BACTOLAB 8-11 Flashcards
Köster-positive bacteria are red.
True
We use 5% sulphuric acid for Köster-staining.
False, (we use 0.5% H2SO4)
Brucella melitensis is an obligate aerobic bacterium.
True
Cattle can be infected by Brucella ovis.
False, (sheep)
Brucella abortus is flagellated bacteria.
False, (no flagella)
Pseudomonas aeruginosa produces two types of pigments.
True, pyocyanin and fluorescein
Burkholderia mallei can infect swine.
False, (burkholderia pseudomallei)
Francisella subspecies can be found in rodents and in arthropods.
True
Bordetella bronchiseptica can cause pneumonia in rabbits.
True
Brucella abortus is a facultative pathogenic bacterium.
False, (facultative intracellular bacterium)
We use 3% safranin for Köster-staining.
True
As a result of Köster-staining Brucella abortus is red.
True
Brucella suis is an obligate anaerobic bacterium.
False, (obligate aerobic bacterium)
Brucella suis biotype 2 can infect the European brown hare.
True, and wild boar
Glanders (malleus) is caused by Burkholderia pseudomallei.
False, (by Burkholderia mallei)
Pseudomonas aeruginosa is a flagellated bacterium species.
True, 1 polar flagellum
Burkholder pseudomallei can infect humans.
True
Francisella tularensis needs cysteine or cystine for culturing.
True
Aeromonas spp. is obligate aerobic bacteria.
False, (aerobic and facultative anaerobic)
Brucella species can be found in the infected animals and their direct environment only.
True
Brucella suis has 5 biotypes.
TRUE
- Brucella abortus is a Köster-positive bacterium species.
True
- B. melitensis, B. abortus and B. canis have a strong relationship in antigenic structure, so they show cross reactions.
False, (B. melitensis - B. abortus - B. suis, cross reaction and B. ovis - B. canis, cross reaction)
- Pseudomonas aeruginosa can cause septicaemia in fur animals.
True
- Pseudomonas species are obligate aerobic bacteria.
True
- Humans are susceptible to Burkholderia mallei.
True
- Optimal temperature for culture of Aeromonas hydrophila is above 28°C.
False, (optimal temperature is 28°C)
- Francisella tularensis is the causative agent of tularaemia.
True
- Moraxella ovis – we can see cocci in pairs in the smear.
True
- Brucella species are obligate pathogenic bacteria.
True
- B. suis is a Köster-negative species.
False
- During Köster-staining 3% safranin is used as a counter staining.
True
- B. ovis is a zoonotic agent.
False
- B. mallei is a widespread bacterium in our environment.
False, B. (pseudomallei is widespread
- B. pseudomallei is a flagellated bacterium.
True
- A. salmonicida can cause furunculosis in fish.
True
- Cystine or cysteine is needed as an additive for culturing of F. tularensis
True
- Moraxella ovis can cause pneumonia in calves and sheep.
TRUE
- Brucella sp. is able to multiple in the environment.
False
- Brucella spp. is obligate anaerobic bacteria.
False, obligate aerobic
- Pseudomonas aeruginosa causes otitis externa in rabbits.
False, (in dogs, and sometimes cats)
- B. pseudomallei is not a flagellated bacterium.
False, (have flagella)
Morphology of vibrio species are curved rods, with flagella.
True
Campylobacter jejuni can cause diarrhoea in humans.
True
Lawsonia intracellularis can be cultured on enterocyte cell culture.
True
Helicobacter pylori can cause chronic gastritis and ulcer in humans.
True
Spirochaetes have characteristic morphology, thin helical filaments.
True
Campylobacter species can tolerate 4-6% oxygen.
True, (6%)
Borrelia burgdorferi can cause Lyme-disease in humans.
True
Brachyspira hyodysenteriae is an obligate aerobic bacterium.
False
Human syphilis is caused by Treponema pallidum.
True
Maintenance host of Leptospira sejroe is cattle.
False, (mouse)
Vibrio species can be cultured in an alkaline environment (pH 8.6).
True
Human cholera is caused by Vibrio parahaemolyticus.
False, (vibrio cholera)
Campylobacter species are aerobic, facultative anaerobic bacteria.
False, (microaerophilic)
Campylobacter fetus spp. venerealis can cause abortion in sheep.
False, (cause cattle abortion/infertility)
Swine proliferative enteropathies are caused by Lawsonia intracellularis.
True
Helicobacter species are urease-negative bacteria.
False, (urease positive)
Spirochaetes are non-motile bacteria.
False, (periplasmic flagella)
Borrelia species can be cultured on chocolate agar.
False, (giemsa, fuchin and unstained)
Maintenance host of Leptospira icterohaemorrhagiae is rats.
True
Leptospira hardjo can cause abortion in cattles.
True, (also in sheep)
Causative agent of swine dysentery has 9 serogroups.
True
- B. anserina causes rabbit syphilis.
False, (by T. paralysis cuniculi)
- Leptospira species causes leptospirosis in cattle.
True, (in calves)
- Helicobacter can cause gastritis.
True
- Treponema pallidum is a zoonotic agent.
True
- Helicobacter species are bacteria.
True
- Lyme-disease is caused by Brachyspira.
False, (by Borrelia burgoferi, B. afzelii, B. garinii)
- Brachyspira are obligate anaerobic bacteria.
TRUE
- The causative agent of swine dysentery can show haemolysis on blood agar.
True
- Leptospirosis of dogs is mainly caused by L. grippotyphosa.
False, (leptospirosis interrogans serovar canicola)
- Levaditi ́s silver impregnation is an appropriate staining method for Leptospira species.
True
- Vibrio species are microaerophilic bacteria.
False, (facultative anaerobic)
- For the selective culture of Campylobacter species obligate vancomycin-polymyxin-trimethoprim is used.
True
Infectious form of Chlamydia species is elementary in the body.
True
NAD is needed for culturing Chlamydia species.
False
As a result of Stamp-staining chlamydia is green.
False
Chlamydia psittaci can cause conjunctivitis in birds.
True
Rickettsia species can propagate in lymphoid and endothelial cells only.
False, (Rickettsia, Coxiella: propagate in all cell types) and (Ehrlichia, Neorickettsia: lymphoid cells, endothelial cells)
Q-fever is caused by Coxiella burnetti.
True
Mycoplasmas have a pleomorphic shape.
True, pleomorphic pear shaped
Mycoplasmas are obligate anaerobic bacteria.
False, (facultative anaerobic)
Contagious bovine pleuropneumonia is caused by Mycoplasma bovis.
False, (by M. mycoides ssp. mycoides SC
Mycoplasma gallisepticum can cause chronic respiratory disease in birds.
True
Fuchsin is used as counter staining in the Stamp-staining.
False, (3% diluted malachite-green)
The resistance of the reticulated body is higher than the resistance of the elementary body.
False
Trachoma is caused by Chlamydia psittaci in humans.
False, (by chlamydia trachomatis)
Infectious form of Chlamydia species is elementary in the body.
True
NAD is needed for culturing Chlamydia species.
False
As a result of Stamp-staining chlamydia is green.
False
Chlamydia psittaci can cause conjunctivitis in birds.
True
Rickettsia species can propagate in lymphoid and endothelial cells only.
False, (Rickettsia, Coxiella: propagate in all cell types) and (Ehrlichia, Neorickettsia: lymphoid cells, endothelial cells)
Q-fever is caused by Coxiella burnetti.
True
Mycoplasmas have a pleomorphic shape.
True, pleomorphic pear shaped
Mycoplasmas are obligate anaerobic bacteria.
False, (facultative anaerobic)
Contagious bovine pleuropneumonia is caused by Mycoplasma bovis.
False, (by M. mycoides ssp. mycoides SC
Mycoplasma gallisepticum can cause chronic respiratory disease in birds.
True
Fuchsin is used as counter staining in the Stamp-staining.
False, (3% diluted malachite-green)
The resistance of the reticulated body is higher than the resistance of the elementary body.
False
Trachoma is caused by Chlamydia psittaci in humans.
False, (by chlamydia trachomatis)
All members of the family Rickettsia are obligate cellular parasites.
True
The causative agent of Q-fever needs arthropod vectors, because it cannot survive in the environment for a long time.
False, no vectors are needed
- Cat scratch disease is caused by Rickettsia rickettsii.
False, (by B. henselae)
- Mycoplasmas have typical colony forms, like fried eggs.
True
- Contagious bovine pleuropneumonia is caused by M. mycoides ssp. Mycoides LC.
False, (M. mycoides ssp. mycoides SC)
- Enzootic pneumonia of swine is caused by Mycoplasma hyopneumoniae.
True
- Mycoplasmas are the smallest free-living microorganisms.
True
- Chlamydia pneumoniae has a cell wall.
True
- Chlamydia can be found in the infected host body only.
True
- Chlamydia psittaci causes ornithosis.
True
- Mycoplasma haemofelis can cause cat haemobartonellosis.
True
- Heartwater disease of ruminants is caused by Ehrlichia ruminantium.
True
- Cat scratch disease is caused by Anaplasma sp.
False, (by B. henselae)
- Mycoplasma sp. can cause turbidity in liquid medium.
False, (no turbidity)
Mycoplasma bovis form fried egg shaped colonies on solid media.
True
Contagious bovine pleuropneumonia is caused by M. mycoides spp. mycoides SC.
True
Sabouraud agar is used for culturing fungi.
TRUE
Fungi are able to do photosynthesis.
False, (no photosynthesis)
Mycotoxicosis is caused by fungal toxins.
True
Malassezia pachydermatis has characteristics morphology, bottle-shape cells.
TRUE
Cryptococcus neoformans form mucoid colonies on Sabouraud agar.
True
Dermatophytes cause superficial mycosis.
False
F2 toxin can cause reduced fertility in cattle.
True
Equine epizootic lymphangitis is caused by Histoplasma farciminosum.
True
Dimorphic fungi have yeast form in the host body.
True
Stachybotrys Toxins can cause immunosuppression in horses.
True
- The pH of Sabouraud agar is higher than 6.5.
False, (pH of 5.5)
- Yeasts can be cultured at 37 degrees.
True
Resistance of asexual spores is higher than sexual spores.
False, (sexual spores has higher resistance)
- Malassezia pachydermatis can cause external otitis in dogs.
True
- Microsporum sp. is a member of Dermatophytes.
True
- Candida albicans can be found on mucous membranes of infected animals only.
False, (can be found in the intestinal tracts and in the oral cavities of healthy individuals also)
- Cryptococcus neoformans cannot form colonies on artificial mediums.
True
- Trichophyton verrucosum causes ringworm in cattle.
True
- Aflatoxin is produced by Aspergillus fumigatus.
False, (by A. flavus)
- Penicillin is produced by Penicillium rubrum.
False, (P. notatum, P. chrysogenum)
- Fungi have five phyla.
True
- Funguses are based on the 18S and the 26S ribosomal RNA gene.
True
- Glomeromycota phyla of fungi symbioses with plant roots.
True
- Dermatophytes are obligate anaerobic.
False, (obligate aerobic)
- Ochratoxin is produced by A. ochraceus.
True
- Fumonisins can cause pulmonary oedema in horses.
False, (in pigs)
False, (obligate aerobic)
- Ochratoxin is produced by A. ochraceus.
True
- Fumonisins can cause pulmonary oedema in horses.
False, (in pigs)
ochraceus causes kidney degeneration in cattle.
False, (in poultry and pigs)
- Patulin causes inhibition of ruminal flora.
True
- Fusarium has pigment production.
True
- T2 toxin can cause immunosuppression in cattle.
True, (also poultry and pigs)
- F. moniliforme can cause leukoencephalomalacia in horses.
True
- Stachybotrys atra forms black colonies.
TRUE
- Dimorphic fungi have mould form on media only.
False, (mould and environment)
- B. dermatitis can cause blastomycosis (granulomatosis).
True
- S. schenckii causes subcutaneous granulomatosis in humans.
True, (also dog, cat and horse)
- Coccidioides immitis causes systemic mycosis in humans.
True, (also dog, horse and cat)
- Batrachochytrium dendrobatidis can cause chytridiomycosis of amphibians.
True
- Dermatophytes can be cultured at 37 degrees.
False, (25 degrees)
- Yeasts incubation condition time is 1-4 days.
True
- Dermatophytes incubation condition time is 2-4 weeks.
True
