BB LEMAR NOTES (RH)

Question 1

2ND MOST IMPORTANT BLOOD GROUP SYSTEM IN TERMS OF TRANSFUSION

Answer 1

RH BLOOD GROUP

Question 2

IN RH, IT IS CURRENTLY ACCEPTED THAT 2 CLOSELY LINKED GENES CONTROL THE EXPRESSION OF RH; ________ CODES FOR THE PRESENCE OF RHD AND A SECOND GENE (RHCE) CODES FOR THE EXPRESSION OF _____ ANTIGENS

Answer 2

ONE GENE RHD ; CcEe

Question 3

IN RH, ANTIGENS ARE CHARACTERIZED AS _________________ THE RBC MEMBRANE

Answer 3

NONGLYCOSYLATED PROTEINS IN

Question 4

BASED ON THE THEORY THAT ANTIGEN OF THE SYSTEMS WERE PRODUCED BY 3 CLOSELY LINKED SET OF ALLELES, EACH GENE WAS RESPONSIBLE FOR PRODUCING A PRODUCT (OR ANTIGEN ON THE RED CELLS SURFACE)

Answer 4

FISHER-RACE (DCE)

Question 5

POSTULATED THAT GENE RESPONSIBLE FOR DEFINING RH ACTUALLY PRODUCED AN AGGLUTINOGEN THAT CONTAINED A SERIES OF BLOOD FACTORS, IN WHICH EACH FACTOR IS AN ANTIGEN RECOGNIZED BY AN ANTIBODY

Answer 5

WIENER (Rh-Hr)

Question 6

NUMBER IS ASSIGNED TO EACH ANTIGEN OF THE RH SYSTEM IN ORDER OF ITS DISCOVERY

Answer 6

ROSENFIELD (ALPHA NUMERIC)

Question 7

ADOPTED A 6 DIGIT # FOR EACH BLOOD GROUP SPECIFICITY

Answer 7

ISBT: NUMERIC TERMINOLOGY

Question 8

INTERNATIONAL SOCIETY OF BLOOD TRANSFUSION (ISBT): NUMERIC TERMINOLOGY’S FIRST 3 NUMBERS REPRESENT THE _______ AND THE REMAINING 3 REPRESENT THE ________

Answer 8

SYSTEM ; ANTIGENIC SPECIFICITY

Question 9

D ANTIGENS EXPRESSED APPEAR TO BE COMPLETE, BUT FEW IN #

Answer 9

GENETIC WEAK D

Question 10

1 OR MORE PARTS OF THE D ANTIGEN IS MISSING

Answer 10

D MOSAIC OR PARTIAL D

Question 11

POSITION EFFECT OR GENE INTERACTION EFFECT

Answer 11

C TRANS

Question 12

ALLELE CARRYING D IS TRANS (OPPOSITE HAPLOTYPE) TO THE ALLELE CARRYING C

Answer 12

C TRANS

Question 13

MOST ANTIBODIES ARE IGG AND REACTS OPTIMALLY AT 37degC OR AFTER ANTIGLOBULIN TESTING

IMMUNE ANTIBODIES

DO NOT BIND COMPLEMENTS

RBC DESTRUCTION IS EXTRAVASCULAR

CAUSE HDN

Answer 13

RH ANTIBODIES

Question 14

• MOST COMMON
• CANT BE DIAGNOSED
• CAN AFFECT FIRST CHILD
• WEAK TO NEG DAT
• OCCURS IN TYPE O MOTHERS
• SLIGHT RISE IN BILIRUBIN (TREAT WITH PHOTOTHERAPY)

Answer 14

ABO HDN

Question 15

• SEVERE
• FOLLOWED WITH TITERS
• IMMUNE EXPOSURE (2ND CHILD)
• VERY STRONG DAT
• CAN AFFECT ANY RH-NEG MOTHER
• HIGH RISH IN BILIRUBIN (MAY NEED TO PERFORM EXCHANGE TRANSFUSION)

Answer 15

RH HDN

Question 16

Most common HDN

Answer 16

ABO HDN

Question 17

SEVERE HDN

Answer 17

RH HDN

Question 18

TYPE OF HDN THAT CANT BE DIAGNOSED

Answer 18

ABO HDN

Question 19

HDN FOLLOWED WITH TITERS

Answer 19

RH HDN

Question 20

HDN THAT CAN AFFECT 1ST CHILD

Answer 20

ABO HDN

Question 21

HDH THAT PRODUCE IMMUNE EXPOSURE TO 2ND CHILD

Answer 21

RH HDN

Question 22

HDN WITH WEAK TO NEG DAT

Answer 22

ABO HDN

Question 23

HDN WITH VERY STRONG DAT

Answer 23

RH HDN

Question 24

ABO HDN OCCURS IN GROUP _____ MOMS

Answer 24

TYPE O

Question 25

RH HDN CAN AFFECT ANY ______ MOTHER

Answer 25

RH-NEG

Question 26

SLIGHT RISE IN BILIRUBIN IN ABO HDN CAN BE TREATED WITH ______

Answer 26

PHOTOTHERAPY

Question 27

HIGH RISE IN BILIRUBIN IN RH HDN MAY NEED TO PERFORM _______

Answer 27

EXCHANGE TRANSFUSION

Question 28

SOLUTION OF CONCENTRATED ANTI-RHO(D)

Answer 28

RHO(D) IMMUNE GLOBULIN

Question 29

IT IS PREPARED FROM POOLED HUMAN PLASMA OF PATIENTS WHO HAVE BEEN HYPERIMMUNIZED AND CONTAINS PREDOMINANTLY IGG ANTI-D

Answer 29

RHO(D) IMMUNE GLOBULIN

Question 30

RhIg 2 PRIMARY USES

Answer 30

TREATMENT OF ITP ; PREVENTION OF RH HDN

Question 31

IF THE SCREENING TEST IS NEGATIVE FOR THE PRESENCE OF D+ RBCS OF FETAL ORIGIN, THE MOTHER SHOULD RECEIVE A FULL DOSE OF RHIG WITHIN ______ OF DELIVERY

Answer 31

72 HOURS

Question 32

IF THE SSCREENING TEST IS POSITIVE IN RHIG, THE FMH MUST BE QUANTIFIED USING THE _______

Answer 32

KLEIHAUER-BETKE TEST

Question 33

_________ OR _________ IS USED TO QUANTITATE THE NUMBER OF FETAL RH-POSITIVE CELLS IN THE MOMS CIRCULATION AS A RESULT OF FETOMATERNAL HEMORRHAGE

Answer 33

KLEIHAUR BETKE TEST OR FLOW CYTOMETRY

Question 34

BASED ON THE RESISTANCE OF FETAL HEMOGLOBIN TO ACID TREATMENT

Answer 34

KLEIHAUER BETKE TEST

Question 35

HOW IS KLEIHAUER BETKE TEST IS DONE?

Answer 35

USE: MATERNAL BLOOD 1. THIN SMEAR IN SLIDE 2. TREAT WITH ACID 3. RINSE 4. COUNTERSTAIN 5. READ MICROSCOPICALLY

Question 36

IN READING THE SMEAR, MATERNAL CELLS WILL APPEAR ______ AND THE FETAL CELLS WILL BE ______

Answer 36

GHOSTS ; PINK

Question 37

FETAL CELLS AND METERNAL CELLS ARE COUNTED SEPARATELY FOR A TOTAL OF ______ CELLS

Answer 37

2000 CELLS

Question 38

IF 2K ERYTHROCYTES ARE COUNTED, THE % OF FETAL CELLS IS MULTIPLIED BY ___ TO ESTIMATE THE AMOUNT OF FETAL WHOLE BLOOD PRESENT IN THE MATERNAL CIRCULATION

Answer 38

50