Bacteriology Flashcards
Last step in bacterial CW synthesis
Cross-linking
Fixes primary stain
Mordant
Mordant in Gram staining
Gram iodine
Decolorizer in Gram stain
Acetone alcohol
Identification of Chlamydia in low-resource area
Giemsa
Special stain: Legionella
Dierle (silver stain)
G- cocci
Neisseria
Branhamella/ Moraxella
Veillonella
Microorg: Wright stain
Borrelia
Special stains: P. carinii
Silver stain
Gomori methenamine stain
Microorg: Calcoflour white
Fungi
Cold method of staining acid fast microorgs
Kinyoun
Bacterial organelle responsible for encoding genes that regulate non essential life functions
Plasmid
Bacterial organelle responsible for encoding bacterial resistance
Plasmid
Pseudocapsule
P. aeruginosa
MCC of overwhelming postsplenectomy infection
S. pneumoniae
Dse conditions that makes host most susceptible to encapsulated microbe infections
Postsplenectomy
Sickle cell anemia
Biofilm on catheter-related infections
S. epidermidis
Biofilm in dental plaque/ dental caries
S. mutans
Metachromatic staining
Corynebacterium, due to Babes Ernst granules
Kidney-shaped diplococci
Neisseria
Lancet-shaped diplococci
S. pneumoniae
Bipolar staining
Yersinia
Gull-wing
Campylobacter
Post C. jejuni conditions
GBS
Reactive arthritis/Reiter syndrome
Inactivating EF2 (elongation factor 2)
1) A-B toxin of C. diphtheriae
2) Exotoxin A of P. aeruginosa
Exotoxin vs endotoxin: Meningococcemia
Endotoxin
A-B toxin causing ADP ribosylation of G protein
V. cholerae
Pea soup diarrhea
Salmonella typhi (???V. cholera)
HUS: Toxins
Shiga toxin
Shiga-like toxin
HUS: Microorg
Shigella
EHEC
Dse: A-B toxin that inhibits G-alpha
Whooping cough
Use inorganic cmpds
Autotrophs
Use organic compounds as energy source
Heterotrophs
Best grow at 0-20C
Psychrophile
Best grow at 20-40C
Mesophile
Best grow at 40-80C
Thermophile
Bacterial growth curve, phase: Metabolic activity
Lag
Bacterial growth curve, phase: Rapid cell division
Log
Bacterial growth curve, phase: Nutrient depletion
Stationary
Destroys all life forms
Sterilization
Destroys disease-causing orgs; not all lifeforms
Disinfection
Postulate: Microorganism must be isolated and grown in pure culture; must cause dse in healthy indiv; must be reisolated and identified
Koch’s postulate
Standard for new chemical antimicrobials
Phenol
Autoclave conditions to kill spores
121C at 15 psi for 15-20 mins
Rapid freezeing or freeze-drying
Lyophilization
Used to preserve cultures
Lyophilization
Sporicidal chemical agent
Halogen
Most effective skin antiseptic
Iodine
Ethylene oxide
Used for cold sterilization
Ethylene oxide
Neisseria culture medium: From sterile site
CAP
Neisseria culture medium: From unsterile site
Thayer Martin
Satellite phenomenon on culture
H. influenzae
Charcoal in culture medium
Bordetella
Legionella
Mercury drop colonies on charcoal medium
Bordetella pertussis
Eaton agent
Mycoplasma
Egg yolk agar
C. perfringens
BSK medium
Borrelia
Staib’s medium
C. neoformans (Bird seed agar)
Bird see agar
C. neoformans (Staib’s medium)
Bile esculin agar
Group D enterococcus
Castaneda medium
Brucella
Skirrow’s agar
C. jejuni
H. pylori
TCBS
V. cholerae
EMJH
L. interrogans
AIDS, MC bacterial infection
M. avium intracellulare
AIDS, MC fungal infection
P. carinii
AIDS, MC protozoal infection
T. gondii
Cryptosporidium
AIDS, MC viral infection
CMV
AIDS, benign viral infection
Molluscum contagiosum (poxvirus)
Lumpy jaw
A. israelii
Golden yellow colonies of S. aureus is due to
Staphyloxanthin
Normal flora of skin
S. epidermidis
S. aureus
Normal flora of nasopharynx
S. aureus
Normal flora of mouth and throat
S. viridans
Actinomyces
Normal flora of colon
B. fragilis > E. coli; enterococci
Normal flora of vagina
L. acidophilus
GBS
E. coli
Normal flora in gallbladder
Salmonella typhi
Pneumonia in alcoholics
S. pneumoniae
Pneumonia in neonates
GBS
E. coli
Listeria
Pneumonia in 1mo-3mos
RSV
Pneumonia in young adults
Mycoplasma
Lobar pneumonia
S. pneumoniae
Pneumonia in neutropenic patients
P. aeruginosa
Pneumonia in AIDS
P. jiroveci/carinii
Pneumonia in barracks
Mycoplasma
Atypical pneumonia
Mycoplasma
Pneumonia in cystic fibrosis
Pseudomonas
Pneumonia in diabetics
Pseudomonas
Pneumonia in CGD
Pseudomonas
Pneumonia in poorly nourished, unvaccinated child
Measles
Giant cell pneumonia
Measles
Mech vent assoc pneumonia
Pseudomonas
Hospital-acquired pneumonia
Pseudomonas
All capsular bacteria have polysaccharide capsule except
B. anthracis
All viruses are haploid except
Retroviridae
Raw eggs
Salmonella
Reheated meat
C. perfringens
Mayonnaise
S. aureus
Hamburger
EHEC
Custard
S. aureus
Apple juice
EHEC
Alfalfa sprouts and spinach
EHEC
Bilateral eyelid swelling/periorbital edema
Trichinella spiralis
Sty
S. aureus
Sticky eye
Propionibacterium
MCC of blindness worldwide
Chlamydia
Chorioretinitis
CMV
Toxoplasma
Congenital retinopathy
Treponema
Pebbly conjunctivitis
Chlamydia
Corneal scarring
Chlamydia
Congenital cataract
Rubella
Meningitis in neonates
GBS
E. coli
Listeria
Meningitis in 2-6 mos, unimmunized
H. influenzae
Meningitis in adults
S. pneumoniae
Meningitis outbreaks in crowded conditions
N. meningitidis
Meningitis in adolescents and young adults
N. meningitidis
Meningitis in barracks and college dorms
N. meningitidis
Meningitis in AIDS
Cryptococcus
Toxin responsible for pneumatocele formation by S. aureus
Panton valentin leukocidin
Toxin: SSSS
Exfoliatin
Most severe form of SSSS
Ritter disease
Acute mastitis
S. aureus
Infective endocarditis in IV drug users
S. aureus
MC valve involved in infective endocarditis in IV drug users
Tricuspid
MCC of osteomyelitis in the general population
S. aureus
Meningitis in 2-6 mos, immunized
S. pneumoniae
Peritonitis in renal failure patients
S. epidermidis
MCC of subacute endocarditis
S. viridans
MCC of acute endocarditis
S. aureus
MCC of endocarditis in patients with prosthetic heart valves
S. epidermidis
MCC of osteomyelitis in sexually active individuals
N. gonorrheae
MCC of osteomyelitis in IV drug abusers
P. aeruginosa
S. aureus virulence factor: Food poisoning
Enterotoxin A
S. aureus virulence factor: Infective endocarditis
Fibrin-platelet mesh
S. aureus virulence factor: Impetigo
Coagulase
Staphylokinase
Impetigo
S. aureus
GABHS
Septic arthritis in prosthetic joints
S. epidermidis
Strep antigen: Rheumatic fever
M protein
Strep strain responsible for PSGN
M12
S. pyogenes toxin: Hemolysis
Streptolysin S
MCC of impetigo
S. aureus
MCC of bullous impetigo (80%)
S. aureus