Biofilm Flashcards

1
Q

Basic properties of biofilm

A
  1. Cooperating community
  2. Microcolonies
  3. Protective matrix
  4. Differing environments
  5. Resistant to antibiotics, antimicrobials, and host defence
  6. Bulk of biofilm made of water
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2
Q

Basic composition of plaque

A
  1. Microorganisms(mycoplasma, yeast, protozoa, viruses)
  2. Host cells (macrophages, leukocytes, epithelial cells)
  3. Organic compounds (polysaccharides, glycoproteins)
  4. Inorganic compounds (calcium, phosphorus, sodium)
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3
Q

Classification of dental plaque

A
  1. Marginal plaque
  2. Supragingival plaque
  3. Subgingival plaque
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4
Q

Supragingival plaque (aerobic)

A
  1. S. sangunis
  2. S. mutans
  3. Actinomyces viscosus
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5
Q

Subgingival plaque (anaerobic)

A
  1. Fusobacterium spp.
  2. Prevotella intermedia
  3. Anaerobic spirochetes
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6
Q

Red complex

PTT

A
  1. Porphyromonas gingivalis
  2. Treponema denticola
  3. Tannerella forsythia
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7
Q

Orange complex

A
  1. Prevotella spp.

2. Fusobacterium spp.

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8
Q

Yellow complex

A

S. mitis, S. oralis, S. sanguinis

S. gordonii, S. intermedius

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9
Q

Factors favouring plaque accumulation

A
  1. Pits and fissures
  2. Malocclusion
  3. Overhanging restorations
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10
Q

Plaque (biofilm) formation

A
  1. Association
  2. Adhesion
  3. Proliferation
  4. Micro-colonization
  5. Biofilm maturation
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11
Q

Association

A

Formation of acquired pellicle (Thin layer of salivary glycoproteins and GCF) . Hydroxyapatite crystals

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12
Q

Adhesion

A

Reversible attachment to pellicle by Van der waals and electrostatic forces. Fimbrae attaches to proline rich proteins (receptors)

Initial colonizers include Streptococcus and Actinomyces spp. Gram positive rods and cocci
Initial colonizers derive nutrition from glycoproteins and simple sugars

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13
Q

Proliferation

A

Lateral growth to cover tooth surface

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14
Q

Microcolonization

A

S. mutans produce extracellular polysaccharide like glucans and fructans which aid in adhesion of more bacteria and as an energy source for more colonies

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15
Q

Biofilm maturation

A

Secondary colonizers attach by co-aggregation

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