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MICROPARA (DOC) > BASIC LAB EQUIPMENTS > Flashcards

BASIC LAB EQUIPMENTS Flashcards

1
Q

An instrument that produces enlarged images of small objects, allowing an exceedingly close view of minute structures at a scale convenient for examination & analysis

A

Microscope

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2
Q

A type of microscope that uses a single lens to view objects

A

simple microscope

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3
Q

A type of microscope that uses light to view objects and is composed of two lens systems

A

Compound Microscope

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4
Q

Types of compound microscope

A
  1. Bright-field Microscope
  2. Flouresence Microscope
  3. Dark-field Microscope
  4. Phase-contrast Microscope
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5
Q

A most commonly used type of compound microscope in routine microscopy that produces a dark image on a bright background

A

Bright-field Microscope

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6
Q

A type of compound microscope that uses transmitted light to observe targets at high magnification

A

Bright-field Microscope

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7
Q

A brightfield microscope(compound) that is arranged so that the light source is blocked off, causing light to scatter as it hits the specimen

A

Dark-field Microscope

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8
Q

A type of compound microscope that produces a bright image on a dark background

A

Dark-field Microscope

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9
Q

A biological microscope(compound) that observes fluorescence emitted by samples by using special light sources such as mercury lamps

A

Flouresence Microscope

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10
Q

A type of compound microscope that allows coupling compounds such as fluorescein to bind to certain cellular components

A

Flouresence Microscope

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11
Q

Flouresence Microscope allows coupling compounds such as _______ to bind to certain cellular components

A

fluorescein

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12
Q

A type of compound microscope that visualizes minute surface irregularities by using light interference

A

Phase-contrast Microscope

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13
Q

A type of compound microscope that is commonly used to observe living cells without staining them

A

Phase-contrast Microscope

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14
Q

Contains mirrors and allows the body tube to rotate 360°.

A

Rotating Head

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15
Q

_____ produce most of the magnification. The high-power lens (blue stripe) magnifies 40x and the low-power lens (yellow stripe) magnifies 10x.

A

objective lens

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16
Q

Holds the slide and contains an opening that allows light to pass through the specimen on its way to the objective.

A

Stage

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17
Q

Rotating dial that controls the passage of light through the stage. Numbers on the dial indicate the relative amount of light passing, with “5” being the most and “1” being the least.

A

Diaphragm

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18
Q

Supports the microscope and contains the electronics. The microscope should be carried with one hand under the base and the other holding the arm.

A

Base

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19
Q

Turns the light on and off

A

Power switch

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20
Q

Illuminates the specimen by shining bright light through it.

A

light source

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21
Q

Used to make small focus adjustments, esp. when using the high power objective.

A

Fine focus knob

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22
Q

Moves the stage up and down quickly. Used to find a specimen when using the low power objective.

A

Coarse focus knob

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23
Q

Supports the lenses, mirrors, and body tube. The microscope should be carried with one hand holding the ____ and the other under the base.

A

Arm

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24
Q

The _____ is where one views the enlarged object. It contains a lens called the ocular that further magnifies the specimen by 10x

A

eyepiece

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25
Q

Passes light from the head to the eyepiece.

A

body tube

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26
Q

ability of a microscope to produce an image of an object at a scale larger than its actual size

A

Magnification

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27
Q

total magnification is a combination of the _____ and the ______

A

eyepieces and objective lens

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28
Q

Ocular lens magnification = ___x

A

10x

29
Q

Objective Lenses magnification
A.scanning = ____x
B.low power field = ____x
C.high power field = ____x
D.Oil immersion = ____x

A

A.scanning = 4x
B.low power field = 10x
C.high power field = 40x
D.Oil immersion = 100x

30
Q

ability of a microscope to distinguish details of a specimen or sample

A

Resolution / Resolving Power

31
Q

ability to gather light and to resolve fine specimen detail while working at a fixed object (or specimen) distance

A

Numerical Aperture

32
Q

ability of a microscope to stay relatively in focus as the user switches among the objectives

A

Parfocal

33
Q

a shallow transparent covered dish used to culture cells, such as bacteria or fungi

A

Petri Dish

34
Q

Petri dish is also called as:

A

Petri plate or Cell culture dish

35
Q

Petri Dish is named after its inventor, German bacteriologist, _______

A

Julius Richard Petri

36
Q

can hold, mix, or heat contents

A

Culture tubes

37
Q

transparent body allow researchers to constantly monitor content

A

Culture tubes

38
Q

shaped for easier retrieval and minimal sample loss

A

Culture tubes

39
Q

extremely resistant to accommodate any operation or harsh sample

A

Culture tubes

40
Q

also called a smear loop, inoculation wand, microstreaker, wire loop

A

Inoculating Loop

41
Q

consists of a thin handle with a loop about 5 mm wide or smaller at the end

A

Inoculating Loop

42
Q

used to pick up and transfer a small sample (inoculum) from a culture of microorganisms into a petri dish

A

Inoculating Loop

43
Q

used to transfer and inoculate living microorganisms

A

Inoculating Needle

44
Q

made from nichrome or platinum wire affixed to a metallic handle

A

Inoculating Needle

45
Q

base of the Inoculating Needle is dulled, resulting in a _____

A

blunted end

46
Q

Inoculating loop is for ____, while inoculating needle is for _____

A

loop= streaking;
needle= stabbing

47
Q

are used when fingers are too large to grasp small objects and when many objects need to be held at one time

A

Forceps

48
Q

in Bacteriology lab,used for handling glass slides during the staining process

A

Forceps

49
Q

holds multiple microscopic slides simultaneously for staining process

A

Staining Rack

50
Q

used for steaming or heating process tubes, flasks or beakers

A

Water Bath

51
Q

used to hold specimen viewed under the microscope

A

Glass slide/Cover Slips

52
Q

device used to measure the distance between two opposite sides of an object (usually diameter)

A

Vernier Caliper

53
Q

In antibiotic susceptibility testing: it is used to measure the zone of inhibition

A

Vernier Caliper

54
Q

_____ will consume most of O2 in the jar & will produce an elevated level of CO2

A

candle flame

55
Q

these conditions are ideal for growth of microaerophilic organisms

A

elevated level of CO2

56
Q

Filter paper disks impregnated with standardized concentration of an antimicrobial agent

A

antibiotic discs

57
Q

_____ are used in susceptibility testing

A

Antibiotic disc

58
Q

used to grow microorganism in optimum temperatures

A

Incubator

59
Q

maintains a constant temperature specifically suitable for growth of a specific microbe

A

Incubator

60
Q

usual temperature of incubation is ____°C

A

35-37°C

61
Q

used to sterilize liquid substances such as prepared media, solutions, glasswares

A

autoclave

62
Q

water is boiled in a closed container to reach ____°C

A

100°C

63
Q

____ builds up inside the autoclave increasing the temperature much beyond 100°C

A

steam

64
Q

temperature in autoclave reaches _____

A

121°C

65
Q

“Steam under pressure”

A

Autoclave

66
Q

pressure in autoclave reaches ____ pounds/in2 or _____ psi

A

15 pounds/in2 or 15 psi

67
Q

In autoclave, _____ is required to kill all bacteria including heat resistant spore-formers

A

high temperature

68
Q

In autoclave, high temperature is required to kill all bacteria including _________

A

heat resistant spore-formers

MICROPARA (DOC) (9 decks)

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