Bio1- enzymes (starch + amylase) Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

What is the 8 step method to the enzymes practical?

A
  1. Place 1 dot of iodine solution into each well of a spotting tile
  2. Fill three test tubes - 2cm ³ starch solution, 2cm ³ amylase solution, 2cm ³ of pH buffer solution
  3. Place test tubes in a water bath at 30°C, leave for 10mins to allow solutions to reach correct temperature.
  4. Combine all three into one test tube and stir using a stirring rod. Return to water bath and start stopwatch.
  5. After 30secs, use stirring rod to transfer 1drop of solution to a well in the spotting tile.
  6. Iodine turns blue-black if starch is present.
  7. Repeat step 5 until iodine remains orange- this is when starch is no longer present (completed reaction).
  8. Repeat whole experiment with different pH buffers.
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What colour does iodine turn if starch is present?

A

Blue-black

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What colour does iodine change to when starch isn’t present?

A

Remains orange

How well did you know this?
1
Not at all
2
3
4
5
Perfectly