Biological molecules and food tests Flashcards

1
Q

Carbohydrates contain?

A

C H O

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2
Q

Proteins contain

A

C H O N

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3
Q

lipids contain ?

A

C H O

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4
Q

Maltose goes to

A

Starch

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5
Q

Amino acids goes to

A

proteins

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6
Q

glycerol and fatty acids goes to

A

lipids

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7
Q

How to make a food sample

A
  1. get a piece of food and break it up with a pestle and mortar
  2. transfer food to beaker and add distilled water
  3. stir mixture with glass rod to dissolve some food
  4. filter solution using funnel and filter paper to get rid of solid.
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8
Q

Glucose food test ?

A

Benedicts test

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9
Q

Starch food test ?

A

Iodine solution

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10
Q

Protein food test ?

A

Biuret test

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11
Q

benedicts test

A
  1. prépare food test and transfer 5cm3 to test tube.
  2. prépare water bath at 75degrees.
  3. add benedict solution (10 drops)
  4. leave test tube in water bath for 5 minuted
  5. if contains glucose - solution will change from blue to green/yellow in low conc amd brick red in high conc
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12
Q

iodine solution test

A
  1. make food sample and transfer 5cm3 to sample
  2. add few drops of iodine solution and gently shake.
  3. if sample contains starch - colour will change from browny orange to black or blue black
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13
Q

biuret test

A
  1. prépare sample and transfer 2cm3 to test tube.
  2. add 2cm3 of biuret solution and mix contents by gently shaking.
  3. if contains protein - colour change from blue to pink/purple
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14
Q

lipid food test ?

A

sudan III Test

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15
Q

sudan III test

A
  1. prépare sample but don’t need to filter. transfer 5cm3 into test tube.
  2. add 3 drops of sudan III stain solution and gently shake
  3. if contains lipids - mixture will séparate into 2 layers. top layer will be bright red.
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