Bacteria & Cloning

Question 1

Synthetic lethality is…

Answer 1

double mutants are inviable, but both single mutations on their own are viable

Question 2

Transgenic organisms are…

Answer 2

organisms containing DNA sequences that have been created in vitro and introduced into the genome (transgenic sequences)

Question 3

Define transposon.

Answer 3

A gene that can move within the genome.

Question 4

Transposon tagging systems can…

Answer 4

-can be used in any organisms (even if not naturally having transposons)
-The transposase gene is removed from the transposon used in tagging to prevent the transposon from moving to other locations.
-can be used as a probe
-The inverted repeats are removed from the transposon used in tagging to prevent the transposon from moving to other locations.

Question 5

Reporter genes.

Answer 5

transgenes that produce visual output of gene expression patterns/ provides info about gene expression eg. lac- used to study bacteria & animals