B2.080 Regulating the Activity of Transcription Factors Flashcards
1
Q
what are the 3 TF domains?
A
DNA binding domain
Dimerization domain
Transcriptional activation domain
2
Q
how do TFs bind to DNA?
A
domain recognizes a specific DNA sequence
hydrogen or electrostatic bonds between specific amino acid residues and nuceotides
3
Q
how many nucleotide sequences does an independent domain typically recognize?
A
3-24
these are called “response elements”
4
Q
what is the most common DNA binding motif?
A
zinc fingers
5
Q
what is the purpose of the dimerization domain?
A
different dimers of proteins can exhibit different DNA binding and transcriptional regulatory activities
6
Q
what is a transcriptional activation domain (TAD)?
A
portion of a transcription factor protein that makes contact with co-activators to alter gene transcription
