range
Maximum sensitivity; discriminate between colours in environment, when gathering food

VERTEBRATE
Snake→ 400-850 nm→ blue, green, red, uv→ relies on infrared to locate prey in dark burrows

Pigeon→ 460-700 nm→ blue, green, red→ distinguish environment when flying

125
Q

Identify the limited range of wavelengths and the electromagnetic spectrum detected by humans and compare this range with those of other vertebrates and invertebrates

A

Visible light; small part of electromagnetic spectrum seen by humans; (rods, cones)
Humans visible wavelength: 380-760nm
Objects absorb some wavelengths, reflect other→ objects look coloured because of light they reflect
E.g. Green lead; green light reflected. Other colours (red, blue etc) absorbed; can’t be detected by eye
Humans can’t detect ultraviolet part of spectrum; sensitive to light in red, green, blue. (see all colours)
Insects see ultraviolet range; sensitive cells for shorter wavelengths
Birds see ultraviolet range, detect most efficiently in red and green ranges
Some flying animals detect polarised light; used for navigation in flight

126
Q

Identify the conditions under which the refraction of light occurs

A

Refraction: bending of light as it travels from one medium to another (air to water)
Light rays move through more dense medium, it slows down and bends towards normal
Light passes through biconvex (eye lens) → light rays refracted to focal point (focus point)
Refracted light through biconvex lens converge at focus point

Convex lens→ converge. Concave lenses→ diverge
Lens in eye changes shape→ allows eye to form focused image of near and far objects
Light refracted at cornea, aqueous humour, lens and vitreous humour→ curvature of cornea and lens refracts the light rays to converge and form an image on the retina at the back of the eye

127
Q

Plan, choose equipment or resources and perform a first hand investigation to model the process of accommodation by passing rays of light through convex lenses of different focal lengths

A

Light box and different focal lengths from concave and convex
Convex lens → light rays from box converges
Concave lens→ light rays from box diverges

128
Q

Identify the cornea, aqueous humor, lens and vitreous humor as refractive media

A

Density of cornea, aqueous humour, lens and vitreous humour→ all similar and close to water
All structures refract light passing through eye to a greater or lesser degree
Refractive power of air: much lower than refractive power of eye parts
Light oases from air into refractive surfaces
Therefore→ Greatest degree of refraction in human eye occurs at boundary of air and cornea→ difference between air and cornea is greater than the difference between internal eye structures

129
Q

Plan, choose equipment or resources and perform a first hand investigation of a mammalian eye to gather first hand data to relate structures to functions

ADDDDDD INNNNNNNNN

A

ADD IN

130
Q

Analyse information from secondary sources to describe changes in the shape of the eye’s lens when focusing on near and far objects

A

Distant vision→ flat curvature (ciliary muscles relax and pull suspensory ligaments taut)
Near vision → increased curvature (ciliary muscles contract and suspensory ligaments slacken) Lens becomes rounded; greater refraction

131
Q

Process and analyse information from secondary sources to describe cataracts and the technology that can be used to prevent blindness from cataracts and discuss the implications of this technology for society

A

CATARACTS
Clouding of lens; obstructs path of light into eye and transmission of clear signals
Part of lens becomes opaque; blurred vision, looking through ‘veil’
Form when proteins build up in lens; new cells form outside lens and old cells compacted into centre
Due to ageing or injury to eye

TECHNOLOGY
Phacoemulsification: Probe inserted via tiny incision; breaks cataract into pieces; then suctioned out
Lens implant permanently inserted into eye; focuses light rays on surface of retina. No stitches needed
Extracapsular extraction: Large incision; removes centre of cataract in one piece; stitches needed

IMPLICATIONS FOR SOCIETY
Millions have vision restored; ends avoidable blindness
Rarely have any complications; majority return to daily activities
Increases life expectancy, gives more independence, patients see shaper focus, brighter colours
Patients can return to work, regain self sufficiency→ allows caretakers (family) to return to work
Previously→ removal of entire lens, have to wear thick glasses

132
Q

Compare the change in refractive power of the lens from rest to maximum accommodation AND Identify accomodation as the focusing on objects at different distances, describe its achievement through the change in curvature of the lens and explain its importance

A

Change in curvature of lens→ accommodation (used at looking at near or far objects)
Degree of lens curvature determines degree of light refraction
Increased lens curvature (thicker lens) decreases focal length (increased refraction)
Decreased curvature (thinner lens) increases focal length (decreased refraction)
Refractive power changes from low (thin, flat lens) when at rest (distant objects) to high (round lens) at maximum accommodation (near objects)
Distant vision→ flat curvature (ciliary muscles relax and pull suspensory ligaments taut)
Near vision → increased curvature (ciliary muscles contract and suspensory ligaments slacken) Lens becomes rounded; greater refraction

133
Q

Distinguish between myopia and hyperopia and outline how technologies can be used to correct these conditions

A

MYOPIA
Short sightedness→ distant objects unclear, but close up objects seen well
Distant objects image fall in front of retina; not on it→ light rays bent incorrectly; blurred vision
Occurs when eyeball too long for lens, or cornea/lens too curved for eyeball length

HYPEROPIA
Long sightedness→ close up objects unclear but distant objects seen well
Image falls behind retina; not on it→ light rays not bent to single focus→ blurred vision
Occurs when eye too rounded, or lens too flat to alter light adequately

CORRECTIVE TECHNOLOGIES
Corrective lenses from glasses or contact lenses→ shaped to fit eyeball curvature
Myopia: Concave lens (thicker outwards, thinner inwards) bends light outwards→ light diverges before meets eye; extends focal length
Hyperopia: Convex lens (thinner outwards, thicker inwards) bends light inwards → light converges before meets eye; shortens focal length
Laser eye surgery to change cornea curvature and alter refraction
PRK→ Outer cornea surface removed; laser shapes uppermost surface of cornea
LASIK→ Cut and lift flap of cornea and reshape using lasers before replacing flap
Orthokeratology→ Wear special lens at night to reshape cornea while asleep; in morning temporarily retains new shape; people can seen without glasses

134
Q

Explain how the production of two different images of a view can result in depth perception

A

Depth perception; Ability to determine distances between objects and see the world in 3D
Judge distances→ from 2 forward facing eyes; brain interprets the 2 images from each eye (by superimposing them to produce a single binocular vision)
Brain uses slight differences in the 2 images to create 3D vision allowing calculate depth
DP also occurs from previous experience of the size of an object
When moving the head; the distant objects move less compared to close objects