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1. microbiology > Bacterial Culture > Flashcards

Bacterial Culture Flashcards

1
Q

What is a culture medium?

A

A water solution of various nutrients that encourage the growth or organisms

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2
Q

What does a culture medium generally contain?

3

A

Energy

Carbon, nitrogen and other essential nutrients

Growth factors (may be added to encourage the growth of a particular fastidious organism)

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3
Q

What two forms can culture medias come in?

A

Liquids or gels

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4
Q

What does the success of culture depend on?

A

Selection of appropriate culture media

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5
Q

What are the five different types of medias?

A

General purpose media

Enriched media

Selective media

Differential media

Selective and differential media

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6
Q

Give an example of a general purpose media.

A

Horse blood agar

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7
Q

How is horse blood agar a general purpose media?

A

It supports the growth of most gram-positive and gram-negative bacteria

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8
Q

What is horse blood agar made from?

A

Proteins sources

Sodium chloride

5% blood

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9
Q

What is enriched media?

A

Media fortified with yeast extracts or protein infusions, useful in growing fastidious organisms

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10
Q

What are enriched medias fortified with?

A

Yeast extracts

OR

Protein infusions

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11
Q

Give an example of an enriched media.

A

Chocolate agar

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12
Q

What is present in chocolate agar?

A

Red blood cells that have been lysed

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13
Q

Name two fastidious organisms that require chocolate agar.

A

Neisseria meningitis

Haemophilus influenzae

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14
Q

What are selective medias?

A

Selective medias encourage the growth of one organism whilst suppressing the growth of another

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15
Q

Give an example of a selective media.

A

MacConkey agar

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16
Q

What does MacConkey agar contain that act as selective agents?

A

Bile salts and crystal violet

17
Q

What is MacConkey selective for?

A

Gram-negative rods

18
Q

What are differential medias?

A

They differentiate target organisms from a mixed culture

19
Q

Give an example of a differential media.

A

MacConkey agar

20
Q

What are the differential agents in MacConkey agar?

A

Peptone

Lactose

Neutral red dye

21
Q

Give an example of a selective differential media.

A

Mannitol salt agar

22
Q

What is the selective agent in mannitol salt agar?

A

High salt content

23
Q

What is the differential agent in Mannitol salt agar?

A

Mannitol and the indicator phenol red

24
Q

What is needed for anaerobic growth to be achieved?

A

An anaerobic jar or cabinet with all the oxygen removed

1. microbiology (12 decks)

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