Bacteria Flashcards

1
Q

What does Staphylo, Strepto and Diplo refer to

A

Staphylo= clustered
Strepto= Straight chains
Diplo=Clusters of 2 bacterial cells

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2
Q

Difference bw prokaryotic and eukaryotic ribosome

A

Pro- 70s (make of 50s and 30s)
16 RNA makes them unique

Euk- 80s (made of 60s and 40s)

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3
Q

What do bacterial cell walls contain

A

Peptidoglycan

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4
Q

What do Gram positive bacteria have and what makes the cell wall strong

A

Thick peptidoglycan layer w/ NAM and NAG cross links

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5
Q

What do Gram negative bacteria have

A

Double plasma membrane with thin cell wall in between with less peptidoglycans

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6
Q

What color will gram positive and negative bacteria turn

A

positive- purp

neg- pink

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7
Q

What are the 2 toxins released by bacteria

A
  1. Exotoxins

2. Endotoxins- refers to lipo poly saccharide which is a major component of gram neg bac

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8
Q

What is lipopolysaccharide composed of

A

O antigen
Core oligosaccharide
Lipid A

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9
Q

Steps of Gram staining

A
  1. Crystal violet binds thick PG layer
  2. Iodine fixes stain
  3. Alcohol removes weakly bound CV
  4. Sadranin added to decolorized bac
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10
Q

What are all bacteria surrounded by (2)

A
  1. Capsul made of glycocalyx

2. Slime made of biofilm

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11
Q

What is a biofilm and how is it made

A

aggregation of microorganisms in which they stick to eachother and also a surface

-start from a single planktonic cell that attaches to a surface and starts producing slime. Overtime more attach and large slime layer is produced (may protect agains antibiotics)

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12
Q

What are fimbriae and pili

A

fimbriae- bristle appendages that are used to adhere to host cells

Pili- used for bacterial conjugation (sex pilus)

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13
Q

What are secretion systems used for and what type of bacteria are they found on

A

Found in some gram negative bacteria to inject exotoxins into target cells.

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14
Q

What are the genetics of the bacteria stored in the cell

A

Nucleoid region and

Extrachromosomal circles called plasmids (not essential for survival)

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15
Q

What happens in binary fission

A

Bacteria primary reproduce asexualy

no increase in genetic diversity

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16
Q

What type of bacteria produce endospores and what are its characteristics

A

Certain gram positive bacteria

Stripped down dormant form that is highly resistent

17
Q

Faculative anaerobic bac
Microaerophiles
Aerotolerant anaerobes

A

faculative- will use O2 if available but not required
Microaerophiles- require O2 but poisened by too much
Aerotolerant- dont require oxygen but not posioned by O2

18
Q

What type of colonies grow on the bottom of thioglycollate broth test tube

A

Anerobic (hard for o2 to get that deep)

19
Q

Intracellular bacteria vs facultative intracellular

A

Intracellular- entirely dependent on host cells

Facultative- can survive inside or outside

20
Q

what does blood agar test for and what is:
B-hemolysis
A-hemolysis
y- hemolysis

A

Tests for ability to lyse RBCs

B- complete hemolysis
A- Partial hemolysis
y- no hemolysis

21
Q

What are probiotics and prebiotics

A

Pro- live microorganisms intended to provide healath benifits by improving gut flora

Pre- Indigestible fibres to feed bacteria in gut