Bio Tech Flashcards

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1
Q

Genotype

A

The articulation of the genes controlling a given trait

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2
Q

Phenotype

A

The appearance of the trait

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3
Q

Incomplete dominance

A

Offspring displays a mixture the parental characteristics.

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4
Q

Sex-linked characteristics

A

Genes that are on the X chromosome

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5
Q

Autosomal chromosomes

A

Chromosomes other than the X and Y chromosomes

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6
Q

Any genes on the X chromosome in males are on their own, If the one gene is defective,

A

defects will be expressed

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7
Q

Sickle cell anemia:

A

is an autosomal recessive disease and causes impairment in both homozygous and heterozygous conditions

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8
Q

An individual with blood type A

A

may carry the gene for blood type O

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9
Q

Autosomal dominant diseases

A

are expressed in all bearers of the defective gene

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10
Q

If you are a color blind male:

A

none of your sons will be color-blind

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11
Q

Polymerase Chain Reaction (PCR)

A

The use of heat to denature a strand of DNA, ending up with two strands. A primer, Anneal, is used to signal the starting point for the elongation of the DNA strand by Taq polymerase. Nucleotides are added.

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12
Q

Gel Electrophoresis

A

The process of separating fragments of DNA based on size with the use of electrical charge in Gel.

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13
Q

Reverse Transcriptase

A

Makes cDNA in a test tube. This enzyme makes a DNA strand complementary to RNA molecules used as a template.

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14
Q

rDNA

A

contains a segment of DNA from a foreign source

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15
Q

Restrictive enzymes

A

will dissect DNA at specific nucleotide sequences, are naturally occurring, exist in great variety, and cut the DNA so as to create sticky ends.

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16
Q

Sequencing DNA

A

Can be done using a combo of chemicals that stop synthesis at known points and separate segments of DNA of different length by gel electrophoresis and using DNA probes that have the mirror of the base sequences you are looking for.

17
Q

Vectors

A

are a form of genetic material suitable for transport into living cells and include artificial chromosome

18
Q

Artificial chromosomes

A

contain telomeres, a centromere, and a binding site for DNA polymerase

19
Q

Altering the genome of a cell

A

may cause chromosomal damage, interrupt normal gene expression, cause cancer,
requires the use of a vector to introduce new genetic material,
can be done in the form of artificial chromosomes,
has been done successfully in human clinical trials.

20
Q

Microarrays

A

are a collective of DNA fragments from a specific genome, such as a specialized cell

21
Q

Nuclear probes

A

are used to find a given base sequence within a DNA fragment, rely on the inclination of single-stranded DNA to mate with single strands with matching base sequences, carry either a fluorescent or a radioactive label, and provide a means to visualize a gene.

22
Q

DNA Ligase is important because

A

It induces the union of single-stranded DNA segments with matching base pairs into a double-stranded segment and enables hybridization

23
Q

The sticky ends created by restriction enzymes are important because

A

they provide a means to insert new DNA with matching base pairs into a given DNA segment.

24
Q

Undifferentiated Cells

A

also known as stem cells. These cells are unspecialized cells that have the ability to reproduce indefinitely. Stem cells are considered primitive and when found in a given organ, appear to differentiate into cells found in that organ.

25
Q

Differentiated Cells

A

or grown up cells, are usually highly specialized cells and are fully developed. Their appearance and cellular chemistry are devoted to their particular function.

26
Q

SCNT

A

is used extensively to clone animals. SCNT is a method of developing a cell that behaves like an embryonic stem cell but contains the genetic material derived from an adult cell.

27
Q

Transgenic organisms

A

contain foreign genes inserted through lab techniques.

28
Q

Hematopoietic Stem Cells

A

Cells that give rise to blood cells. These cells derive from bone marrow.

29
Q

DNA is not foolproof because

A

Samples could be altered, and DNA pattern may be found in both the suspect’s DNA and someone’s DNA.