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MLSP FINALS > BASIC CONCEPTS ON LABORATORY BIOSAFETY & BIOSECURITY PART 1 > Flashcards

BASIC CONCEPTS ON LABORATORY BIOSAFETY & BIOSECURITY PART 1 Flashcards

1
Q

Potential hazards in Laboratory

A
  • electric shock
  • toxic vapors
  • compressed gases
  • flammable liquids
  • radioactive material
  • corrosive substances
  • mechanical trauma
    -poisons
  • biologic materials
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2
Q

What Association developed a
standard hazard identification system(diamond-shaped,color-coded symbol), which has been adopted by many clinical laboratories

A

National Fire Protection Association (NFPA)

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3
Q

Signage and Labeling: Health hazard

A

(blue quadrant)

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4
Q

Signage and Labeling: Flammable hazard

A

(red quadrant)

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5
Q

Signage and Labeling: Reactivity/stability hazards

A

(yellow quadrant)

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6
Q

Signage and Labeling: Other special information

A

(white quadrant)

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7
Q

SAFETY RESPONSIBILITY
Employer’s Responsibilities

A
  • Establish laboratory work methods and safety policies
  • Provide supervision and guidance to employees
  • Provide safety information, training, PPE, and medical surveillance to employees
  • Provide and maintain equipment and laboratory facilities that are free of recognized hazards and adequate for the tasks required
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8
Q

SAFETY RESPONSIBILITY
Employee’s Responsibilities

A
  • Know and comply with the established laboratory safe work practices
  • Have a positive attitude toward supervisors, coworkers, facilities, and safety training
  • Be alert and give prompt notification of unsafe conditions or practices to the immediate supervisor and ensure that unsafe
    conditions and practices are corrected
  • Engage in the conduct of safe work practices and use of PPE
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9
Q

All in-house prepared reagents and solutions should be labeled in a standard manner and includes:

A
  • chemical identity
  • concentration
  • hazard warning
  • special handling
  • storage conditions
  • date prepared
  • expiration date (if applicable)
  • preparer’s initials
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10
Q

how many gallons of water does the safety shower need to deliver

A

30 to 50 gallons of water per minute at 20 to
50 pounds per square inch (psi)

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11
Q

located in areas where corrosive liquids are stored or used

A

Safety Shower

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12
Q

must be accessible (i.e., within 100 feet or 10 s travel) in laboratory areas presenting chemical or biological exposure hazards

A

Eyewash stations

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13
Q

Other items that must be available for personnel include:

A

fire blankets, spill kits, and first aid supplies

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14
Q

what devices must be used for manipulating all types of liquids in the laboratory, including water

A

Mechanical pipeting

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15
Q

device that strictly prohibited

A

Mouth pipeting

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16
Q

designed to offer various levels of protection, depending on the biosafety level of the specific laboratory

A

BIOSAFETY CABINETS

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17
Q

storage and handling of hazardous chemicals and compressed gases

A

Chemical Storage Equipment

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18
Q

should always be used to transport glass bottles of acids, alkalis, or organic solvents in volumes larger than 500 mL

A

Safety carriers

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19
Q

with self-closing doors are required for the storage of flammable liquids

A

Steel safety cabinets

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20
Q

should be used for storing, dispensing, or disposing of flammables in volumes greater than 1 quart

A

Approved safety cans

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21
Q

may be used to store flammable materials

A

Explosion proof refrigerators

22
Q

Safety glasses, goggles, visors, or work shields protect the eyes and face from splashes and impact

A

PPE and HYGIENE

23
Q

If any solution is accidentally splashed into the eye(s), thorough __________ is required

A

irrigation

24
Q

protect the hands and arms
when using caustic chemicals

A

Gloves and rubberized sleeves

25
Q

an acceptable alternative for people with latex allergies

A

Polyvinyl or other nonlatex gloves

26
Q

preferably with knit-cuffed sleeves, should be full length and buttoned and made of liquid- resistant material

A

Laboratory coat

27
Q

shoes constructed of porous materials, open-toed shoes, and sandals are considered ineffective against spilled hazardous liquids

A

Proper footwear

28
Q

high-efficiency particulate air (HEPA)
filters must be worn when engineering controls are not feasible

A

Respirators

29
Q

infection control and chemical hygiene

A

Hand Washing

30
Q

after removing gloves, hands should be washed thoroughly with

A

soap and warm water

31
Q

After any work with highly toxic or carcinogenic chemicals, the face should also be

A

washed

32
Q

classified according to flash point, which is the temperature at which sufficient vapor is given off to form an ignitable mixture
with air

A

Flammable/Combustible Chemicals

33
Q

flash point below 37.8°C (100°F)

A

flammable liquid

34
Q

flash point at or above 37.8°C (100°F)

A

combustible liquid

35
Q

what is a major source of safety information for employees who may use hazardous materials in their occupations

A

Safety Data Sheer (SDS)

36
Q

SAFETY DATE SHEETS

A
  • Product name and identification
  • Hazardous ingredients
  • Permissible exposure limit
  • Physical and chemical data
  • Health hazard data and carcinogenic potential
  • Primary routes of entry
  • Fire and explosion hazards
  • Reactivity data
  • Spill and disposal procedures
  • PPE recommendations
  • Handling
    -Emergency and first aid procedures
  • Storage and transportation
    precautions
  • Chemical manufacturer’s name, address, and telephone number
  • Special information section
37
Q

requires each laboratory that uses hazardous chemicals to have a written chemical hygiene plan

A

OSHA LABORATORY STANDARD

38
Q

this plan provides procedures and work practices for regulating and reducing exposure of laboratory personnel
to hazardous chemicals

A

OSHA LABORATORY STANDARD

39
Q

pose a physical or health hazard from
acute or chronic exposure

A

Hazardous chemicals

40
Q

substances that affect cellular development
in a fetus or embryo), carcinogens, and other toxic chemicals

A

teratogens

41
Q

injurious to skin or eyes by direct contact

A

Corrosive Chemicals

42
Q

tissue of the respiratory and gastrointestinal tracts if inhaled or ingested

A

Corrosive Chemicals

43
Q

Examples include in Corrosive Chemicals

A

acids - acetic, sulfuric, nitric, and hydrochloric

bases - ammonium hydroxide, potassium hydroxide, and sodium hydroxide

44
Q

can spontaneously explode or ignite or that evolve heat or flammable or explosive gases

A

Reactive Chemicals

45
Q

some strong acids or bases react with water to generate heat

A

exothermic reactions

46
Q

substances that have been determined to be cancer-causing agents

A

Carcinogenic Chemicals

47
Q

Common example in Carcinogenic Chemicals

A

Benzidine

48
Q

the laboratory must maintain an ________ inventory of carcinogens

A

accurate

49
Q

fire triangle has been modified into three
dimensional pyramid known as the

A

fire tetrahedron

50
Q

cause severe burns and require immediate flushing with copious amounts of _________

A

Clean Water

MLSP FINALS (7 decks)

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