biological molecules Flashcards
chemical elements in carbs
HOC
Carbon
Oxygen
Hydrogen
chemical elements in proteins
PHONCS Phosphorus Hydrogen Oxygen Nitrogen Carbon Sulphur
chemical elements in lipids
HOC
Hydrogen
Oxygen
Carbon
structure of carbs
as large molecules made up of smaller units
many glucose molecules –> starch/ glycogen
structure of proteins
as large molecules made up of smaller units
many amino acids
structure of lipids
as large molecules made up of smaller units
1 glycerol molecule
3 fatty acids
test for glucose (sugar)
1) add benedicts solution
2) heat
\+ve= orange/red -ve= blue
test for starch
add iodine
\+ve= bluey black -ve= orange brown
role of enzymes as catalysts in metabolic reactions
the substrate fits onto the active site of the enzyme (protein molecule) (like a piece of puzzle) creating an enzyme-substrate complex
the substrate then splits as products leaving the enzyme unchanged.
enzymes need to break down starch, proteins and fats because they are too big to pass through the wall of the gut and into the blood.
protein digestion needs what enzyme
protease to make amino acids
lipid digestion requires what enzyme
lipase to make fatty acids & glycerol
carbohydrate digestion requires what enzyme
it needs 2! 1) amylase to make maltose 2) maltase to make glucose
how does temperature affect the rate of enzyme- controlled reactions
C- put starch and amylase mixture in water baths of 30, 40, 60, 80*c and on ice
O- use same volume of amylase each time and keep the conc of amylase the same
R- repeat 3 times at each temp
M- time taken for the iodine not to change colour
M- test every 30 secs
S- keep the pH the same.
as the temp goes up, the time goes down, meaning it reacts faster up to 60*c because it then denatures.
temp affects on enzyme activity
the rate of reaction increases between 0-40c because the enzyme-substrate molecules have more kinetic energy and are more likely to collide.
40c is the optimum temp
(human temp 37)
above 40*c enzymes denature and substrate molecules can no longer fit into their active sites
pH effects on enzyme activity
optimal pH is 8
stomach= acidic (HCL)
small intestine= alkaline
denature, cant bind, cant catalyse.