Bio 125 Lab Practical 1 Flashcards
Brightfield Microscope
a type of compound microscope that allows light rays to pass directly to the eye w/out being deflected by an intervening opaque plate in the condenser
Resolving Power
ability of the lens to show two adjacent objects as discrete entities
Working Distance
proximity of the slide to the bottom of the objective lens
Refractive Index
the light bending ability of glass, oil, and air
Total Magnification Formula
oculars x objective lens = total magnification
Scanning Power =
40x
(10x4)
Low Power =
100x
(10x10)
High Power=
400x
(10x40)
Oil Immersion =
1000x
(10x100)
Working Distances
scanning= 9-10 mm
low power= 5-8mm
high power= 0.5-0.7mm
oil immersion= 0.13-0.18 mm
Parfocal
an object in view under one objective will still be in view under other objectives
Field of View
area seen through a microscope
Vector
agents that carry a pathogen affecting another species
Tick
aka
Ixodes Species
D= Eukarya
K= Animalia
P= Arthropoda
C=Arachnida
G=Ixodes
S=
Itch Mite
aka
Sarcoptes scabiei
D= Eukarya
K= Animalia
P= Arthropoda
C= Arachnida
G= Sarcoptes
S= scabiei
Flea
aka
Xenospylla
D= Eukarya
K= Animalia
P= Arthropoda
C= Insecta
G= Xenospylla
S=
Human Head or Body Louse
aka
Pediculus humanus capitis or corporis
D= Eukarya
K= Animalia
P= Arthropoda
C= Insecta
G= Pediculus humanus
S= capitis or corporis
Parasite
an organism that attacks and consumes parts of an organism much larger than itself
Host
the organism that harbors a parasite
Loss of Competency
when a parasite becomes so dependent on its host that it can no longer live indendently
Acoelomate
without a body cavity
Tapeworm
D= Eukarya
K= Animalia
P= Platyhelminthes (flatworms)
C= Cestoda (tapeworms)
G= Taenia
S= solium
Dog Tapeworm
aka
Echinococcus granulosus
D= Eukarya
K= Animalia
P= Platyhelminthes
C= Cestoda
G= Echinococcus
S= granulosus
Asian Liver Fluke
aka
Clonorchis sinensis
or
Opisthorchis sinensis
D= Eukarya
K= Animalia
P= Platyhelminthes
C= Trematoda (flukes)
G= Clonorchis
S= sinensis
Giant Intestinal Fluke
aka
Fasciolopsis buski
or
Fasciola hepatica (liver fluke)
D= Eukarya
K= Animalia
P= Platyhelminthes
C= Trematoda
G= Fasciolopsis or Fasciola
S= buski or hepatica
Blood Fluke
aka
Schistosoma mansoni
D= Eukarya
K= Animalia
P= Platyhelminthes
C= Trematoda
G= Schistosoma
S= mansoni
Pinworm
aka
Enterobius vermicularis
D= Eukarya
K= Animalia
P= Nemahelminthes or Nematoda (roundworms)
C= Rhabditea
G= Enterobius
S= vermicularis
Intestinal Roundworm
aka
Ascaris lumbricoides
D= Eukarya
K= Animalia
P= Nemahelminthes or Nematoda
C= Rhabditea
G= Ascaris
S= lumbricoides
Hookworm
aka
Ancylostoma caninum
D= Eukarya
K= Animalia
P= Nemahelminthes or Nematoda
C= Rhabditea
G= Ancylostoma
S= caninum
Threadworm
aka
Trichinella spiralis
D= Eukarya
K= Animalia
P= Nematoda
C= Enoplea
G= Trichinella
S= spiralis
Whipworm
aka
Trichuris trichiura
D= Eukarya
K= Animalia
P= Nematoda
C= Enoplea
G= Trichuris
S= trichiura
Leech
aka
Haemopsis species
D= Eukarya
K= Animalia
P= Annelida
C= Hirudinea
G= Haemopsis
S=
Protozoa
unicellular, eukaryotic organisms
Key Features:
- absences of a cell wall
- pellicle: flexible layer outside of the cell membrane
- ability to move by locomotor organelles or gliding mechanism
- mostly heterotrophic but can be photosynthetic
- primarily asexual reproduce thru sexual modes
Amoeba
D= Eukarya
K= n/a
P= Amoebozoa or Rhizopoda
G= Entamoeba
S= histolytica
Amoeba Proteus
D= Eukarya
K= n/a
P= Tubulinea
G= Amoeba
S= proteus
Trypanosoma
D= Eukarya
K= n/a
P= Euglenozoa
G= Trypanosoma
S= lewisi
Giardia
D= Eukarya
K= n/a
P= Metamonada
G= Giardia
S= lamblia
Name the three species of Trichomonas and their locationa
1) Trichomomonas hominis- large intestine, harmless infection
2) Trichomonas tenax- mouth, also harmless
3) Trichomonas vaginalis- vagina, urethra, seminal vesicles; causes venereal disease
Trichomonas
D= Eukarya
K= n/a
P= Metamonada
G= Trichomonas
S= vaginalis
Balantidium
D= Eukarya
K= n/a
P= Ciliophora
G= Balantidium
S= coli
Plasmodium (malaria)
D= Eukarya
K= n/a
P= Apicomplexa
G= Plasmodium
S= malariae
Diatoms aka Plankton
phytoplankton, food chains in the ocean
P= heterokontophytos
Dinoflagellates
- have photosynthetic pigments and are strengthened by internal plates of cellulose
- bioluminescent= red tides
- neurotoxins= can poison humans who eat shellfish that have ingested planktonic dinoflagellates and concentrated their toxins
Fungi
eukaryotic cells which lack chlorophyll (cannot generate energy thru photosynthesis)
- require aerobic environments
- produce enzymes
- absorb nutrients
Saprophytic
obtain nutrients from dead matter
aka parasitic
Distinguishing Features of Fungi
1) eukaryotic
2) heterotrophic
3) lack tissue differentiation
4) chitin cell walls
5) propogate by spores
Fungi belong to Kingdom Myceteae
Name the three divisions
1) Mastigomycota= water molds
2) Gymnomycota= slime molds
3) Amastigomycota= yeasts, molds, bracket fungi, others
Dimorphic
species of fungi that can exist as molds or yeasts under certain conditions
Hyphae
microscopic filaments of molds
Mycelium
mass of intertwined hyphae
Septa
crosswalls that seperate hyphae into individual compartments with an organized nucleus and organelles
Nonseptate or Coenocytic
having no septate
Name the two types of asexual spores seen in molds
1) sporangiospores-form w/in a sac called a sporangium
2) conidia (aka phialospores)- form in specialized hyphae called conidiophores or phialide
- micro and macro conidia
Name the three types of sexual spores seen in molds
1) zygospores- formed from union of nuclear material from hyphae of two diff. strains
2) ascospores- produced in enclosures called asci
3) basidiospores- produced in club shaped bodies called basidia
Yeasts
- don’t have true hyphae
- form multicellular structures called pseudohyphae
- produce asexual spores called blastospores or buds
Ubiquitous
state or capacity of being everywhere at the same time
Chemically defined medium
a medium whose exact chemical composition is known
Agar
an extract from marine red algae
inoculated
intentionally introduced
Colony
a population of cells that theoretically arises from a single bacterial cell
Colony forming unit
a colony that arises from a group of microbes attached to one another
Sterilized
rendered free of life