Bact PNA II (lec 13)

Question 1

Haemophilus influenzae microbe characteristics?

Answer 1

G- coccobacillus
Lipooligosacc (LOS) cell wall
Cap and non-cap
Need RBC factors for growth

Question 2

H. flu Virulence Factor?

Answer 2

Only Non-typeable strains cause dz

Polyrib phosphate CAPSULE:
Φ phago
Fimbriae attachement
Φ IgA

Question 3

H. flu epidemiology?

Answer 3

Opportunistic
Type b (Hib) -> PNA babies

Question 4

H. flu action?

Answer 4

bind ciliated epith cells ->
Secrete LOS ->
↓ cilia, inflamm

Question 5

H. flu diagnostic tests?

Answer 5

Latex Agg = best test

Gram stain
Serology = capsulated
Cx (chocolate agar) = satellites around other bacteria
Catalase = +

Question 6

H. flu tx?

Answer 6

amox

vaccine

Question 7

Legionella pneumophila microbe characteristics?

Answer 7

G- bacillus: thin/pleomorphic
Fimbriae
β-lac prdxn
Polar flagellum
Catalase/Oxidase +

Question 8

L. pneumo action?

Answer 8

Opsonized w/ C3b ->
phagocytosed ->
multiply in phago ->
phago lysed / toxin released

Question 9

L. pneumo epidemiology?

Answer 9

(U) immuno/pulmonary compromised

Moist environ (in H2O)
Replicate in protozoa
Aerosole transmission (NO p-to-p)
(U) serogroup 1

Question 10

L. pneumo causes what 2 dzs?

Answer 10

1) Pontiac Fever

2) Legionnaire’s Dz

Question 11

Pontiac Fever presentation?

Answer 11

flu-like sxs
No PNA signs
self-limit (2-5 days)

Question 12

Legionnaire’s Disease presentation?

Answer 12

Severe, acute Atypical PNA
PNA sxs w/ GI and neuro
High mortality (shock/resp fail)

Question 13

L. pneumo diagnostic tests?

Answer 13

Cx on Buffered Char Yeast

Rapid urine antigen

Question 14

L. pneumo DOC?

Answer 14

levofloxacin

Question 15

Pseudomonas aeruginosa microbe characteristics?

Answer 15

G- motile bacillus
Aerobic
Single flagellum
Blue/green color

*used in bioremediation

Question 16

P. aeruginosa Virulence Factor?

Answer 16

Pyocyanin:
ROS pdxn

Exotoxin A:
ciliastasis
immunosupp

Elastases A/B:
Lyse elastin

Alginate Glycocalyx:
Adhesion
Φ phago
Φ mucociliary escalator

Lipopolysacc Endotoxin

Question 17

P. aerug epidemiology?

Answer 17

Opportunistic
Widespread: plants, water, soil
Skin/feces, food spread
Many kinds of infections

Question 18

P. aerug diagnostic tests?

Answer 18

Tinged sputum
Isolation: water-soluble blue/green, fruity smell

Must run sensitivity (highly resistant)

Question 19

P. aeurg nosocomal PNA DOC?

Answer 19

Cefepime + levoflo

Avoid broad-spectrum (suppresses normal flora)