Bact PNA II (lec 13) Flashcards
Haemophilus influenzae microbe characteristics?
G- coccobacillus
Lipooligosacc (LOS) cell wall
Cap and non-cap
Need RBC factors for growth
H. flu Virulence Factor?
Only Non-typeable strains cause dz
Polyrib phosphate CAPSULE:
Φ phago
Fimbriae attachement
Φ IgA
H. flu epidemiology?
Opportunistic Type b (Hib) -> PNA babies
H. flu action?
bind ciliated epith cells ->
Secrete LOS ->
↓ cilia, inflamm
H. flu diagnostic tests?
Latex Agg = best test
Gram stain
Serology = capsulated
Cx (chocolate agar) = satellites around other bacteria
Catalase = +
H. flu tx?
amox
vaccine
Legionella pneumophila microbe characteristics?
G- bacillus: thin/pleomorphic Fimbriae β-lac prdxn Polar flagellum Catalase/Oxidase +
L. pneumo action?
Opsonized w/ C3b ->
phagocytosed ->
multiply in phago ->
phago lysed / toxin released
L. pneumo epidemiology?
(U) immuno/pulmonary compromised
Moist environ (in H2O)
Replicate in protozoa
Aerosole transmission (NO p-to-p)
(U) serogroup 1
L. pneumo causes what 2 dzs?
1) Pontiac Fever
2) Legionnaire’s Dz
Pontiac Fever presentation?
flu-like sxs
No PNA signs
self-limit (2-5 days)
Legionnaire’s Disease presentation?
Severe, acute Atypical PNA
PNA sxs w/ GI and neuro
High mortality (shock/resp fail)
L. pneumo diagnostic tests?
Cx on Buffered Char Yeast
Rapid urine antigen
L. pneumo DOC?
levofloxacin
Pseudomonas aeruginosa microbe characteristics?
G- motile bacillus
Aerobic
Single flagellum
Blue/green color
*used in bioremediation
P. aeruginosa Virulence Factor?
Pyocyanin:
ROS pdxn
Exotoxin A:
ciliastasis
immunosupp
Elastases A/B:
Lyse elastin
Alginate Glycocalyx:
Adhesion
Φ phago
Φ mucociliary escalator
Lipopolysacc Endotoxin
P. aerug epidemiology?
Opportunistic
Widespread: plants, water, soil
Skin/feces, food spread
Many kinds of infections
P. aerug diagnostic tests?
Tinged sputum
Isolation: water-soluble blue/green, fruity smell
Must run sensitivity (highly resistant)
P. aeurg nosocomal PNA DOC?
Cefepime + levoflo
Avoid broad-spectrum (suppresses normal flora)
