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MLSP FINALS > BASIC CONCEPTS ON LABORATORY BIOSAFETY & BIOSECURITY PART 2 > Flashcards

BASIC CONCEPTS ON LABORATORY BIOSAFETY & BIOSECURITY PART 2 Flashcards

1
Q

what classification of fires are ordinary combustible solid materials, such as paper, wood, plastic, and fabric

A

Class A

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2
Q

what classification of fires are combustible/reactive metals, such as
magnesium, sodium, and potassium

A

Class D

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3
Q

what classification of fires are flammable liquids/gases and combustible petroleum
products

A

Class B

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4
Q

what classifications of fire energized electrical equipment

A

Class C

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5
Q

fire or explosion, asphyxiation, pressure buildup, embrittlement of materials, and tissue damage similar to that of thermal
burns

A

Cryogenic Materials Hazards

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6
Q

Liquid nitrogen

A

Cryogenic Materials Hazards

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7
Q

what is laboratory glassware

A

Glass Pipets

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8
Q

should be handled with extra care, as should sharp instruments such as cork borers, needles, scalpel blades,
and other tools

A

Glass Pipets

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9
Q

These physical actions can, over time, contribute to repetitive strain disorders

A

Ergonomic Hazards

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9
Q

These physical actions can, over time, contribute to repetitive strain disorders

A

Ergonomic Hazards

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10
Q

Pressurized water extinguishers, as well as foam and multipurpose dry-chemical types are used for

A

Class A Fires

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11
Q

Multipurpose dry-chemical and carbon dioxide extinguishers are used for

A

Class B and Class C fires

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12
Q

recommended for use with computer equipment

A

Halogenated hydrocarbon extinguishers

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13
Q

What classification of fires present special problems, and extinguishment is left to trained firefighters using special dry-chemical extinguishers

A

Class D

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14
Q

what primary contributing factors associated with repetitive strain disorders
position/posture, applied force, and frequency of repetition

A

position/posture, applied force, and frequency of repetition

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15
Q

may indicate the onset of repetitive strain disorders

A

Chronic symptoms of pain, numbness, or tingling in extremities

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16
Q

should be neutralized before disposal

A

strong acids or bases

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17
Q

should never be disposed of down
the drain

A

foul-smelling chemicals

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18
Q

must be collected in approved
containers and segregated into compatible classes

A

flammable solvents

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19
Q

flush down the drain with copious
quantities of water

A

water-soluble substances

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20
Q

How many Classifications of fires

A

4 (Class A-D)

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21
Q

is a process used to recognize the hazardous characteristics of infectious agents that may be encountered in the clinical
microbiology laboratory

A

Biological Risk Assessment

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22
Q

laboratory practices that could result in an infectious exposure

A
  • the likelihood that a LAI will occur
  • the consequences of that infection
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23
Q

For an infection to occur: the infectious agent must have a route of transmission to the susceptible host

A

susceptible host

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24
Q

For an infection to occur: must be high enough to cause disease

A

concentration of the agent

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25
Q

two major sources of biological hazards in the microbiology laboratory

A
  • processing of the patient specimens
  • handling of the actively growing cultures of
    microorganisms
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26
Q

The major routes of LAIs in the clinical laboratory are:

A
  • parenteral inoculations
  • spills and splashes onto skin or mucous membranes, ingestions
  • inhalation of infectious aerosols
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27
Q

needle sticks or contaminated sharps

A

parenteral inoculations

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28
Q

putting pens or fingers into the mouth, mouth pipetting

A

spills and splashes onto skin or mucous membranes, ingestions

29
Q

Five steps have been outlined in the CDC’s “Guidelines for Safe Work
Practices in Human and Animal Medical Diagnostic Laboratories” for
performing a risk assessment.

A
  1. Identify the hazards associated with an infectious agent or material
  2. Identify the activities that might cause exposures to the agent or material
  3. Consider the competencies and experience of laboratory personnel
  4. Evaluate and prioritize risks (evaluate the likelihood that an exposure would cause a LAI and the severity of consequences if such an infection occurs)
  5. Develop, implement, and evaluate controls to minimize the
    risk for exposure
29
Q

Five steps have been outlined in the CDC’s “Guidelines for Safe Work
Practices in Human and Animal Medical Diagnostic Laboratories” for
performing a risk assessment.

A
  1. Identify the hazards associated with an infectious agent or material
  2. Identify the activities that might cause exposures to the agent or material
  3. Consider the competencies and experience of laboratory personnel
  4. Evaluate and prioritize risks (evaluate the likelihood that an exposure would cause a LAI and the severity of consequences if such an infection occurs)
  5. Develop, implement, and evaluate controls to minimize the
    risk for exposure
30
Q

should be separated from the other parts of the building and be accessed through two self-closing doors

A

BSL-3 laboratory

31
Q

what personnel must have specific training in handling of these pathogenic and potentially lethal organisms

A

Laboratory personnel

32
Q

it requires a ducted air ventilation system that must provide for sustained directional air flow

A

BSL-3 laboratory

33
Q

directional air flow pulls air from

A

clean area toward “potentially contaminated” areas

34
Q

The ceilings and floors must be solid, and any seams must be sealed

A

BSL-3 LABORATORY

35
Q

All parts of the laboratory must be constructed for easy cleaning and decontamination

A

BSL-3 LABORATORY

36
Q

agents that are dangerous and exotic

A

BIOSAFETY LEVEL 4

37
Q

Agents that are well classified and are not known to cause disease consistently healthy adults

A

BSL-1 LABORATORY

38
Q

can be transmitted by aerosols, or have an unknown risk of transmission

A

BIOSAFETY LEVEL 4

39
Q

is located in a separate building or is
in an isolated zone within a building

A

BSL-4 facility

40
Q

Laboratorians should receive immunizations or tests for agents handled or for agents that could potentially be in the laboratory environment

A

BIOSAFETY LEVEL 2

41
Q

Two types of BSL-4 laboratories

A

Cabinet laboratory
Suit laboratory

41
Q

This facility is isolated from all other areas, and access is strictly controlled.

A

BSL-4 facility

42
Q

have a high risk of causing life-threatening infections

A

BIOSAFETY LEVEL 4

43
Q

Infectious agents that require BSL-2 containment and practices are agents that pose a moderate potential hazard
for the employees and the environment

A

Biosafety level 2

44
Q

type of BSL-4 Laboratories that all work is performed within a class III BSC

A

Cabinet laboratory

45
Q

A biosafety manual must be developed and updated

A

Biosafety level 2

46
Q

type of BSL-4 Laboratories that personnel wear a positive pressure protective suit to
perform all work

A

Suit laboratory

47
Q

True Or False

The BSL-4 laboratory has a dedicated nonrecirculating ventilation system, which is filtered through a HEPA filter before being exhausted

A

True

48
Q

True or False

safety cabinets cannot be used as physical fume hoods.

A

False ( Chemical Fume hoods)

49
Q

What class uses an exhaust fan to move air inward through the open front

A

Class I

50
Q

what class that air is pulled inward and downward by a blower and passed up
through the airflow plenum where it passes through a HEPA filter before reaching the work surface

A

Class ll

51
Q

What class is the most common in microbiology laboratories

A

Class ll

52
Q

What class is a self-contained ventilated system for highly infectious
microorganisms or materials and provides the highest level of personal protection

A

Class lll

53
Q

The objective of biosecurity is to prevent loss, theft or misuse of microorganisms, biological materials, and research-related information

A

Biosecurity

54
Q

What Elements of a Biosecurity Program is an organizational structure for the biosecurity program

A

Program Management

55
Q

What Elements of a Biosecurity Program personnel and visitor identification, visitor management, access procedures, and reporting of security incidents

A

Personal Management

56
Q

What Elements of a Biosecurity Program identifying the roles and responsibilities for employees who handle, use, store and transport dangerous pathogens and/or
other important assets

A

Personal Management

57
Q

What Elements of a Biosecurity Program is the chain of command, roles, and responsibilities methods for limiting access

A

Program Management

58
Q

the objective is to know what agents exist at a facility, where they are located, and who is responsible for them.

A

Inventory and Accountability

59
Q

The objective of an information security program is to protect information from unauthorized release and ensure that the
appropriate level of confidentiality is preserved

A

Information Security

60
Q

Transport policies should address the need for appropriate documentation and material accountability and control procedures for pathogens in transit between locations

A

Transport of Biological Agents

61
Q

the preservation of human life, the safety and health of laboratory employees and the surrounding community

A

Accident, Injury and Incident Response Plans

62
Q

bomb threats, natural disasters and severe weather, power outages

A

Accident, Injury and Incident Response Plans

63
Q

a “chain- of-notification” should be established in advance of an actual event.

A

Reporting and Communication

64
Q

loss or theft of materials, emergency response to accidents
and injuries, incident reporting and identification of and
response to security breaches

A

Training and practice drills

65
Q

biosecurity program audits and implement corrective actions as needed

A

Security Updates and Re-evaluations

66
Q

the intentional use of bacteria, viruses, fungi, or toxins to injure people, animals, or crops to cause civil and economic unrest and
can be classified as overt or covert

A

Bioterrorism

67
Q

the impact will be immediate and there will be early recognition of the event, generally by emergency response personnel

A

Overt Bioterrorism

68
Q

the recognition, as well as the response
could be delayed, allowing the disease to disseminate throughout the population

A

Covert Bioterrorism

69
Q

the deliberate use of bacteria,viruses, fungi, or toxins to injure people, animals, or crops to gain a military advantage or for
political gain

A

Biological Warfare

MLSP FINALS (7 decks)

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