Bacterial biochem

Question 1

What is the difference between substrate level phosphorylation and oxidative phosphorylation?

Answer 1

Oxidative phosphorylation is the synthesis of ATP via the electron transport chain

Substrate level phosphorylatioin is indicative of ATP being produced anywhere that does not involve the use of the electron transport chain

Question 2

What is the key difference between aerobic and anaerobic respiration?

Answer 2

Aerobic = uses oxygen as terminal electron acceptor

Anaerobic = uses anything else as terminal electron acceptor

–>Nitrate and sulfate are examples

Question 3

Where is the ETC located in prokaryotes?

Answer 3

Cytoplasmic membrane

Question 4

in measuring bacterial growth, one can use a closed or open system. What is the difference between the 2?

Answer 4

Closed: nutrient not renewed, waste not removed

Open: Nutrients added and waste products removed

*under these conditions, bacteria populations predictably grow in a particular pattern

Question 5

What are the 5 (4) phases of a bacterial growth curve?

Answer 5

Lag

Exponential (log)

Stationary

Death phase

Question 6

What are the qualities of the lag phase?

Answer 6

shift in envrionment whereby bacteria are adjusting and are unable to replicate just yet

Question 7

What is the exponential or log phase?

Answer 7

Bacteria mutliply rapidly at a constant rate- this is when generation time can be measured

*phase is clinically significant bc many antibiotics affect actively dividing cells*

Question 8

What is the stationary phase?

Answer 8

Occurs when cells have exhausted their nutrient supply

Question 9

What is the death phase?

Answer 9

Viable cell numbers decrease exponentially but not at the rate at which they increased during the log phase