BASIC CONCEPTS ON LABORATORY BIOSAFETY & BIOSECURITY PART 1 Flashcards
Potential hazards in Laboratory
- electric shock
- toxic vapors
- compressed gases
- flammable liquids
- radioactive material
- corrosive substances
- mechanical trauma
-poisons - biologic materials
What Association developed a
standard hazard identification system(diamond-shaped,color-coded symbol), which has been adopted by many clinical laboratories
National Fire Protection Association (NFPA)
Signage and Labeling: Health hazard
(blue quadrant)
Signage and Labeling: Flammable hazard
(red quadrant)
Signage and Labeling: Reactivity/stability hazards
(yellow quadrant)
Signage and Labeling: Other special information
(white quadrant)
SAFETY RESPONSIBILITY
Employer’s Responsibilities
- Establish laboratory work methods and safety policies
- Provide supervision and guidance to employees
- Provide safety information, training, PPE, and medical surveillance to employees
- Provide and maintain equipment and laboratory facilities that are free of recognized hazards and adequate for the tasks required
SAFETY RESPONSIBILITY
Employee’s Responsibilities
- Know and comply with the established laboratory safe work practices
- Have a positive attitude toward supervisors, coworkers, facilities, and safety training
- Be alert and give prompt notification of unsafe conditions or practices to the immediate supervisor and ensure that unsafe
conditions and practices are corrected - Engage in the conduct of safe work practices and use of PPE
All in-house prepared reagents and solutions should be labeled in a standard manner and includes:
- chemical identity
- concentration
- hazard warning
- special handling
- storage conditions
- date prepared
- expiration date (if applicable)
- preparer’s initials
how many gallons of water does the safety shower need to deliver
30 to 50 gallons of water per minute at 20 to
50 pounds per square inch (psi)
located in areas where corrosive liquids are stored or used
Safety Shower
must be accessible (i.e., within 100 feet or 10 s travel) in laboratory areas presenting chemical or biological exposure hazards
Eyewash stations
Other items that must be available for personnel include:
fire blankets, spill kits, and first aid supplies
what devices must be used for manipulating all types of liquids in the laboratory, including water
Mechanical pipeting
device that strictly prohibited
Mouth pipeting
designed to offer various levels of protection, depending on the biosafety level of the specific laboratory
BIOSAFETY CABINETS
storage and handling of hazardous chemicals and compressed gases
Chemical Storage Equipment
should always be used to transport glass bottles of acids, alkalis, or organic solvents in volumes larger than 500 mL
Safety carriers
with self-closing doors are required for the storage of flammable liquids
Steel safety cabinets
should be used for storing, dispensing, or disposing of flammables in volumes greater than 1 quart
Approved safety cans
may be used to store flammable materials
Explosion proof refrigerators
Safety glasses, goggles, visors, or work shields protect the eyes and face from splashes and impact
PPE and HYGIENE
If any solution is accidentally splashed into the eye(s), thorough __________ is required
irrigation
protect the hands and arms
when using caustic chemicals
Gloves and rubberized sleeves
