Biological Safety Cabinets

Question 1

Class 1

Answer 1

• open fronted, air brought in from room through and passed through HEPA filter
• may be vented internally or externally
• at least 1 HEPA filter
• used in high schools, and pharmacies

Question 2

Class 2

Answer 2

• partially contained cabinet with a glass sash leaving 8-10 inches open for working
• min 2 HEPA filets
• may be vented outside or back into room
• high degree of protections
• used in non- research medical labs

Question 3

Class 3

Answer 3

• completely sealed and exhausted to outside after HEPA filtration
• air from outside is filled before brought in thru HEPA
• always exhaust bac outside
• used for airborne contaminants , TB, Ebola, anthrax, research
• at least 2 HEPA filters, usually 3

Question 4

Fume Hood

Answer 4

• used for chemical storage and work to draw irritating or toxic chemicals up and away from operator
• no HEPA filters- no biological protection
• used for parasite and histology processing, reagent preparations, or is solvent is toxic or flammable

Question 5

Cleaning Safety Cabinets

Answer 5

• done using alcohol (bleach will cause corrosion)
• can use gluteraldehyde - inhalation irritant * must have cabinet running which defeats the purpose if vented back to room*
• if vented to room must use 70% isopropyl alcohol - HEPA filters checked annually
• have have UV light installed for sterilization