B7 - practicals Flashcards

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1
Q

What do you need to do to make sure your working safely in a lab?

A

Wear lab coat to protect skin and clothing
Wear safety googles
Wear gloves if the chemical is an irritant
Keep anything flammable away from Bunsen burner if used
Do not touch hot equipment directly
Handle glassware carefully so it doesn’t break.

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2
Q

What are the ethical issues when organisms are involved.

A

Animals need to be treated humanely - handled carefully and returned to original habitat

If involving humans they should not be forced to participate against their will or feel pressured

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3
Q

A student wants to test the distribution of an organism in an area. She takes sample of one part of an area. What could she do better.

A

Random sampling to give a more accurate representation of the whole area.
Divide the field into a grid .
Label the grid with numbers.
Use a random number generator to select coordinates
Take your samples with a quadratic at these coordinates.

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4
Q

How to measure length?

A

Small - ruler
large - metre stick or tape measure
The smaller unite have a higher degree of accuracy

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5
Q

How to measure area?

A

Measure length and width
Area of a rectangle = length * width
area of circle - pie r^2
Area of triangle - 1/2 b*h

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6
Q

How to measure mass

A

To weigh a solid,
Start by putting the container you are weighing your substance into on a balance
Set the balance to 0
Then weigh out the correct amount of substance.

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7
Q

How to measure time

A

Use a stopwatch - measures to the nearest 0.1s so it is more accurate.
Same person should do the timing so results are more precise.

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8
Q

How to measure temperature?

A

Use a thermometer to measure the temperature

Read scale on thermometer at eye level

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9
Q

How to measure volume of a liquid

A

Pipette - dropping pipette is used to transfer drops of liquid
Graduate pipette is used to transfer accurate volumes
Always read volume from bottom of meniscus

Measuring cylinder

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10
Q

A student counts the number of bubbles produced by the pondweed. How can she make this practical better and why is this not the best method.

A

It is not the best method because it is less accurate and does not tell you the total volume produced.
A better way is to measure volume of gas using gas syringe.
Or use a upside down measuring cylinder filled with water, the gas will displace the water so you can read the volume off the scale.

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11
Q

A students is investigating amount of gas produced however there is a gap in her experiment. What could this do to her results ?

A

Gas can escape so it will not be accurate

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12
Q

How to measure pH

A

Indicators change colour depending if it is an acid or an alkali.

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13
Q

A student wants to measure pH but doesn’t want the entire solution to turn a different colour. What should she do ?

A

Use indicator paper, the paper touches the solution and it will change colour .
Or use a pH meter to digitally display an accurate value.

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14
Q

How to measure heart rate?

A

Find your pulse on the wrist . Count the number of beats in 15 seconds and multiply by 4 to get the number per minute.

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15
Q

How to use a Bunsen burner safely?

A

Use a heat proof mat
Always put on clear visible flame, safety flame, if not using
Use the top of the blue flame to heat
If heating something over flame, e.g. beaker of water, use a tripod and gauze

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16
Q

A student would like to heat a beaker of water at a constant temperature what should she do?

A

Use a water bath , which can be turned to a specific temperature.
Use a thermometer to check it is the correct temperature

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17
Q

A student would like to take continuous samples, but redoing practicals again and again doesn’t seem practical. What is a good method for this.

A

Data loggers. - decide what you are measuring. Connect the external sensor to the data logger. Decide how often you want the data logger to take readings.
Computer will view the data and to process it draw graphs.
It is more accurate and you can take larger sample size.

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18
Q

One scientist thinks that the results of 105 bubbles at 12.5cm away may be an error.
Describe how she can be more certain that 105 Is the true value

A

Repeat the reading for 12.5cm

If the number of bubbles is close to 105 then she can be more certain it is the true value.

19
Q

How could similar apparatus be used to allow the scientists to investigate the effect of temperature on the rate of photosynthesis.

A

Use a water bath to vary the temperature and count the number of bubbles given off at each temperature

20
Q

A student is carrying out a field investigation to determine the population of woodlice in the schools wildlife garden.
Describe a method the student could use to determine the population size of woodlice.

A

Mark the individuals
Release the individuals
Collect a second sample and count the number of marked individuals
Use equation
Number 1st sample * Number 2nd Sample / Number 2nd marked

21
Q

Woodlice are often found under logs and bark where it is damp.

Suggest why woodlice prefer damp places.

A

Loses less water

Water is required for decomposition by microorganisms of food source.

22
Q

Nina wants to investigate how changing the light intensity affects the rate of water uptake by a leafy shoot. Describe the experimental procedure Nina should follow and how she should process her results.

A

Use the lamp to change the light intensity.
Place lamp at different distances from the leafy shoot
Use metre ruler to measure distance of lamp from leafy shoot
Use at least 4 different distances and use a stopwatch,
Control variables are the same time, control amount of ambient light and control air movement by closing windows.

23
Q

Describe how you can test for the presence of glucose

A

Use Benedicts solution

Look for a red brown precipitate

24
Q

What breaks down starch

A

Amylase breaks down starch. Starch is broken down into sugar.

25
Q

The scientists boils the amylase before using it. Explain the science behind the prediction

A

Prediction - test foe glucose will be negative

Explanation - amylase enzyme has been denatured. It has permanently changed shape and can no longer bind.

26
Q

Plants can control water loss by closing their stomata.
Describe the consequences for photosynthesis for plants living in dry places if they need to close their stomata to save water.

A

Will be unable to take in CO2

So rate of photosynthesis decreases

27
Q

How to prepare specimen before investigation - microscopy

A

Take a clean slide, use a pipette to put out one drop of water or mounting in the middle to make specimen stick
Use tweezers to place specimen on slide
Add drop of stain is specimen is colourless or transparent
Lower the cover slip and make sure no air bubbles are trapped

28
Q

How to view on microscopy

A

Clip the slide to stage
Select lowest powered objective lens
Use coarse adjustment knob to move stage up and down
When focused, adjust fine adjustment knob
If needs to see specimen with greater magnification swap to higher powered objective lens

29
Q

How to experiment starch

A

Amalyases breaksdown starch to maltose
Use iodine solution , if starch is present the iodine solution will change from browny orange to blue black

Repeat with water bath at different temps to see how it affects the time taken

30
Q

How to improve accuracy with the experiment of starch

A

Colorimeter - electronic equipment that measures the strength go the coloured solution so measurements are just based on somebody’s judgement .

31
Q

How to tests for reducing sugars ?

A

Sugars made from just one unit.
Add Benedicts reagent to a sample and heat it in a water bath that has been set to 75 degrees
If positive it will form a coloured precipitate
The higher the concentration the further the colour changes goes

32
Q

How to tests for non reducing sugars?

A

sucrose
Use a sample, add HCL and heat in bath for 75 degrees
add sodium hydrogen carbonate to neutralise , then carry out normal Benedictus test
If colour precipitate non reducing sugar is present

33
Q

What is the test for lipids?

A

Shake the test substance with ethanol, until it dissolves
pour solution into water
if lipids presents milky present

34
Q

What is the test for proteins?

A

Biuret Test
Add a few drops of sodium hydroxide solution to make alkaline
Then add some copper sulphate solution (bright blue)
If protein present solution turns purple

35
Q

How to investigate osmosis?

A

Prepare sucrose solutions of different concentrations ranging from pure water to very concentrated
Cut potato chips to same size
Measure the mass of potatoes
Place in solution
Leave the cylinder in the solution
remove potato and pat dry gently with paper towel
Weigh each again record results

36
Q

What is the only thing you should change in sucrose solution

A

Only thing that changes is sucrose solution concentration

37
Q

How to calculate percentage change

A

Final - initial / initial * 100

38
Q

What would the results for potato chips show

A

In higher water potential ( pure water) , potatoes draw in water by osmosis so become longer and mass increase

In lower water potential ( conctrated) potato will shrink and mass will decrease

39
Q

how to plot graph for osmosis practical

A

y axis - mass in potato

x axis - glucose concentration

40
Q

How to calculate surface area to volume ratio

A

Surface area : length * width * face

volume : length * width * height

41
Q

How to investigate using transect?

A

Mark out a line using a tape measure
Place a quadratic at the start of the line and count and record organisms you find
work along the line
Work out using percentage cover and plot using kite diagram

42
Q

How to investigate antimicrobials

A

Pour hot agar jelly on Petri dish
when jelly cooled, inoculating loops transfer microorganisms
Take three discs of filter paper soak in different antibiotics,
C is control disc
Place discs on jelly and tape lid
Leave dish for 48 hrs at 25 degrees
Let bacteria grow , anywhere it can’t grow is clear zone, it is where antibiotic is effective.

43
Q

What aseptic techniques should be used to not contaminate

A

Disinfect work surfaces using alcohol
sterilise all glassware in an auto clave, including agar jelly
if inoculating loop is used , sterilise in hot flame
work near a Bunsen burner, hot air rises so microbes in the air should be drawn away from the culture.
Briefly flame the neck of the glass container of bacteria just after its opening, to prevent unwanted microbes from falling in.