3rd test - Staphillococcus, Streptococcus Flashcards
Screptococci can be observed in a smear as Gram positive coccoid bacteria forming brunches of grape
True or false
False
Spreptoococci are obligathe pathogenic bacteria
True or false
True
During the Gram staining we use the 96% alcohol to extract safranin or fuchsin from the Gram + bacteria
True or false
false
We can culture Staphylococci selectively by using 10 % NaCl, mannit and phenol-red containing agar.
True or false
True
Staphylococcus intermedius belongs to the group of coagulase negative Staphylococci.
True or false
false
Staphylococcus pseudintermedius causes otitis externa or dermatitis in dog and cat.
True or false
True
A Streptococcus porcinus causes the exudative epidermitis of the pigs.
True or false
False
Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis are important causative agents of mastitis for ruminants.
True or false
False
The Enterococci have a higher pH optimum, compared to the other bacteria.
True or false
True
Lactobacilli, thanks to their active fermentation, can produce lactate in food-this featxxx xxx used in the food industry
True or false
True
Staphylococci can be observed in a smear as Gram positive coccoid bacteria in chains
True or false
True
During the Gram staining we can extract crystal violet and Lugol solutions from Gram + bacteria
True or false
false
MRSA means meticillin resistant Streptococcus agalactioe
True or false
false
We can differentiate between Staphylococci and Streptococci by their pigment production
True or false
True
Staphylococcus aureus, Streptococcus suis and Streptococcus bovis can cause septicaemia in severe cases
True or false
True
The horse strangles is caused by Streptococcus dysgalactiae subsp. equisimilis
True or false
false
The scarlet fever is caused by toxin producing strains of Streptococcus pyogenes
True or false
true
Staphylococci are more fastidious bacteria compared to the Streptococci
True or false
false
The Lactobacilli have a higher pH optimum, compared to the other bacteria
True or false
false
Lactococci and Lactobacilli can be given as probiotics in case of dysbacteriosis
True or false
True
Staphylococcus aureus is a Gram negative coccus
True or false
False
Erysipelothrix rhusiopathiae is the causative agent of dog ulcerative stomatitis.
True or false
False?
Staphylococcus aureus is a haemolytic, pyogen bacteria.
True or false
True
Listeria monocytogenes can propagate themselves (grow) on 4°C
True or false
True
Erysipelothrix rhusiopathiae can porduce capsule around the cell which give resistance against phagocytosis.
True or false
False
Streptococci and Erysipelothrix infection can produce endocarditis in non treated cases.
True or false
True?
The name of Staphylococcus aureus come from that it was first detected on the gold miner skin surface.
True or false
False?
Gram negative strains have a blue color after Gram staining.
True or false
False
Smears were fixated with heat over the flame of bunsen-burner.
True or false
True
Staphylococci can survive in dried exsudatum for long time (e.g.: several months)
True or false
True
Listeria monocytogenes is a Gram positive coccus
True or false
False
Streptococci spp. are apathogen, saprophyte bacteria and belong to the normal skin flora.
True or false
False?
The main coagulase positive species are: Staphylococcus aureus, Staphylococcus intermedius, Stpahylococcus aureus subsp. anaeroblus
True or false
True
Listeria monocytogenes caused main diseases forms are: septicaemia. abortion and encephalitis.
True or false
True
Erysipelothrix rhusiopathiae cannot infect birds or other mammals than swine.
True or false
false
Staphylococcus aureus can produce antibiotics which rarely have resistance among bacteria
True or false
True?
During the Gram-staining method we wash the slide with raw spirit in order to destain the Gram positive bacteria.
True or false
False
Bacteria smears are dried in the refrigerator.
True or false
False
Gram positive strains have a blue color after Gram staining.
True or false
True
Steptococci spp. Take part in formation of the normal skin and mucus microflora
True or false
True
Staphylococcus aureus is a Gram negative coccus
True or false
False
Erysipelothrix rhusiopathiae is the causative agent of dog ulcerative stomatitis.
True or false
False
Staphylococcus aureus is a haemolytic, pyogen bacteria.
True or false
True
Listeria monocytogenes can propagate themselves (grow) on 4 °C.
True or false
True
Erysipelothrix rhusiopathiae can porduce capsule around the cell which give resistance against phagocytosis.
True or false
False
Streptococci and Erysipelothrix infection can produce endocarditis in non treated cases.
True or false
True
The name of Staphylococcus aureus come from that it was first detected, on the gold miner skin surface
True or false
False
Gram negative strains have a blue color after Gram staining.
True or false
False
Smears were fixated with heat over the flame of bunsen-burner.
True or false
True
Staphylococci can survive in dried exsudatum for long time (e.g.: several months)
True or false
True
Staphylococcus
Habitat
occurs world wide
- Commensals on skin, mucous membranes
(upper respiratory tract, lower urogenital)
- Food
- Plant
- Soil
- Water
Staphylococcus
Morphology
- Coccus (1 micrometer in diameter)
- Irregular cluster (buch of grapes)
- Non-motile (NO FLAGELLA)
- Do NOT form SPORES
- NO CAPSULE
Staphylococcus
Culture
- Not fastidious, simple
(nutrient agar, air, 37 degrees, 1 day) - FACULATIVE ANAEROBE (exc S.a.ssp.anaerobius)
- Colony morphology: White, opaque, 1-3 micrometer in diam
- Pigment:
(White, bovine and human-STRAINS - Golden yellow) - S.a.ssp ANAEROBIUS = no pigment production, HAEMOLYSINS (alpha, beta, gamma, delta), Animal trains of S. aureus and S. Pseudointermedius usually produce alpha and beta HAEMOLYSINS
- Selective culture of staphillococci
10% NaCl - Mannitol - Phenolred agar
Na-tellurite - Glycine - Pyruvic Acid - Eggyolk
—-> Black colonies
Blood agar with nalidixic acid and colistrin
Staphylococcus
Biochemistry/ Antigens
Catalase + (exc. S.a.ssp ANAEROBIUS)
Oxidase -
Fermentative
Ag: Complex
Complete hemosysis caused by alpha HAEMOLYSINS
Incompleete hemolysis caused by beta HAEMOLYSINS
(ring figure) - Sheep or ox blood agar
Staphylococcus
Coagulase positive bacterias
S.aureus ssp. Aureus
S. Aureus ssp ANAEROBIUS
S.Pseudointermedius
Staphylococcus
Coagulase negative bacterias
S.Hyicus
S.Epidermidis
S. haemolyticu
S. Gallinarium
S. Equorum
S. Felis
Staphylococcus
Coagulase positive bacterias
S. aureus ssp. AUREUS (biotype A-E)
Local suppuration, abcesses, Infections following surgery:
Many species
- Arthritis (poultry)
- Mastitis (cattle, sheep, goat, pig, horse)
- Metritis (pig)
- Dermatitis (sheep, goat, rabbit, poultry)
- Udder impetigo (cattle, pig)
- Septicaemia (tick pyaemia of 2-5 weeks lamb, rabbit, turkey)
- Botryomycosis in horses (spermatic chord) after castration
Staphylococcus
Coagulase positive bacterias
S. Aureus ssp ANAEROBIUS
Morel disease of sheep (caseous lymphadenitis)
Staphylococcus
Coagulase positive bacterias
S.Pseudointermedius
Dog, Cat:
- Pyoderma,
- Pyometra,
- Otis externa,
- mastitis,
- endometritis,
- cystitis,
- osteomyelitis,
- wound infections
Staphylococcus
Coagulase positive bacterias
S.Hyicus
Exudative epidermis (greasy pig disease): under 3months of age.
Staphylococcus
Coagulase positive bacterias
S.Epidermidis
S. haemolyticu
S. Gallinarium
S. Equorum
S. Felis
Dermatitis
Local suppuration
Staphylococcus
Virulence factor - Pathogenic effect
Coagulase
- Convert fibrinogen to fibrin
- Fibrin deposition may shield staphylococci from phagocytic cells.
Staphylococcus
Virulence factors
- Coagulase
- Lipases
Esterases
Elastases
Staphylo-kinase
deoxyribonuclease
Hyalurenidase
Phospoholipase - Surface proteins (Protein A)
- Leukocidin
- Alpha toxin
- Beta toxin
- Exfoliative toxin
- Enterotoxin
- Toxic shock syndrome toxins
Staphylococcus
Virulence factor - Pathogenic effect
2.
Lipases
Esterases
Elastases
Staphylo-kinase
deoxyribonuclease
Hyalurenidase
Phospoholipase
Extra cellular enzymes which promote tissue invation
Staphylococcus
Virulence factor - Pathogenic effect
3. Surface proteins (Protein A)
Bind to Fc portion of IgG and inhibits Phagocytosis
Staphylococcus
Virulence factor - Pathogenic effect
4. Leukocidin
Cytolytic destruction of Phagocytes
Staphylococcus
Virulence factor - Pathogenic effect
5. Alpha toxin (haemolysin)
Spasm of smooth muscles
necrotizing, lethal
Major toxin in gangrenous mastitis
Staphylococcus
Virulence factor - Pathogenic effect
6. Beta toxin
Damages cell membranes
Staphylococcus
Virulence factor - Pathogenic effect
7. Exfoliative toxin
Responsible for skin lesions in humans
Staphylococcus
Virulence factor - Pathogenic effect
8. Enterotoxin
Heat stable toxins assosiated with staphylococcal food poisoning in humans
Staphylococcus
Virulence factor - Pathogenic effect
9. Toxic shock syndrome toxins
Induce tissue damage in humans
Micrococcus
Habitat
Environment
mucous membranes
food
Skin
air
Micrococcus
Morphology
Coccus
Pigment - yellow, pink, orange
Micrococcus
Biochemistry / Antigens
Catalase +
Oxidase -
OF: O/-
OBLIGATE AEROBIC
Micrococcus
Patogenicity
SAGOPHYTE - not pathogenic
Enterococcus
Habitat
Intestinal tract of animals and man
Enterococcus
Morphology
Some isolates are motile
Enterococcus
Culture
Tolerate bile salts
Grow on MACCOGNEY agar (red pin-point colonies)
10-15degrees
Ph= 9,6
Lancefield D
alpha haemolysis
Enterococcus Biochemistry/antiigens
Previously in group D sthreptococci since they possesses the group D cell wall antigens
Enterococcus
Pathogenicity
Mainly SAPROPHYTES
E.facealis
E. faeciu
E.avium
E.Gallinarium
Sometimes cause endocarditis and abcesses
LACTOCOCUS
Culture
Lancefield N
ANAEROBIC COCCI
PATHOGENICITY
Peptococcus: Metitis, Arthritis, Pneumonia
Peptosthreptococcus
- Peptoniphillus indolicus (bovine summer mastitis)
A. Pyogenies
S. Dysgalactica
Ruminococcus (saphrophyte)
Sarcina(saphrophyte) in rumen, og other non-ruminant in large intestine for monogastric animal grroup
LACTOBACILI
Habitat
Mucous membrane
Gut
food
feed
plant
LACTOBACILI
Morphology
Long, thin, sometimes curved rods
LACTOBACILI
Staining
Gram positive
LACTOBACILI
Culture
Microphillic
Optimal temp at: 30-40 degrees of celcius
LACTOBACILI
Biochemical/ Antigens
Active fermentation
lactate
Ph= 5,0
Silage
LACTOBACILI
Pathogenicity
Saphrophytes
- Delbrucki ssp bulgaricus
- L. Delbrucki ssp lactis
3, L. Acidohillus
4, L, salvarius - L.plantarum
STREPTOCOCCUS
Habitat
- Distributed world wide
- Commensals on skin
- Mucous membranes
(upper resp, lower genital, digestive tract) - Food
- Milk
STREPTOCOCCUS
Morphology and resistance
- Coccus (1micrometer in diameter)
- CHAIN FORMATION
- Capsule (polysaccharide) -
(S.Pyogenes, S.Pneumoniae, S.Equi) - Cell wall M proteins
(S. Pyogenes, S. Equi, S.porcinus) - Anti phagocytic effect
- NO FLAGELLA (non-motile)
- Fimbria (S.pyogenes, S.salivarius, S.Sanguinis)
- NO SPORES
Resistance: Good
Survive for weeks in dark, wet environment
Susceptible to desiccation
STREPTOCOCCUS
Staining
Gram-POSITIVE
STREPTOCOCCUS
Culture
More fastidious
- Require enriched media (blood or serum)
- Facultative anaerobe
- *- CO2 enhance the growth**
- *- Incubation aerobically**, 37 degrees, 24-48 hours
Small translucent colonies
- *-Usually hemolytic**
- *Alpha**: Partial or incompleete - Greenish or hazy zones around the colonies
- *Beta**: Compleete - Clear zones around the colonies
- *Gamma**: No observable haemolysis
No growth on MacCONKEY agar
Selective culture: EDWARDS-AGAR
(Chrystal violet, thallium-SO4, Esculin, blood)
STREPTOCOCCUS
Biochemistry/Antigens
Catalase -
Oxidase -
Fermentative
Glycosides: Esculin, Salicin
Extracellular enzymes and toxins:
Straptolysins (Haemolysins)
Fibrinolysin
Hyaluronidase
DNase
NADase
Streptokinase
Protease
Ag:
Complex
Lancefield- grouping: Cellwall polysaccharide (A-W)
Other specific antigens:
- Capsule antigens:
(S.Pyogenes, S.suis, S.Pneumoniae, S.equi)
Grouping:
Haemolysis
Biochemical characteristics
Antigens
STREPTOCOCCUS
Types
S. Pyogenes
S. Agalactiae, S.dysgalactiae, S.uberis
S.Dysgalactiae ssp equisimilis
S.Equi
S.Equi ssp. equi
S. Equi ssp. zooepidemicus
S.suis septicaemia
S.porcinus lymphadenitis
S. Canis
S. Salivarius, S.Sanguinis, S.Mutans
S.Pneumoniae
STREPTOCOCCUS
S. Pyogenes
Human:
1. Scarlet fever
2. Rheumatic fever
- glomerulonephritis,
- Pharyngitis
- Tonsilitis
3. Human erypelas, Impetigo
STREPTOCOCCUS
S. Agalactiae, S.dysgalactiae, S.uberis
Mastitis
STREPTOCOCCUS
S.Dysgalactiae ssp equisimilis
Lymphadenitis, ABORTION in HORSES
Because of abort, no milk production = dysgalactic
STREPTOCOCCUS
S.Equi
S.Equi ssp. equi
S. Equi ssp. zooepidemicus
- S. Equi ssp. equi = STRANGLES in HORSES
- S.Equi ssp. zooepidermicus
= Suppuration, septicemia in human, mastitis
STREPTOCOCCUS
S.suis septicemia
S.porcinus lymphadenitis
S.suis septicemia - ARTHRITIS, MENINGITIS
S. Porcinus lymphadenitis = Abcesses
STREPTOCOCCUS
S. Canis
Metritis
Septicaemia
STREPTOCOCCUS
S. Salivarius, S.Sanguinis, S.Mutans
ORAL STREPTOCOCCI
STREPTOCOCCUS
S.Pneumoniae
Pneumonia (human, guinea-pig and rat)
Meningitis
Staphylococcus
Staining arangement
Spore
Motillity
Growth in air
Growth in anaerobe environment
Catalase
Oxidase
OF test
Staining arrangement
= Gr+, irregular arrangemet, bunch of grapes
Spore = NO -
Motillity= NO-
Growth in air= YES+
Growth in anaerobe environment= YES +
Catalase = YES +
Oxidase = NO-
OF test= F
Streptococcus
Staining arrangement
Spore
Motillity
Growth in air
Growth in anaerobe environment
Catalase
Oxidase
OF test
Staining arrangement
= Gr+, in CHAINS, (from broth culture, pus, milk)
Spore = NO -
Motillity= NO-
Growth in air= YES+
Growth in anaerobe environment= YES +
Catalase = NO +
Oxidase = NO-
OF test= F
MICROCOCCUS
Staining arrangement
Spore
Motillity
Growth in air
Growth in anaerobe environment
Catalase
Oxidase
OF test
Staining arrangement
= Gr+, alone, in pairs, irregular clusters, packages of 4/6
Spore = NO -
Motillity= NO-
Growth in air= YES+
Growth in anaerobe environment= NO -
Catalase = YES +
Oxidase = YES+
OF test= O/-
ANAEROBE COCCUS
Staining arrangement
Spore
Motillity
Growth in air
Growth in anaerobe environment
Catalase
Oxidase
OF test
Staining arrangement
= Gr+, alone, in chains, Irregular clusters, packages of 4
Spore = NO -
Motillity= NO-
Growth in air= NO-
Growth in anaerobe environment= YES +
Catalase = NO -
Oxidase = NO-
OF test= F/-
RHODOCOCCUS
Staining arrangement
Spore
Motility
Growth in air
Growth in anaerobe environment
Catalase
Oxidase
OF test
Staining arrangement
= Gr+, Cocci or rods
Spore = NO -
Motillity= NO-
Growth in air= YES+
Growth in anaerobe environment= NO -
Catalase = YES +
Oxidase = YES+-
OF test= -
Staining arangement
STAPHYLOCOCCUS
STREPTOCOCCUS
MICROCOCCUS
ANAEROBE COCCUS
RHODOCOCCUS
All GR +