B2.030 Overview of Oxidative Metabolism

Question 1

how many net ATP are made in glycolysis?

Answer 1

2

Question 2

how many ATP are made in TCA?

Answer 2

1

Question 3

how many ATP are made in mitochondrial ETC?

Answer 3

24

Question 4

what is the relationship between ETC complexes and redox potential?

Answer 4

each complex has a lower redox potential than the previous one

Question 5

what is the purpose of the ETC?

Answer 5

harvest e- from NADH and FADH to pump protons and create a gradient in the intermitochondrial space

Question 6

which complex makes ATP?

Answer 6

V, via mechanical motion

Question 7

what is the final e- acceptor on the ETC?

Answer 7

O2 –> H2O

Question 8

how are e- harvested?

Answer 8

in glycolysis and TCA by reduction of NADH and FADH

Question 9

what is the overall result of glycolysis?

Answer 9

1 glucose split into two pyruvate
harvests electrons NAD+ to NADH
makes 2 ATP

Question 10

what is the fate of pyruvate when oxygen is present?

Answer 10

oxidized to Acetyl-CoA

CoA feeds TCA

Question 11

what is the fate of pyruvate when oxygen is absent/in RBCs w no mitochondria?

Answer 11

pyruvate reduced to lactate
NADH back to NAD+
NAD+ keeps glycolysis going

Question 12

what is the fate of most lactate produced from pyruvate?

Answer 12

sent via blood to liver where its converted back to pyruvate and then to glucose (Cori cycle)

Question 13

what are the sources of Acetyle-CoA for the TCA?

Answer 13

oxidation of pyruvate, beta-oxidation of fats, breakdown of aas

Question 14

what are the byproducts of the TCA?

Answer 14

1 ATP
3 NADH
1 FADH
2 CO2 (exhaled)

Question 15

which cofactor shuttles e- from complexes 1&2 to 3?

Answer 15

coenzyme Q

Question 16

which cofactor shuttles e- from 3 to 4?

Answer 16

cytochrome c

Question 17

what occurs in the mitochondrial matrix?

Answer 17

TCA

Question 18

what occurs in the intermitochondrial membrane space?

Answer 18

ETC creates proton gradient