Biochem Basics Flashcards
Any substance that resists a change in pH when protons are produced or consumed
Buffer
Negative logarithm of hydrogen ion concentration
pH
Chemical compound that has total net charge of zero
Zwitterion
pH at which the zwitterion is the predominant form of the chemical compound
Isoelectric pH
(pK before + pK after) / 2
Bond between a water molecule and another polar compound
Hydrogen bond
Bond between glucose and galactose in lactose
Glycosidic bond
Primary bond of glucosyl residues in glycogen
Alpha (1->4) glycosidic bind
Branching bond of glucosyl residues in glycogen
Alpha (1->6) glycosidic bond
Bond between glycerol and its fatty acid
Ester bonds
Dissolved by lipase
Bond between serine and alanine in a protein
Peptide bond
- it’s a partial double bond
Bond between two cysteine residues in cystine
Disulfide bond
- also bond in immunoglobulins
Bond between ribose and adenine in adenosine monophosphate
Beta N glycosidic bond
Bond between nucleotides on a DNA strand
Phosphodiester bond
-dissolved by phosphodiesterase
Bond between C-G and A-T
A-T. 2 hydrogen bonds
C-T. 3 hydrogen bonds
Equation used to calculate the concentration of a weak acid and its conjugate base
Henderson hasselbach equation
Equation describes how reaction velocity varies with substrate concentration
Michaelis-Menten
This is a double reciprocal plot used to calculate Km and Vmax, as well as to determine the mechanism of action of enzyme inhibitors
Lineweaver Burke plot
Explains how the free energy generated by the transport electrons through the ETC is used produce ATP from ADP+Pi
Mitchell Chemiosmosmotic Hypothesis
Explains oxidative phosphorylation
Accepts H+
Base
Donates protons
Acid
pKa below 7
Acid
pKa above 7
Base
Carbohydrate, Protein, Lipid or Nucleic Acid?
Glycogen
Carbohydrate
Carbohydrate, Protein, Lipid or Nucleic Acid?
cAMP
Nucleic acid
Carbohydrate, Protein, Lipid or Nucleic Acid?
Collagen
Protein
Carbohydrate, Protein, Lipid or Nucleic Acid?
Palmitate
Lipid
Carbohydrate, Protein, Lipid or Nucleic Acid?
Inosine Monophosphate
Nucleic acid
- it’s the parent protein
Carbohydrate, Protein, Lipid or Nucleic Acid?
Sorbitol from glucose reduction
Carbohydrate
Carbohydrate, Protein, Lipid or Nucleic Acid?
Vitamin A
Lipid
Carbohydrate, Protein, Lipid or Nucleic Acid?
Insulin
Protein
Substrate concentration at which the reaction velocity is equal to 1/2 Vmax
Michaelis constant
Maximal velocity of an enzyme catalyzed reaction
Vmax
The structure of the inhibitor resemblance the substrate
Competitive inhibition
Vmax is lowered by
Non competitive inhibitor
KM is not modified by the presence of
Non competitive inhibitor
Affinity of enzyme to the substrate is decreased by the presence of
Competitive inhibitor
The inhibitor and the substrate bind at different sites on the enzyme
Non competitive inhibition
Malathion’s (organophosphate) effect on acetylcholinesterase
Non competitive inhibition
Simvastatin’s effect on HMG-CoA reductase
Competitive inhibition
What is the formula for standard free energy
delta G = delta H - T delta S
Measure of energy available to do work
delta G or Gibbs free energy
Measure of randomness
delta S or entRopy
Measure of heat released or absorbed during a reaction
entHalpy
Heat absorbed : endergonic : positive G
Heat released: exergonic : negative G
Free energy change under standard conditions
Standard free energy
Or
delta G *
Two ways in which your cells produce ATP
Oxidative phosphorylation
Substrate level phosphorylation
Endergonic reaction
Positive G- heat is absorbed
Energy of the products is less than the energy of the reactants
Negative G
Exothermic reaction
Releases energy
Spontaneous reaction
Negative G
Rate of formation of products is the same as the rate of formation of the reactants
delta G is 0
Equilibrium