Biologics Flashcards
What are the 4 broad categories of biologics for Psoriasis?
TNF
17
23
12/23
List 5 TNF-alpha inhibitors:
Adalimumab/humira
Infliximab/remicade
Etanercept/Enbrel
Certolizumab/cimzia
3) Given an example of an IL-12/23 inhibitor:
stelara/ustekinumab
4) List 4 IL-17 inhibitors:
Ixekizumab-talz
Brodalumab- siliq
secukinumab- cosentyx
Bimekizumab (Bimzelx)
5) List 3 IL-23 inhibitors:
Guselkumab (Tremfya)
Rizankizumab (Skyrizi)
Tildrakizumab (Ilumya)
Humira - 1) What is the source and structure?
Fully human monoclonal IgG1 ab
Humira- What is MOA?
a. Adalimumab binds specifically to TNF-alpha and blocks its interaction with the p55 and p75 cell surface TNF receptors. Adalimumab also lyses surface TNF-expressing cells in vitro in thepresence of complement.
b. *unclear on membrane bound tnf alpha
What is pregnancy category for humira, infliximab and etanercept
Class B
7) List 7 FDA approved indications for humira
Psoriasis
HS
Chrons-adult and paediatric
UC
PsA
Ankylosing spondylitis
RA
polyarticular JIA
Uveitis
List 5 off-label uses humira
PG
Behcets
Sarcoidosis
Vasculitis
PRP
How is it supplied?
a. Single use pens or single use prefilled syringes, given SC
10) What is the starting dosing? For Psoriasis vs. HS? What is the maintenance dose? for Humira
Psoriasis:
80 mg day 0, 40 at week 1, then 40 mg q2 weekly
HS: 160 day 0, then 80 week at 2 weeks, then 40 mg at 4 weeks, then 40 mg weekly
What baseline blood work would you order before starting TNFs
CBC diff, Liver enzymes, Cr/urea
Hep B serology
Hep C serology
TBST + CXR or IGRA + CXR
Strongy serology
12) What is the PASI-75 of Humira at 16 weeks?
Infliximab at 10 weeks? Etanercept at 10 weeks
Humira- 70%
Infliximab-80%
Etanercept- 50%
(12 weeks 50%, 24 wks60%)
13) How quickly does humira work? Onset of response?
Infliximab?
Etanercept?
1-2 weeks Humira
2 weeks infliximab
4 weeks etanercept
14) What is the bioavailability of humira? Inflximab? Etanercept?
64%- humira
100% - infliximab
58% etanercept
15) After how long are peak levels achieved for humira? Infliximab? Etanercept
5.5 days- humira
infliximab-immediate post infusion
Etanercept 2 days
Half life of humira? infliximab? Etanercept?
14 days- humira
7-9 days infliximab
5 days etanercept
17) How is infliximab, humira and etanercept metabolized?
proteolysis
18) How are humira, infliximab and etanercept excreted?
Fragments into bile and urine
Infliximab - What is the source and structure?
a. 25% mouse, 75% human chimeric IgG1 monoclonal Ab
What is the mechanism of action of infliximab
Binds to soluble and membrane bound TNF alpha
The antibodies also activate complement mediated cytotoxicity and induce cellular apoptosis.
List 10 adverse effects of infliximab, humira and etanercept
Infusion reaction
Allergic reaction including anaphylaxis
Increased risk reactivation TB, HepB
Increased risk serious and OIs
Increased risk malignancy-NMSC and lymphoma
Increased risk of new onset or worsening demyelinating disease
Increased risk new onset or worsening CHF
Drug induced ANA+ and lupus
Drug induced vaculitis
Drug induced lupus
paradoxical pso
Cytopenias
Differences:
-increased risk AI hepatitis and hepatoxicity infliximab
-Etanercept high risk injection site reactions, increased risk demyelinating d/o
What are absolute contraindications? List 3 for infliximab, humira and etanercept
Known hypersensitivity to infliximab or murine proteins
Concurrent administration with anakinra (IL-1 receptor antagonist)
Active infections/Chronic or localized infections—including TB
5) What are relative contraindication? List 2 for infliximab, humira and etanercept
Moderate to severe (New York Classification III or IV) congestive heart failure (OK with doses ≤5 mg/kg)
Family history of demyelinating disease (including multiple sclerosis)
7) List 5 FDA approved indications for infliximab
Pso
PsA
Chrons
Colitis
Ank Spond
RA
JIA
*Not HS, not Uveitis
8) List 10 off-label uses infliximab and etanercept
1, 2. Neutrophilic (PG, Behcets)
3, 4, 5 , 6Granulomatous (sarcoidosis, GA, granulomatous cheilitis, necrobiosis lipoidica diabetocorum),
7, 8Vasculitis ( GCA, GPA),
9,10Autoinflammatory keratinization d/o (HS, PRP),
11 AI-CTD (DM),
12-14. Others: Reactive arthritis, GVDH, multricentric reticulohistiocytosis
SJS/TEN
How is infliximab supplied
Lypophilized powder for reconstitution, single use vial, infusion IV
10) What is the starting dosing for infliximab? Maintenance?
3-5 mg/kg at 0,2,6,8 weeks then q8
20) Mean time to relapse after discontinuation infliximab
12 weeks
Source and structure etanercept
Fully human dimeric fusion protein: 2 binding domains of p75 receptor bound to Fc portion of IgG1
Produced in Chinese hamster ovaries
MOA etanercept
Binds TNF-alpha and TNF-beta, soluble and transmembrane
Name FDA indications etanercept
Pso
PsA
RA
JIA
Ank spond
*Not IBD
8) How is it supplied?
Prefilled syringes, autoinjector, lypophilized powder requiring reconstitution
Dosing for etanercepts
50 mg SC twice weekly x12 weeks then
50 mg SC weekly
19) Mean time to relapse after discontinuation? Etanercept
12 weeks
20) List 7 live vaccine:
Mumps
Measles
Rubella
Varicella
Yellow Fever
Intranasal flu
Old shingles (Zostavax)
BCG
Oral polio/typhoid
Rotavirus
What enzyme cleaves membrane bound TNF-alpha into its more potent soluble form?
TNF alpha converting enzyme
2) What are the two TNF-alpha receptors? Where are they found?
P55-most cells, epidermal cells –> tnf alpha action usually through this
P75- immune cells only
Which TNF highest risk injection site rxn
etanercept
4) Most likely to induce ANA positivity.
Inflix
5) Showed an increased incidence of serious infections when used with anakinra. *
a. Etancercept
6) Concomitant use of methotrexate can reduce its clearance by 44%.
Adalimumab
10) Mean terminal half-life is 14 day
Ada
11) Least likely to have associated tachyphylaxis.
Etanercept
12) Most likely to stimulate the development of human anti-human antibodies.
Ada
13) Least likely to cause hepatitis
Etanercept
15) Most associated with the development of histoplasmosis.
Infliximab
16) Least likely to have measurable anti-drug antibodies.
etaner
17) Least likely to reactivate tuberculosis.
Etaner
18) Mean terminal half-life is 7 days.a.
Infliximab
19) Does not fix complement binding both membrane and soluble forms of TNF
a. Certolizumab
When giving a live vaccine, should wait 42-70 days, give vaccine, wait another 10-14 days and then restart drug
a. Adalimumab
(half life 2 weeks
21) When giving a NON live vaccine, must wait 10-14 days, give vaccine, wait another 10-14 days and then restart
adalimumab
22) Binds to lymphotoxin B.
Etanercept
23) Results in a PASI75 of 80% at week 10 with over one half of patients achieving a PASI90.
infliximab
MOA CERTO, STRUCTURE AND FUNCTION
Certolizumab pegol (CZP) is a recombinant, humanized antibody fragment composed of a Fab’ antibody fragment conjugated with polyethylene glycol (PEG) that acts by neutralizing both membrane-bound and soluble TNF-α.
*DOES NOT FIX COMPLEMENT (DIFFERS FROM others)
MOA ALL 4 TNF inhibitors:
Adalimumab:
-Binds soluble TNF-alpha, “prevents interaction with p55/-75 TNF transmembrane receptor”
-fixes complement and it can lyse cells that express TNF-α on their surface
*unclear re: membrane bound tnf alpha”
Infliximab:
- Solublizes soluble TNF alpha and blocks membrane bound TNF-alpha,
-fixes complement and can lyse cells that express TNF-α on their surface
Etanercept:
-Binds soluble AND transmembrane TNF-alpha + also binds TNF-beta.
Certolizumab:
- binds both membrane-bound and soluble TNF-α.
-does NOT fix complement bc it does NOT contain Fc
most associated AI hepatitis
infliximab
25) Given subcutaneously at 80 mg followed by 40 mg one week later and then 40 mg every two weeks thereafter.
ada
x26) Contraindicated in patients with a hypersensitivity to murine proteins
inflicting
27) Full human monoclonal antibody that can fix complement and inhibits binding to transmembrane TNF-alpha
ada
28) Binds both membrane-bound and soluble forms of TNF-alpha and binds complement
influx and ADA
31) Produced in Chinese hamster ovary cells.
Etan
32) Associated with human anti-chimeric antibodies.
Inflixi