BACTERIAL CELL COMPONENTS Flashcards

1
Q

Other term for cell wall

A

Peptidoglycan or murein layer

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2
Q

Give the 2 other components of cell wall

A

Phospholipid bilayer and proteins

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3
Q

Most important purpose of the cell wall

A

Location for Electron transport chain (energy generation)

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4
Q

It gives rigidity to the cell wall

A

Teichoic acid

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5
Q

It regulates muramidase - a cell wall enzyme

A

Lipoteichoic acid

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6
Q

Streptococcus pneumoniae

A

Gram positive organism

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7
Q

Staphylococcus aureus

A

Gram positive organism

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8
Q

It has a thick murein layer

A

Gram positive cell wall

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9
Q

It has a thin murein layer

A

Gram negative cell wall

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10
Q

It has a perplasmic space

A

Gram negative cell wall

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11
Q

Crystal violet rinsed vigorously before gram’s iodine complex

A

False gram negative

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12
Q

Prolonged decolorization

A

False gram negative

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13
Q

Insufficient decolorization

A

False gram positive

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14
Q

Insufficient primary stain application

A

False gram positive

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15
Q

Antibiotic treated, degenerating old culture

A

Gram ghost (only in gram positive)

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16
Q

A characteristic exhibited by gram positive bacteria

A

Gram variability

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17
Q

Natural gram variability

A

Mobiluncus spp. and Gardnerella vaginallis

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18
Q

Gram negative cocci

A

Neisseria spp., Veillonella spp., Moraxella spp. (NVM)

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19
Q

All bacilli are negative except?

A

MyBELLCRACK

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20
Q

Bacteria that are gram negative because the do not have a CELL WALL

A

Mycoplasma and Ureaplasma

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21
Q

Spiral (gram negative or gram positive?)

A

Gram negative

22
Q

Contains 60% hydrophilic lipids, mainly mycolic acid, that affects the staining

A

Mycobacterium and Nocardia

23
Q

A staining method that is best for bacteria containing long chain of fatty wax (mycolic acid)

A

Acid fast staining

24
Q

It renders the resistance of bacteria to decolorizers

A

Mycolic acid

25
Q

Most common Acid Fast Bacteria

A

Mycobacteria (Mycobacterium tuberculosis)

26
Q

Most crucial step in staining

A

Decolorizing

27
Q

Color of acid fast bacteria

A

Pink or red

28
Q

Color of non-acid fast bacteria

A

Blue

29
Q

Decolorizer of Ziehl-Neelsen method

A

3% Hydrochloric acid (HCl) and 95% ethanol

30
Q

Why do Kinyuon method does not require heating?

A

Because of high concentration of phenol in primary stain

31
Q

Decolorizer of Kinyuon Method

A

3% Sulfuric acid (H2SO4) and 95% ethanol

32
Q

Color of stain in Fluorescent method

A

Bright yellow/orange against dark

33
Q

A phospholipid bilayer with proteins

A

Cytoplasmic/plasma membrane

34
Q

This is where the nucleoid region is attached

A

Mesosomes

35
Q

Responsible for protein synthesis

A

Free ribosomes

36
Q

Storage of excess nutrients

A

Inclusion bodies

37
Q

It contains lipids

A

Much granules

37
Q

Contains polyphosphates

A

Volutin

38
Q

Safety pin appearance

A

Bipolar bodies

39
Q

Storage of excess nutrients

A

Inclusion bodies

40
Q

Special stain for bipolar bodies

A

WAYSON and Methylene blue

41
Q

Resting/hibernating stage of bacteria when exposed to unfavorable environment

A

Endospores/bacterial spores

42
Q

Shorter and thinner than flagella; Mostly found in gram negative bacteria

A

Pili or Pilus

43
Q

A pili that is used for adherence to one another, host, cells, and environment surfaces

A

Common/somatic/Ordinary Pili

44
Q

A pili that is responsible for bacterial conjugation

A

Sex/Fertility/F Pilus

45
Q

This allows the bacteria to move from one place to another

A

Flagella or flagellum

46
Q

Protein found in flagella

A

Flagellin

47
Q

Helpful for serological typing and identifying species (Salmonella spp.)

A

H antigen

48
Q

Uniform and condensed material that is firmly attached to the bacteria

A

Capsule

49
Q

Loose, thick, viscous, unorganized material that is not firmly attached to the bacteria

A

Slime layer