BASIC LAB EQUIPMENTS

Question 1

An instrument that produces enlarged images of small objects, allowing an exceedingly close view of minute structures at a scale convenient for examination & analysis

Answer 1

Microscope

Question 2

A type of microscope that uses a single lens to view objects

Answer 2

simple microscope

Question 3

A type of microscope that uses light to view objects and is composed of two lens systems

Answer 3

Compound Microscope

Question 4

Types of compound microscope

Answer 4

1. Bright-field Microscope
2. Flouresence Microscope
3. Dark-field Microscope
4. Phase-contrast Microscope

Question 5

A most commonly used type of compound microscope in routine microscopy that produces a dark image on a bright background

Answer 5

Bright-field Microscope

Question 6

A type of compound microscope that uses transmitted light to observe targets at high magnification

Answer 6

Bright-field Microscope

Question 7

A brightfield microscope(compound) that is arranged so that the light source is blocked off, causing light to scatter as it hits the specimen

Answer 7

Dark-field Microscope

Question 8

A type of compound microscope that produces a bright image on a dark background

Answer 8

Dark-field Microscope

Question 9

A biological microscope(compound) that observes fluorescence emitted by samples by using special light sources such as mercury lamps

Answer 9

Flouresence Microscope

Question 10

A type of compound microscope that allows coupling compounds such as fluorescein to bind to certain cellular components

Answer 10

Flouresence Microscope

Question 11

Flouresence Microscope allows coupling compounds such as _______ to bind to certain cellular components

Answer 11

fluorescein

Question 12

A type of compound microscope that visualizes minute surface irregularities by using light interference

Answer 12

Phase-contrast Microscope

Question 13

A type of compound microscope that is commonly used to observe living cells without staining them

Answer 13

Phase-contrast Microscope

Question 14

Contains mirrors and allows the body tube to rotate 360°.

Answer 14

Rotating Head

Question 15

_____ produce most of the magnification. The high-power lens (blue stripe) magnifies 40x and the low-power lens (yellow stripe) magnifies 10x.

Answer 15

objective lens

Question 16

Holds the slide and contains an opening that allows light to pass through the specimen on its way to the objective.

Answer 16

Stage

Question 17

Rotating dial that controls the passage of light through the stage. Numbers on the dial indicate the relative amount of light passing, with “5” being the most and “1” being the least.

Answer 17

Diaphragm

Question 18

Supports the microscope and contains the electronics. The microscope should be carried with one hand under the base and the other holding the arm.

Answer 18

Base

Question 19

Turns the light on and off

Answer 19

Power switch

Question 20

Illuminates the specimen by shining bright light through it.

Answer 20

light source

Question 21

Used to make small focus adjustments, esp. when using the high power objective.

Answer 21

Fine focus knob

Question 22

Moves the stage up and down quickly. Used to find a specimen when using the low power objective.

Answer 22

Coarse focus knob

Question 23

Supports the lenses, mirrors, and body tube. The microscope should be carried with one hand holding the ____ and the other under the base.

Answer 23

Arm

Question 24

The _____ is where one views the enlarged object. It contains a lens called the ocular that further magnifies the specimen by 10x

Answer 24

eyepiece

Question 25

Passes light from the head to the eyepiece.

Answer 25

body tube

Question 26

ability of a microscope to produce an image of an object at a scale larger than its actual size

Answer 26

Magnification

Question 27

total magnification is a combination of the _____ and the ______

Answer 27

eyepieces and objective lens

Question 28

Ocular lens magnification = ___x

Answer 28

10x

Question 29

Objective Lenses magnification A.scanning = ____x B.low power field = ____x C.high power field = ____x D.Oil immersion = ____x

Answer 29

A.scanning = 4x B.low power field = 10x C.high power field = 40x D.Oil immersion = 100x

Question 30

ability of a microscope to distinguish details of a specimen or sample

Answer 30

Resolution / Resolving Power

Question 31

ability to gather light and to resolve fine specimen detail while working at a fixed object (or specimen) distance

Answer 31

Numerical Aperture

Question 32

ability of a microscope to stay relatively in focus as the user switches among the objectives

Answer 32

Parfocal

Question 33

a shallow transparent covered dish used to culture cells, such as bacteria or fungi

Answer 33

Petri Dish

Question 34

Petri dish is also called as:

Answer 34

Petri plate or Cell culture dish

Question 35

Petri Dish is named after its inventor, German bacteriologist, _______

Answer 35

Julius  Richard Petri

Question 36

can hold, mix, or heat contents

Answer 36

Culture tubes

Question 37

transparent body allow researchers to constantly monitor content

Answer 37

Culture tubes

Question 38

shaped for easier retrieval and minimal sample loss

Answer 38

Culture tubes

Question 39

extremely resistant to accommodate any operation or harsh sample

Answer 39

Culture tubes

Question 40

also called a smear loop, inoculation wand, microstreaker, wire loop

Answer 40

Inoculating Loop

Question 41

consists of a thin handle with a loop about 5 mm wide or smaller at the end

Answer 41

Inoculating Loop

Question 42

used to pick up and transfer a small sample (inoculum) from a culture of microorganisms into a petri dish

Answer 42

Inoculating Loop

Question 43

used to transfer and inoculate living microorganisms

Answer 43

Inoculating Needle

Question 44

made from nichrome or platinum wire affixed to a metallic  handle

Answer 44

Inoculating Needle

Question 45

base of the Inoculating Needle is dulled, resulting in a _____

Answer 45

blunted end

Question 46

Inoculating loop is for ____, while inoculating needle is for _____

Answer 46

loop= streaking; needle= stabbing

Question 47

are used when fingers are too large to grasp small objects and when many objects need to be held at one time

Answer 47

Forceps

Question 48

in Bacteriology lab,used for handling glass slides during the staining process

Answer 48

Forceps

Question 49

holds multiple microscopic slides simultaneously  for staining process

Answer 49

Staining Rack

Question 50

used for steaming or heating process tubes, flasks or beakers

Answer 50

Water Bath

Question 51

used to hold specimen viewed under the microscope

Answer 51

Glass slide/Cover Slips

Question 52

device used to measure the distance between two opposite sides of an object (usually diameter)

Answer 52

Vernier Caliper

Question 53

In antibiotic susceptibility testing: it is used to measure the zone of inhibition

Answer 53

Vernier Caliper

Question 54

_____ will consume most of O2 in the jar & will produce an elevated  level of CO2

Answer 54

candle flame

Question 55

these conditions are ideal for growth of microaerophilic organisms

Answer 55

elevated  level of CO2

Question 56

Filter paper disks impregnated with standardized concentration of an antimicrobial agent

Answer 56

antibiotic discs

Question 57

_____ are used in susceptibility  testing

Answer 57

Antibiotic disc

Question 58

used to grow microorganism in optimum temperatures

Answer 58

Incubator

Question 59

maintains a constant temperature specifically suitable for growth of a specific microbe

Answer 59

Incubator

Question 60

usual temperature of incubation is ____°C

Answer 60

35-37°C

Question 61

used to sterilize liquid substances such as prepared media, solutions, glasswares

Answer 61

autoclave

Question 62

water is boiled in a closed container to reach ____°C

Answer 62

100°C

Question 63

____ builds up inside the autoclave increasing the temperature much beyond 100°C

Answer 63

steam

Question 64

temperature in autoclave reaches _____

Answer 64

121°C

Question 65

“Steam under pressure”

Answer 65

Autoclave

Question 66

pressure in autoclave reaches ____ pounds/in2 or _____ psi

Answer 66

15 pounds/in2 or 15 psi

Question 67

In autoclave, _____ is required to kill all bacteria including heat resistant spore-formers

Answer 67

high temperature

Question 68

In autoclave, high temperature is required to kill all bacteria including _________

Answer 68

heat resistant spore-formers