Bio 6 - Genetic Lab Techniques Flashcards
What is Gel Electrophoresis?
To sort and separate PCR products according to size. An electric current is passed through it and the smaller fragments of DNA travel the farthest down.
What are the factors that determine how far the the piece protein travels in a Protein electrophoresis?
Charge of the protein and Size of the protein.
What is a Southern blot?
Using DNA probe on a DNA sample.
What is a Northern blot?
Using a DNA probe to look at an RNA sample.
What is a Western blot?
A protein is the sample and an Antibody is the probe.
What is Southwestern blot?
The sample is DNA-binding proteins, and the probe is Oligonucleotides.
What is an Indirect ELISA?
Use a known antigen to identify the presence of an antibody.
What is a Direct Elisa?
Use a known antibody to identify the presence of an antigen.
What is FISH?
Fluorescent DNA or RNA probe. Fluorescence indicates gene is present.
What is it called when we insert the a gene randomly? when targeted insertion?
Constitutive insertion. When target, it is called Conditional insertion.
What type of test uses a known antigen to discern the presence of an antibody?
ELISA test.
What type of test is performed in order to diagnose chromosomal imbalances?
Karyotyping.
What is PCR?
Polimerase chain reaction. It is a way to implify a specific sequence of DNA. There are three steps to it: Denaturation (heat the DNA so that the two strands separate). Annealing (cool the DNA and add DNA primer). Elongation (the DNA polymerase starts to replicate the DNA).
