bio test #4 Flashcards
What are the two kinds of nucleic acids?
DNA + RNA
What is the structure of DNA?
Double-stranded helix
What is the structure of RNA?
Single-stranded helix
What nucleotide sugar does DNA use?
Deoxyribose
What nucleotide sugar does RNA use?
Ribose
What are the (4) nucleotide bases of DNA?
Adenine, Thymine, Guanine, Cytosine
(A) (T) (G) (C)
What are the (4) nucleotide bases of RNA?
Adenine, Guanine, Cytosine, Uracil
(A) (G) (C) (U)
Where is DNA located?
The Nucleus
Where is RNA located?
The Nucleus, AND Cytosol
What is the general function of DNA?
Make RNA
What is the general function of RNA?
Make protein
Fill in the following: Genes –> ___ –> ___.
genes –> proteins –> traits
What are genes made of?
DNA
DNA’s structure is a ___ of ___.
Polymer of Nucleotides
DNA’s strands are ____.
(parallel, anti parallel)
Anti parallel
Hydrogen bonds (between the DNA’s structure) can be in groups of ___.
2 or 3
The hydrogen bonds (between the DNA’s structure) are created by ___.
Complimentary base pairings
A nucleotide consists of what (3) things?
Sugar molecule, phosphate, nitrogenous base.
Each strand has a ___ to help with the structural framework of DNA.
Sugar phosphate backbone
Which of the nucleotide bases are Purines?
(A) (T) (G) (C) (U)
(A) (G)
Which of the nucleotide bases are Pyrimidines?
(A) (T) (G) (C) (U)
(C) (U) (T)
[Complimentary Base Pairing Rules]
Adenine (A), only pairs with ___ or ___.
Thymine (T) or Uracil (U)
[Complimentary Base Pairing Rules]
Guanine (G), only pairs with ___.
Cytosine (C)
When going from Chromatin –> Chromosome, it becomes ___.
Condensed
DNA molecules first wrap around the histone proteins forming beads on string structure called ___.
Nucleosomes
___ are proteins that bind to DNA and help give chromosomes their shape.
Histones
DNA replication consists of what (3) steps?
Initiation –> Elongation –> Termination
(DNA replication)
What is the primary enzyme that plays a role in Initiation?
DNA helicase
(DNA replication)
What is the primary enzyme that plays a role in Elongation?
DNA polymerase
(DNA replication)
What is the primary enzyme that plays a role in Termination?
DNA ligase
(DNA replication)
What is the primary function of Initiation?
It unwinds the double-stranded DNA helix.
(DNA replication)
What is the primary function of Elongation?
It adds DNA nucleotides to the DNA template.
(DNA replication)
What is the primary function of Termination?
It seals the gaps between DNA fragments.
Which two strands are complimentary?
Template + Leading strands
What describes the type of [DNA] replication that occurs?
Semi-conservative replication
DNA polymerase can only add DNA in the ___ to ___ direction.
5’ to 3’
What is the central dogma?
Dna makes RNA which makes Proteins
The process through which DNA makes RNA is called ___.
Transcription
The process through which RNA makes Protein is called ___.
Translation
Transcription happens where?
In the Nucleus
Step 1 of RNA processing is to add a 5’ ___ for protection.
Guanine (G) cap
Step 2 of RNA processing is to add a 3’ ___ for protection.
Poly-adenine (AAAA..) tail
Step 3 of RNA processing is to remove ___ and stitch together ___.
REMOVE introns (non-coding sequences)
STITCH exons (coding sequences)
The 3rd step process of removing introns and stitching exons is called ___.
Splicing
After splicing, the strand is ___.
(shorter, longer)
Shorter
Groups of (3) are called ___, which says during translation that it’s time to start making proteins.
Codons
The ___ codon is below the ___ codon.
Start codon is BELOW the Anti codon
___ is always bound to RNA’s anti codon.
Met (Methionine)
The Start codon is always ___.
“AUG”
During the process of translation, it shifts to the ___.
(right, left)
Right –>
What are the (3) sites, in order, called in translation?
[E site] [P site] [A site]
During phase 2 of translation, the amino acid [MET] in the P site forms a bond with [___] in the A site.
GLU
During phase 3 of translation, [MET] and [GLU] are bound together in the P site, while [___] becomes the amino acid in the A site.
SER
What kind of bond is [MET] + [GLU] and [SER] held by?
A peptide bond
Which of the following is NOT true of DNA?
A. Found in the nucleus
B. Double-stranded
C. Uses ribose sugar
D. Uses thymine bases
C. Uses ribose sugar
It does NOT use ribose, it uses deoxyribose
Which of the following bases pairs with adenine?
A. Uracil
B. Thymine
C. Guanine
D. A and B
D. A and B
Which of the following bases pairs with adenine IN RNA?
A. Uracil
B. Thymine
C. Guanine
D. A and B
Uracil
Which of the following is a purine base?
A. Adenine
B. Cytosine
C. Uracil
D. Thymine
Adenine
Nucleosomes are madee of ___.
A. A base, sugar, and phosphate
B. DNA and chromatin
C. DNA and histones
D. RNA and histones
C. DNA and histones
Describe the amount of adenine in a chromosome compared to thymine.
(more, less, same)
SAME
Describe the amount of adenine in a molecule of DNA compared to guanine.
(more, less, same, cant be determined)
CAN NOT BE DETERMINDED
Describe the amount of adenine in a molecule of RNA compared to Uracil.
(more, less, same, can’t be determined)
CAN NOT BE DETERMINDED
Describe the number of purines in a chromosome of DNA compared to pyrimidines.
(more, less, same)
SAME
What is required during translation?
Ribosomes
Describe the level of condensation in chromatin compared to a chromosome.
(more, less, same)
LESS
Which of the following enzymes is responsible for unwinding the DNA helix during DNA replication?
A. DNA helicase
B. DNA polymerase
C. DNA ligase
D. ATP synthase
DNA helicase
Which of the following describes DNA replication?
A. Conservative
B. Semiconservative
C. Nonconservative
D. All of the above
Semiconservative
DNA polymerase synthesizes new DNA strands in the 3’ to 5’ direction.
(True or False)
FALSE
What enzyme is used to make RNA from DNA?
A. DNA helicase
B. DNA polymerase
C. RNA polymerase
D. ATP synthase
RNA POLYMERASE
What is produced during transcription?
A. DNA
B. RNA
C. Protein
D. Lipids
RNA
What is used as the template (i.e. what is being read) during translation?
A. DNA
B. RNA
C. Protein
D. Lipids
RNA
Where does translation occur within a eukaryotic cell?
A. Nucleus
B. Mitochondria
C. Cytosol
D. Plasma membrane
Cytosol
Which of the following sequences is complementary to the DNA coding strand?
A. DNA template strand
B. mRNA transcript
C. codons
D. polypeptide sequence
DNA template strand
Describe the length of the primary RNA transcript compared to the mature RNA transcript.
(longer, shorter, same length)
longer
Which of the following is removed during RNA splicing?
A. Exons
B. Introns
C. Codons
D. Poly-A tail
Introns
In the absence of telomerase, describe the length of telomeres following DNA replication?
(longer, shorter, same length)
Shorter
The two strands of DNA are held together by ___ bonds.
Hydrogen bonds
Which base pairs with Cytosine?
(G) Guanine
Which base pairs with Adenine in DNA?
(T) Thymine
Which of the following is NOT a part of a RNA nucleotide?
A. A phosphate group
B. A nitrogenous base
C. A ribose sugar
D. A histone protein
A histone protein
Which of the following does NOT occur to the RNA transcript after transcription?
A. Addition of 5’ guanine cap
B. Addition of 3’ poly-a-tail
C. Splicing introns together
D. Transport out of the nucleus
Splicing introns together
If a population of consisting of 10 heterozygous individuals (Aa) and 10 homozygous recessive individuals (aa) reproduces, and no evolution occurs, then what will be q in the offspring generation?
75%
Which mechanism of evolution would result from a natural disaster?
Genetic drift
If p = 0.25, then what is q?
0.75
q + p = ?
1
What does the symbol q represent?
the frequency of recessive allele in a population
What does the symbol p represent?
the frequency of dominant allele in a population
(mechanisms of evolution) ___ is when one allele changes into another.
Mutation
(mechanisms of evolution) ___ is change due to random chance.
Genetic drift
(mechanisms of evolution) ___ is pressure that the environment puts on a population.
natural selection
(mechanisms of evolution) ___ is essentially migration
gene flow
What are the 5 mechanisms of evolution?
Gene flow, natural selection, genetic drift, mutation, non random mating
Evolution is a change in the frequency of a(n) ___ in a ___ over time.
of an ALLELE in a POPULATION over time