Bacteria Genetics Flashcards
Define genetics
the study of genomes and DNA/RNA, genome replication, gene expression, genetic variation and distribution
Why study bacterial genetics?
- Bacteria are important causes of infection as well as key components of the microbiome
- Bacteria are important industrial organisms
- Bacteria have small, single-copy genomes that are relatively simple and easy to study
- Many exciting tools for manipulating genomes also come from bacteria.
What does the bacteria DNA include?
Chromosome (single copy, circular, essential for life)
▪ Mobile genetic elements (MGE), such as
What are the two types of mobile genetic elements?
● Plasmids (autonomously replicating circular DNA)
● Prophage (viruses integrated into the chromosome)
Describe the genome of MRSA
Chromosome is 2.9 million bp
o Carries integrated prophage, transposons, pathogenicity islands, antimicrobial resistance elements
o Encodes roughly 2800 genes
Putative genes:
encoding proteins with predicted functions (e.g. toxins, virulence factors, metabolic pathways)
What is SNP?
single nucleotide polymorphism- DNA polymerase error
What can errors be?
advantageous, detrimental or neutral
What is mobile genetics elements?
- Horizontal transfer of MGE
- Many MGEs encode virulence, antimicrobial resistance or host-specific genes.
- Acquisition of MGEs can lead to new bacterial variants with enhances virulence or resistance or host range.
How MGEs move between bacteria?
Conjugation- The process that exchanges genetic information between two bacteria through a direct cell-to-cell contact is known as conjugation.
Transduction- The process of transferring bacterial DNA from one cell to another cell by means of bacteria-infecting viruses is known as transduction.
Transformation- The process of taking the free fragments of DNA by bacteria from the surrounding medium is known as transformation.
Recall the parts the structure of plasmids
Selective marker
promotor
5’primer site
inserted gene
restriction site
3’ primer site
origin of replication
antibiotic resistance gene
Where are antimicrobial resistance genes normally found in the bacteria
plasmid
Which type of exchange is most used by plasmids?
conjugation
How is transformation used in the liver?
artificially move purified plasmids into bacteria
How do bacteriophage work?
The phage genome pops out of the chromosome and circularises
▪ The circular phage can pop back in to the chromosome and it can go on to replicate and make many copes
Lysogenic phage
integrated into the bacterial chromosome. Phage in this state is called prophage
Viral DNA being the part of bacterial chromosome passes to each daughter cell in all succeeding generations.
Sometimes, nevertheless, the viral DNA gets detached from the host’s chromosome and the lytic cycle starts.