Bio Tech

Question 1

Recombinant DNA Tech Process - recombinant plasmid making

Answer 1

Obtain DNA fragment fontaining gene of interest
Obtain plasmid from bacterium
Cut both with same Restriction enzyme
Join the DNA fragment and plasmid with DNA Ligase

Question 2

Recombinant DNA Tech - Tranformed Bacteria making

Answer 2

Introduced recombinant plasmid into bacteria (E. Coli)
Selection by Selective Marker - antibiotics
Transformed bacteria cultured in fermenters
Gene cloning - Plasmid replucate to daughter cells - Divide
Form Colonies
Polypeptides extractes and processed

Question 3

Advantage - insulin produced by revombinant DNA tech

4

Answer 3

Structually same with humans - Not rejected
Low Cost
High Yield
Insulin produced is pure

Question 4

GM - maize plants production

Answer 4

Obtain toxin gene from bacterium
Obtain plasmid with Agrobacterium
Cut them with same restriction enzyme
Join them with DNA ligase
Introduce recombinant plasmid to Agrobacterium
Infect maize cells with Agrobacterium
Adult plant developed
Produce bacterial toxin with toxin gene

Question 5

GM - maize plants production

Answer 5

Obtain toxin gene from bacterium
Obtain plasmid with Agrobacterium
Cut them with same restriction enzyme
Join them with DNA ligase
Introduce recombinant plasmid to Agrobacterium
Infect maize cells with Agrobacterium
Adult plant developed
Produce bacterial toxin with toxin gene

Question 6

Advantage of GMO

4

Answer 6

Large amount Short Time Low Cost
Increase food supplies
Higher nutritional value
Reduce Pollution

Question 7

Potential hazards GMO

5

Answer 7

Superbugs from antibiotic resustance gene

Wild types outcompeted by Gmos - reduce biodiversity Upset Ecological Balance

Allergies

Unknown long term effects on health

Gmo genes tranfered to wild type -Genetic Pollution

Question 8

Plant Cloning - Tissue Culture

Answer 8

Tissue from plant removed amd sterilized
Put in sterile culture medium

Mitotic Cell division - Form Callus - Undifferentiated

To culture medium with Hormones - Promote growth of root and shoot

Differentiate into different cells - plantlet

Question 9

Application - plant cloning ^6

Answer 9

Produce Large no. of plants
Produce disease free plants
Rescue infected plant population
Produced endangered plants
Maintain desirable characteristics
Genetically identical plants for research