B2 Flashcards
Jan 20 See Jul 08 Jul 05
Animal studies can be used to assess acute and chronic effects of hazardous substances.
(a) Outline the meaning of the following terms used in animal studies:
(i) NOAEL; (2)
(ii) LD50. (2)
(b) Outline the advantages and disadvantages of using animal studies to investigate whether a substance may be hazardous to humans. (6)
(a)
(i) NOAEL stands for No Observed Adverse Effect Level and is a level of exposure at which there is no evidence of harm.
(ii) LD50 is an abbreviation of “Lethal Dose 50%” - that is the single oral dose of a substance that is ingested and kills 50 per cent of a test population. LD50 is measured in milligrams (or grams) per kilogram body weight (mg / kg).
(b) Advantages
• Does not rely on exposing people to chemicals, thereby preventing human disease and suffering.
• Can be quicker and cheaper than epidemiological studies on human populations.
• Provides good-quality information about the effects of exposure to certain chemicals that could not be
determined by other types of study, such as in-vitro studies.
• Allows the long-term effects of exposure to low doses to be studied, replicating the actual types of
exposure that workers might experience at work.
Disadvantages
• Responses vary between species, so application to humans may be questionable
• Raises ethical questions about testing on animals
• Diseases like cancer may have a number contributing effects
• Can be expensive/time consuming
Jul 19
(a) Explain how exposure to silica dust can cause silicosis. (4)
(b) The construction of a city’s underground rail line involves extensive tunnelling and concrete spraying activities.
Outline controls that reduce the risk of employees developing silicosis, while carrying out tunnelling and concrete spraying activities. (6)
(a) Once inhaled, the silica dust can be trapped in the alveoli and producing scarring or silicosis. The scarring, or hardening of lung tissue, affects the function of the lungs making breathing difficult. This is a chronic condition with sufferers often finding that symptoms of silicosis can worsen even if exposure ceases.
(b) Control measures: LEV; use of HEPA vacuum (workshop); use of power tools with integral extraction / water spray; wet working - water spray, damping down (workshop & on-site); PPE - eye protection, gloves, RPE (workshop & on-site); washing, changing, clothes / PPE / RPE storage facilities (workshop & on-site).
Jan 19
Human epidemiology and animal studies are methods that can be used to investigate whether a substance is carcinogenic.
(a) (i) Explain what is meant by the term ‘carcinogen’. (2)
(ii) Outline the advantages and disadvantages of human epidemiology. (5)
(iii) Outline the advantages and disadvantages of animal studies. (5)
(i) a carcinogen is a substance or a mixture of substances which induce cancer or increase its incidence It attacks the mechanism which controls reproduction of normal cells and causes changes in the cell which divide uncontrollably. They grow, spread, invade and destroy the surrounding tissue.
(ii) Epidemiology Disadvantages
• Large study populations are needed and over a long period
• Often people are lost from the study as it progresses over time.
• Poor quality of health affects data and/or exposure data
• Such studies are costly.
Epidemiology Advantages
• Is based on actual experience in actual populations
• Can reveal links between a particular chemical and ill health in humans
Definitive method for identifying human carcinogens;
- (iii) Animal studies Disadvantages
- Responses vary between species, so application to humans may be questionable
- Raises ethical questions about testing on animals
- Cost and the amount of time it takes;
- Requires large populations in order to reduce sampling errors;
- Poor or inaccurate historical records;
- Affected by lifestyle factors;
- Not very useful for assessing completely new substances since latency periods may be extensive;
- The study might be affected by synergistic or additive effects.
Jan 19
Human epidemiology and animal studies are methods that can be used to investigate whether a substance is carcinogenic.
(b) Outline the control measures that should be in place in a workplace where a carcinogenic substance is used. (8)
(b) Control measures include:
- Keeping quantities to a minimum
- Total enclosure,
- Use of Local Exhaust Ventilation
- Prohibition of eating and drinking in contaminated areas,
- Designation and cleaning of contaminated areas,
- The use of suitable warning signs
- Closed and labelled containers
- Reducing the number of people that are exposed
- Reducing the time that people are exposed
- Labelling and sealing of any contaminated waste
Jan 17 Jan 14 Jan 11
A facilities manager of a multi-occupancy office block built in the 1970s is concerned about the risks associated with asbestos in the building.
(a) Outline the steps the facilities manager should take to minimise the risks associated with any asbestos that may be present in the building. (12)
(a) The facilities manager should:
accept responsibility as dutyholder in relation to CAR reg 4 “duty to manage asbestos”; assume asbestos present (age of building); carry out / arrange for an asbestos survey of the building by a competent person to identify suspected “asbestos-containing materials” (ACMs); carry out / arrange for (intrusive / destructive) sampling as necessary; survey / sampling to be conducted as per P402 or ABICS accredited); draw up an asbestos management plan; maintain records in an asbestos register; keep records of type, location and condition of ACMs; label asbestos / provide warning signs at appropriate locations; prioritise asbestos management – encapsulate / repair, remove; review and update register periodically and after any significant maintenance activity / disturbance; make register / information available to building occupants, contractors etc
A facilities manager of a multi-occupancy office block built in the 1970s is concerned about the risks associated with asbestos in the building.
(b) An occupant of one of the offices engages a contractor to fit a hand dryer in a toilet which involves drilling through some asbestos insulating board.
Outline the steps that should be taken by the contractor to carry out this work in accordance with the Control of Asbestos Regulations 2012. (8)
(b) The contractor should: carry out this work, in accordance with the Control of Asbestos Regulations 2012.
• Ensure the area is appropriately signed and cordoned off;
• Remove furniture and fittings from the area or protect using 500-gauge polythene sheeting;
• Protect nearby surfaces from contamination by covering with 500-gauge polythene sheeting and fix with duct tape to non-asbestos surface;
• Cover the drill entry and, if accessible, exit points with a generous amount of paste or foam - us a hand drill rather than an electric drill;
• Drill through the paste or foam;
• Clean off the paste, foam and debris with damp rags;
• Seal the drilled edge with sealant;
• Clean the area and equipment with the Class H vacuum cleaner and damp rags;
• Double bag all debris, used rags, paint brushes, polythene sheeting and other waste as dispose of as asbestos waste;
Jul 13 Jan 10 Jan 06
(a) Outline the principles of a prospective cohort study, as used in epidemiology. (4)
(b) Outline factors that may affect the reliability of such cohort studies. (6)
(a) A prospective cohort study is designed to verify or deny a hypothetical link between an occupational exposure and an ill-health condition. It involves following two similar groups (cohorts) forward in time to evaluate their future health outcomes. One of the groups / cohort has been (or is being) currently occupationally exposed to the agent under investigation while the other cohort has not. For example, workers in the micro-electronics industry who are exposed to toxic chemicals are followed forward in time (tracked) to see if there is an excess of cancers arising in future years; their health outcomes can be compared to those for a similar unexposed cohort (a control group).
(b) Reliability of epidemiological cohort studies is affected by:
• inadequacy of the data - health records / death certificates are not very reliable sources of information on occupation / illness / cause of death
• inaccurate recollection of past exposures / occupational history etc
• tendency to tell researchers what they “want to hear” (“now you come to mention it…” = recall bias)
• cohort size - need reasonably large numbers to ensure statistical validity
• difficulties in selecting comparable cohorts
• confounding factors - lifestyle (smoking / diet / exercise etc); social class / income
• long latency periods for certain conditions requires long term studies - delayed results
• difficulty in tracking people / people drop out / move away
• “healthy worker effect” - sick individuals tend to be “selected out” of workforce so cohort is not representative of general population (selection bias)
Jan 13 (a) Outline the advantages and disadvantages of animal testing as a model to predict the effects of hazardous substances on humans. (6)
a) Advantages of animal testing:
- Avoids human exposure and hence possible human risk;
- Data can be collected more quickly than by using epidemiological methods;
- Environment of the animal can be better controlled;
Disadvantages of Animal testing
- The dose/response effect may vary in different animal species (converting data to humans may not always be reliable);
- Time-consuming and expensive dependent on the species used;
- Ethical considerations and public opinion that can make this approach more difficult to undertake;
(b) A principle of REACH legislation is that animal testing should only be done as a last resort.
Outline how each of the following can provide data on the human health effects of hazardous substances without animal testing:
(i) in vitro testing (3)
(ii) read-across (3)
(iii) Quantitative Structure Activity Relationship (QSAR) (2)
(b)
(i) in vitro
• Ames test for mutagenicity;
• Involves experiments carried out in a test tube or petri dish;
• Tissue without histidine are exposed to a substance – the substance is a mutagen if it allows the tissue to grow;
• Involves exposing cells or tissues, grown outside a living organism, to a hazardous substance;
• As a result, an understanding of how that substance was metabolised or affected the cells was gained.
(ii)
• Read-across is based on the assumption that substances with similar chemical structures will have similar toxicological effects on humans, so this approach can be used to avoid or reduce the need for animal testing on new substances that are similar in structure to those already tested;
• Instead the data already recorded for similar substances can be read across to predict the toxicological properties of the new substances.
(iii)
• Uses computer based models to predict the likely effects of substances on humans;
• The predictions are based on chemical structure and can also provide an estimation of the risks in relation to the levels of exposure.
(c) A UK company manufactures 3 tonnes / year of a new hazardous substance for sale in the EU.
Outline the steps a company must take in order to comply with the REACH legislation. (6)
c)
• Quantities of more than 1 tonne make the REACH legislation applicable;
• The substance must be registered with the European Chemicals Agency (ECHA);
• Part of this registration process is to provide technical information on the physiochemical, toxicological and the eco-toxicological properties of the substance;
• A safety data sheet would need to be provided to users;
• If changes were made to the substance or the quantities being manufactured were to increase, then this would need to be notified and further information may have to be provided;
[* only required if manufacturing >10 tonne / year]
Jan 13
The Control of Lead at Work Regulations 2002 require that where the exposure to lead of any employee is, or is likely to be, significant, the employer must ensure that the employee is under medical surveillance by a relevant doctor.
(a) Outline the meaning of the term “significant” in relation to lead exposure. (2)
(b) In an organisation that manufactures lead-acid batteries, some of the employees handle lead oxide powder. These employees take part in biological monitoring to measure their lead-in-blood concentration. The results for two general male employees, X and Y, are shown in the table below.
(i) Explain the relevance of the results, with reference to CLAW. (4)
(ii) On the basis of these results outline the actions the employer should take in relation to employee Y in order to comply with CLAW. (4)
(a).
• where any employee is or is liable to be exposed to a concentration of lead in the atmosphere exceeding half the occupational exposure limit for lead;
• where there is a substantial risk of any employee ingesting lead;
• Where there is a risk of lead being absorbed through the skin.
(b)The employees undergo biological monitoring to measure blood-lead concentrations. The results for two general male employees are shown below:
Male employee Lead-in-blood concentration (μg / dl)
X
55
Y
65
(b)(i)
• X had a blood-lead concentration above the action level but below the suspension level;
• Y had a blood-lead concentration that exceeded the suspension level.
(ii)
• suspend an employee because their blood-lead concentration requires this and a doctor has considered it necessary;
• record this in the employee’s health record;
• check that the doctor has communicated this to the employee;
• review the lead risk assessment and control measures;
• check the health of other employees with similar exposures to lead.
Jul 12 Jan 10 Jul 08
Employees can be exposed to corrosive substances.
(a) Give the meaning of the term “corrosive”. (2)
(b) The data below, for three forms of the same product, is taken from a supplier’s catalogue.
Using the data outline the likely routes of entry AND the effects of exposure when handling EACH of these products.(8)
Prodcode Chemical name / form Conc Physical form
C1. Sodium hydroxide (NaOH). 99.9% Pellets
C2 Sodium hydroxide (NaOH). 97%. Powder
C3 Sodium hydroxide (NaOH) 50% in water Liquid
a) Corrosive = may rapidly destroy living tissue if inhaled, ingested or on contact / splashed onto the body.
(b) All corrosive / irritant:
- C1 is in pellet form, it may be ingested (accidentally) affecting the digestive tract and may contact or penetrate the skin. Additionally, dust from the pellets may be inhaled affecting the respiratory tract;
- C2 being a powder, the most likely route of entry would be via inhalation; therefore, the effects of exposure to C2 would be in the respiratory tract. The powder C2 when airborne could also affect the eyes and skin;
- C3 is in liquid form and though it has the lowest concentration of NaOH, will be likely to splash and affect the skin of the face and hands and the eyes;
Jan 12 Jul 09 Jul 07
(a) Describe the structure of the skin and how hazardous substances may enter the body by this route. (6)
(b) Outline how hazardous substances can enter the body through the skin. (4)
(c) Construction company employees regularly lay floors and use a cement-based levelling compound which contains Chromium VI. Several of the long term employees and one of the new recruits have complained about red and sore skin on their hands.
(i) Explain the possible reasons for the symptoms they are now experiencing. (6)
(ii) Outline control measures that could be used to minimise these symptoms in this situation. (4)
(a) & (b) Skin consists of:
• The skin consists of an outer layer, the epidermis which consists of dead cells that are continually shed. It provides some protection against physical and chemical damage but some hazardous substances, particularly biological agents can enter through cuts, abrasions or puncture wounds;
• Beneath this lies the dermis (living cells) containing blood vessels nerve endings and sweat glands. It is partially permeable to water-soluble and fat-soluble substances such as organic solvents which could then enter the bloodstream.
• Below this layer is the hypodermis layer. It is made up of fat and connective tissue and insulates the body against heat/cold and protects our organs/bones from outside forces, such as pathogens.
(b)• beneath this lies the dermis (living cells) containing blood capillaries, nerve endings, sweat glands; partially permeable to water-soluble and fat-soluble substances eg organic solvents / oils which may permeate the skin and then enter the bloodstream.
• Subcutaneous layer - arteries, veins, fatty tissue
(c)
(i) Reasons for symptoms associated with contact dermatitis include prolonged / frequent contact with irritant(s) - eg cement dust / wet cement containing chromium (VI) salts which are recognised skin sensitisers; sensitising agents initiate an allergic / immune response on exposure; sensitisation can occur rapidly (acute) or arise after prolonged exposure (chronic) by degreasing the skin and progressively undermining the skin’s natural defence and repair mechanisms; exposure to abrasive materials is likely to exacerbate the condition.
(ii) Control measures include:
elimination - use alternative compound not cement-based or substitute with a compound containing less cement / lower concentrations of Cr(VI) salts; provide protective neoprene gloves / gauntlets; provide moisturising hand creams / barrier creams; provide washing / drying facilities; provide training and information on health risks / dermatitis; introduce a skin inspection programme (health surveillance) - self inspection / supervision by appointed person
Jan 12 Jul 08 Jan 07
Human epidemiology and animal studies are methods that can be used to investigate whether a substance is carcinogenic.
(a) Outline the advantages of each of these methods. (3)
(b) Outline the disadvantages of each of these methods. (7)
(a)
Human epidemiology
• Definitive method for identifying human carcinogens;
• Based on experience in actual populations;
• Can be done retrospectively.
Animal studies
• Avoids human exposure and hence possible human risk;
• Data can be collected more quickly than by using epidemiological methods;
• Environment of the animal can be better controlled
(b) The disadvantages of using animal studies to investigate the possible carcinogenic effects to humans are:
Human epidemiology
• Cost and the amount of time it takes;
• Requires large populations in order to reduce sampling errors;
• Poor or inaccurate historical records;
• Affected by lifestyle factors;
• Not very useful for assessing completely new substances since latency periods may be extensive;
• The study might be affected by synergistic or additive effects.
Animal Studies
• The dose/response effect may vary in different animal species (converting data to humans may not always be reliable);
• Time-consuming and expensive dependent on the species used;
• Ethical considerations and public opinion that can make this approach more difficult to undertake;
• Difficulties in converting animal data to humans;
Jul 11 Jan 09
(a) Describe the ways the body may defend itself against inhaled dusts. (12)
(b) A company uses a substance in the form of a powder, which is added directly to a mixing vessel from sacks during the manufacture of paints.
Outline the practical control measures that could be used to minimise exposure during the addition. (8)
(a) Dust larger aerodynamic diameter than 10 microns will lodge in nasal hair and mucous in upper respiratory tract - removed by sneezing, coughing, spitting mucous, sneezing, swallowing; dust particles of 5-10 microns will deposit in the “thoracic” area - trachea, bronchi, bronchioles - removable via ciliary escalator, mucous, ejection etc; the respirable dust - less than 5 microns - will penetrate to the gas-exchange regions of the lung (alveoli) where it may pass across cell membrane into blood stream or deposit initiating an auto-immune response whereby the dust particles are engulfed by phagocytes (white blood cells) which may cause scarring / fibrosis / pneumoconiosis (dependent on physico-chemical properties of the dust); respirable dust may also be transported back to ciliary escalator or lymphatic system.
(b) Apply hierarchy of control (eliminate process not acceptable since colour required) but: change physical form of dye from powder to pellets / granules or liquid to reduce or eliminate dust generation; alternative to sacks = automated / enclosed feed system for dispensing powder into mixing vessel; LEV; spill control measures - HEPA vacuums/ spill containment kits etc; PPE - gloves, overalls, goggles; RPE - FFP / respirator; hygiene arrangements - washing / changing facilities