Basic Bacteriology Flashcards
Lipoteichoic acid induces what?
TNF and IL-1
Lipoteichoic acid is from Gram (+)
what is resistant to dehydration, heat and chemicals?
spore
capsules are made of what? and what is the exception?
polysaccharide
exception: Bacillus anthracis is D-glutamate
Spiral, Gram Negatives
boreelia, leptospira, treponema
mycolic acid
mycobacteria
sterols and no cell wall
mycobacteria
2 organisms that are pleomorphic
Rickettsiae & Chlamydiae
Bugs that don’t Gram stain well
Treponema, Mycobacteria, Mycoplasma, Legionella, Rickettsia, Chlamydia
Bugs that Giemsa Stain
Chlamydia, Borrelia, Rickettsiae, Trypanosome, Plasmodium
PAS stain
stains glycogen and mucopolysaccharides: diagnoses Whipple dx
Ziehl-Neelsen stain
acid-fast organisms like Nocardia and Mycobacterium
Mucicarmine stain
Cryptococcus neoformans
india ink
cryptococcus neoformans
silver stain
fungi, legionella, H. pylori
Culture for H. influenza
chocolate agar with factors V (NAD) and X (heme)
Thayer Martin cultures what organisms?
N. gonorrhoeae and N. meningitidis
Substances found in Thayer Martin
Vancomycin to inhibit Gram (+)
Polymyxin to inhibit Gram (-) except neisseria
Nystatin to inhibit fungi
Lowenstein-Jensen Agar
M. tuberculosis
Agar for M. pneumonia
Eaton agar, requires cholesterol
MacConkey agar and what the colony colors mean
Pink colonies - lactose fermenting for ETEC, EIEC, etc
Clear colonies - EHEC, doesn’t ferment lactose
Eosin-methylene blue agar
E. coli - colonies are a green metalic sheen
Agar for Legionella
Charcoal Yeast Extract with cysteine and iron
Sabouraud Dextrose Agar
Fungi
Bug that is reactivated when immune compromised or on TNF-alpha inhibitors, and where it is located
Mycobacterium tuberculosis, apices of the lungs because highest pO2