BASIC CONCEPTS ON LABORATORY BIOSAFETY & BIOSECURITY PART 2

Question 1

what classification of fires are ordinary combustible solid materials, such as paper, wood, plastic, and fabric

Answer 1

Class A

Question 2

what classification of fires are combustible/reactive metals, such as
magnesium, sodium, and potassium

Answer 2

Class D

Question 3

what classification of fires are flammable liquids/gases and combustible petroleum
products

Answer 3

Class B

Question 4

what classifications of fire energized electrical equipment

Answer 4

Class C

Question 5

fire or explosion, asphyxiation, pressure buildup, embrittlement of materials, and tissue damage similar to that of thermal
burns

Answer 5

Cryogenic Materials Hazards

Question 6

Liquid nitrogen

Answer 6

Cryogenic Materials Hazards

Question 7

what is laboratory glassware

Answer 7

Glass Pipets

Question 8

should be handled with extra care, as should sharp instruments such as cork borers, needles, scalpel blades,
and other tools

Answer 8

Glass Pipets

Question 9

These physical actions can, over time, contribute to repetitive strain disorders

Answer 9

Ergonomic Hazards

Question 9

These physical actions can, over time, contribute to repetitive strain disorders

Answer 9

Ergonomic Hazards

Question 10

Pressurized water extinguishers, as well as foam and multipurpose dry-chemical types are used for

Answer 10

Class A Fires

Question 11

Multipurpose dry-chemical and carbon dioxide extinguishers are used for

Answer 11

Class B and Class C fires

Question 12

recommended for use with computer equipment

Answer 12

Halogenated hydrocarbon extinguishers

Question 13

What classification of fires present special problems, and extinguishment is left to trained firefighters using special dry-chemical extinguishers

Answer 13

Class D

Question 14

what primary contributing factors associated with repetitive strain disorders
position/posture, applied force, and frequency of repetition

Answer 14

position/posture, applied force, and frequency of repetition

Question 15

may indicate the onset of repetitive strain disorders

Answer 15

Chronic symptoms of pain, numbness, or tingling in extremities

Question 16

should be neutralized before disposal

Answer 16

strong acids or bases

Question 17

should never be disposed of down
the drain

Answer 17

foul-smelling chemicals

Question 18

must be collected in approved
containers and segregated into compatible classes

Answer 18

flammable solvents

Question 19

flush down the drain with copious
quantities of water

Answer 19

water-soluble substances

Question 20

How many Classifications of fires

Answer 20

4 (Class A-D)

Question 21

is a process used to recognize the hazardous characteristics of infectious agents that may be encountered in the clinical
microbiology laboratory

Answer 21

Biological Risk Assessment

Question 22

laboratory practices that could result in an infectious exposure

Answer 22

• the likelihood that a LAI will occur
• the consequences of that infection

Question 23

For an infection to occur: the infectious agent must have a route of transmission to the susceptible host

Answer 23

susceptible host

Question 24

For an infection to occur: must be high enough to cause disease

Answer 24

concentration of the agent

Question 25

two major sources of biological hazards in the microbiology laboratory

Answer 25

- processing of the patient specimens - handling of the actively growing cultures of microorganisms

Question 26

The major routes of LAIs in the clinical laboratory are:

Answer 26

- parenteral inoculations - spills and splashes onto skin or mucous membranes, ingestions - inhalation of infectious aerosols

Question 27

needle sticks or contaminated sharps

Answer 27

parenteral inoculations

Question 28

putting pens or fingers into the mouth, mouth pipetting

Answer 28

spills and splashes onto skin or mucous membranes, ingestions

Question 29

Five steps have been outlined in the CDC’s “Guidelines for Safe Work Practices in Human and Animal Medical Diagnostic Laboratories” for performing a risk assessment.

Answer 29

1. Identify the hazards associated with an infectious agent or material 2. Identify the activities that might cause exposures to the agent or material 3. Consider the competencies and experience of laboratory personnel 4. Evaluate and prioritize risks (evaluate the likelihood that an exposure would cause a LAI and the severity of consequences if such an infection occurs) 5. Develop, implement, and evaluate controls to minimize the risk for exposure

Question 29

Five steps have been outlined in the CDC’s “Guidelines for Safe Work Practices in Human and Animal Medical Diagnostic Laboratories” for performing a risk assessment.

Answer 29

1. Identify the hazards associated with an infectious agent or material 2. Identify the activities that might cause exposures to the agent or material 3. Consider the competencies and experience of laboratory personnel 4. Evaluate and prioritize risks (evaluate the likelihood that an exposure would cause a LAI and the severity of consequences if such an infection occurs) 5. Develop, implement, and evaluate controls to minimize the risk for exposure

Question 30

should be separated from the other parts of the building and be accessed through two self-closing doors

Answer 30

BSL-3 laboratory

Question 31

what personnel must have specific training in handling of these pathogenic and potentially lethal organisms

Answer 31

Laboratory personnel

Question 32

it requires a ducted air ventilation system that must provide for sustained directional air flow

Answer 32

BSL-3 laboratory

Question 33

directional air flow pulls air from

Answer 33

clean area toward “potentially contaminated” areas

Question 34

The ceilings and floors must be solid, and any seams must be sealed

Answer 34

BSL-3 LABORATORY

Question 35

All parts of the laboratory must be constructed for easy cleaning and decontamination

Answer 35

BSL-3 LABORATORY

Question 36

agents that are dangerous and exotic

Answer 36

BIOSAFETY LEVEL 4

Question 37

Agents that are well classified and are not known to cause disease consistently healthy adults

Answer 37

BSL-1 LABORATORY

Question 38

can be transmitted by aerosols, or have an unknown risk of transmission

Answer 38

BIOSAFETY LEVEL 4

Question 39

is located in a separate building or is in an isolated zone within a building

Answer 39

BSL-4 facility

Question 40

Laboratorians should receive immunizations or tests for agents handled or for agents that could potentially be in the laboratory environment

Answer 40

BIOSAFETY LEVEL 2

Question 41

Two types of BSL-4 laboratories

Answer 41

Cabinet laboratory Suit laboratory

Question 41

This facility is isolated from all other areas, and access is strictly controlled.

Answer 41

BSL-4 facility

Question 42

have a high risk of causing life-threatening infections

Answer 42

BIOSAFETY LEVEL 4

Question 43

Infectious agents that require BSL-2 containment and practices are agents that pose a moderate potential hazard for the employees and the environment

Answer 43

Biosafety level 2

Question 44

type of BSL-4 Laboratories that all work is performed within a class III BSC

Answer 44

Cabinet laboratory

Question 45

A biosafety manual must be developed and updated

Answer 45

Biosafety level 2

Question 46

type of BSL-4 Laboratories that personnel wear a positive pressure protective suit to perform all work

Answer 46

Suit laboratory

Question 47

True Or False The BSL-4 laboratory has a dedicated nonrecirculating ventilation system, which is filtered through a HEPA filter before being exhausted

Answer 47

True

Question 48

True or False safety cabinets cannot be used as physical fume hoods.

Answer 48

False ( Chemical Fume hoods)

Question 49

What class uses an exhaust fan to move air inward through the open front

Answer 49

Class I

Question 50

what class that air is pulled inward and downward by a blower and passed up through the airflow plenum where it passes through a HEPA filter before reaching the work surface

Answer 50

Class ll

Question 51

What class is the most common in microbiology laboratories

Answer 51

Class ll

Question 52

What class is a self-contained ventilated system for highly infectious microorganisms or materials and provides the highest level of personal protection

Answer 52

Class lll

Question 53

The objective of biosecurity is to prevent loss, theft or misuse of microorganisms, biological materials, and research-related information

Answer 53

Biosecurity

Question 54

What Elements of a Biosecurity Program is an organizational structure for the biosecurity program

Answer 54

Program Management

Question 55

What Elements of a Biosecurity Program personnel and visitor identification, visitor management, access procedures, and reporting of security incidents

Answer 55

Personal Management

Question 56

What Elements of a Biosecurity Program identifying the roles and responsibilities for employees who handle, use, store and transport dangerous pathogens and/or other important assets

Answer 56

Personal Management

Question 57

What Elements of a Biosecurity Program is the chain of command, roles, and responsibilities methods for limiting access

Answer 57

Program Management

Question 58

the objective is to know what agents exist at a facility, where they are located, and who is responsible for them.

Answer 58

Inventory and Accountability

Question 59

The objective of an information security program is to protect information from unauthorized release and ensure that the appropriate level of confidentiality is preserved

Answer 59

Information Security

Question 60

Transport policies should address the need for appropriate documentation and material accountability and control procedures for pathogens in transit between locations

Answer 60

Transport of Biological Agents

Question 61

the preservation of human life, the safety and health of laboratory employees and the surrounding community

Answer 61

Accident, Injury and Incident Response Plans

Question 62

bomb threats, natural disasters and severe weather, power outages

Answer 62

Accident, Injury and Incident Response Plans

Question 63

a “chain- of-notification” should be established in advance of an actual event.

Answer 63

Reporting and Communication

Question 64

loss or theft of materials, emergency response to accidents and injuries, incident reporting and identification of and response to security breaches

Answer 64

Training and practice drills

Question 65

biosecurity program audits and implement corrective actions as needed

Answer 65

Security Updates and Re-evaluations

Question 66

the intentional use of bacteria, viruses, fungi, or toxins to injure people, animals, or crops to cause civil and economic unrest and can be classified as overt or covert

Answer 66

Bioterrorism

Question 67

the impact will be immediate and there will be early recognition of the event, generally by emergency response personnel

Answer 67

Overt Bioterrorism

Question 68

the recognition, as well as the response could be delayed, allowing the disease to disseminate throughout the population

Answer 68

Covert Bioterrorism

Question 69

the deliberate use of bacteria,viruses, fungi, or toxins to injure people, animals, or crops to gain a military advantage or for political gain

Answer 69

Biological Warfare