BACTERIAL CULTIVATION Flashcards
Refers to increase in the number of bacteria
Bacterial growth
Nutritional requirements of a bacteria
Carbon, nitrogen, energy (CNE)
Needed for the synthesis of cellular components
Carbon
Dry weight of Carbon
50%
Carbon dioxide from the air
Autotrophs
Organic compound in culture media
Heterotrophs
What form of energy does bacteria needed?
Adenosine triphosphate (ATP)
Light source
Phototrophs
Chemical energy
Chemotrophs
Needed for synthesis of proteins
Nitrogen
Nitrogen compounds in culture media
Peptone, yeast, beef extract
Inorganic molecule
Lithotrophs
Organic molecule
Organotrophs
Dry weight of Nitrogen
14%
Dry weight of smaller molecules
4%
Phosphate
Nucleic acid
Phospholipids
Cell membrane
Sulfur
Protein synthesis
Serves as a co factors
Mineral elements
Bacteria that can survive in a high concentration of salt
Halophilic or halophiles
Bacteria that are very difficult to grow
Fastidious
X FACTOR
Hemin or hematin
V FACTOR
Nicotanamide adenine dinucleotide
A specie that requires additional growth requirements
Haemophilus spp.