B1 - Cell Structure and Transport (Y10 - Spring 1) Flashcards
๐ข How do you Calculate the Actual Size and Image Size of a Specimen and also the Magnification?
(Equation and Triangle)
Magnification = Image Size / Actual Size of Specimen
In a Triangle I is at the top, A and M are on the bottom, which creates an โI AMโ triangle.
๐ข Parts of the Microscope:
- Eyepiece -Fine Focus
- Objective Lens -Coarse Focus
- Stage -Fine Focus
- Slide -Light
๐ข Why was the invention of the Electron Microscope so important?
The invention of the electron microscope in the 1930โs allowed biologists to see and understand more about the subcellular structures inside of cells. These instruments use a beam of electrons to form an image and can magnify objects up to around 2 million times. Transmission electron microscopes give 2D images with very high magnification and resolution. Scanning electron microscopes give dramatic 3D images but lower magnifications. (These electron microscopes are very expensive and have to be kept in special temperature, pressure and humidity-controlled rooms).
๐ข How Do You Calculate Magnification?
With a light microscope, you multiply the magnification of the eyepiece lens by the magnification of the objective lens. So if your eyepiece lens is X4, and your objective lens is X10, then you do:
e.g: 4 (Eyepiece Lens) x 10 (Objective Lens) = 40 (Overall Magnification)
๐ข What is Magnification and Resolving Power
There is a minimum distance between two objects to be able to see them as two seperate things; if they are closer than this, they will appear as one object. Resolution is the ability to distinguish between two seperate points and it is the resolving power of a microscope that affects how much detail it can show. A light microscope has a resolving power of about 200nm, a scanning electron microscope of about 10nm and a transmission elelctron microscope of about 0.2nm (that is approximatly the distance apart of two atoms in a sold substance).
๐ข Resolving Power Definition
Resolving Power is measured by its ability to differentiate two lines or points in an object, essentially, being able to tell two seperate cells, or whatever else is being looked at apart.
๐ข What is found in the Structure of an Animal Cell?
- Cell Membrane
- Cytoplasm
- Ribosomes
- Mitochondria
- Nucleus
๐ข What is found in the Structure of an Plant Cell?
- Cell Membrane
- Cytoplasm
- Ribosomes
- Mitochondria
- Nucleus
- Large Permnent Vacuole
- Chloroplasts
๐ข Nucleus Definition
Controls all the activities of the cell and is surrounded by the nuclear membrane. It contains the genes on the chromosomes that carry the instructions for making the peotiens needed to build new cells or new organisms. The average diameter is around 10ยตm.
๐ข Cytoplasm Definition
A liquid gel in which the organelles are suspended and where most of the chemical reactions needed for life.
๐ข Cell Membrane Definition
Controls the passage of substances such as glucose and mineral ions into the cell. It also controls the movement ions into the cell. It also controls the movement of substances such as urea or hormones out of the cell.
๐ข Mitochondria Definition
Structures in the cytoplasm where aerobic respiration takes the place, releasing energy for the cell. They are very small, around 1ยตm - 2ยตm in length and only 0.2-0.7ยตm in the diameter.
๐ข Ribosome Definition
This is where protein synthesis takes place, making all the proteins need in the cell.
๐ข Chloroplast Definition
These are found in tne green parts of a plant. They are green because they contain a substance called chlorophyll, which abosorbs light so the plant can make food photosynthesis. Each chloroplast is around 3-5ยตm long. (Root cells do not have chloroplasts because they do not photosynthesise.
๐ข Large Permanent Vacuole Definition
This is a space in the cytoplasm filled with cell sap. This is important for keeping the cells rigid to support the plant.
๐ข What are Eukaryotic Cells?
Eukaryotic Cells are examples of plant and animal cells. Eukaryotic Cells all have a cell membrane, cytoplasm, and genetic matrial that is enclosed in a nucleus. The genetic material is a chemical called DNA and this forms structures called chromosomes that are contained within the nucleus. All animals (including humnan beings), plants, fungi and protista are Eurkaryotes.
๐ข What are Prokaryotic Cells?
Bacteria are single-celles living organisms. They are examples of prokaryotes. At 0.2 - 2ยตm in length, prokaryotes are 1-2 orders magnitude smaller than Eukaryotes, meaning you could hundreds of thousands of bacteria onto something such as this full stop. Because of this, you cannot se individual bacteria without a very powerful microscope. When you cluture bacteria on an agar p,ate, you grow many millions of bacteria. This enables you to see the bacterial colony with your naked eye.
Bacteria have cytoplasm and a cell membrane surrounded by a cell wall, but the cell wall does not contian the cellulose you see in plant cells. In prokaryotic cells the genetic material is not encolsed in a nucleus. The bacterial chromosome is a single DNA loop found free in the cytoplasm. Prokayotic cells may also contain extra small rings of DNA called plasmids. Plasmids code for very specific features such as antibiotic resistance.
๐ข Adaptations on the Bacteria and how it can be Good/Bad
Some of the bacteria have protective slime capsules around the outside of the cell wall. Some types of bacterium have at least one flagellum (that is a long protein strand that lashes about). These bacteria use their flagella to move themselves around. Many bacteria have little/no affect on organisms but many of them can be very useful despite this. On the other hand, some bactria can be harmful, as they can cause diseases in humans and other animals as well as the fact that they can also decompose and destroy stored food.
๐ข What Came First: Eurkaryotic or Prokaryotic Cell?
This idea of Prokaryotic Cells existing before Eukaryotic Cells is a reasonable idea, and also links in with the theory of how the Earth has evolved and grown since the Universeโs existence. Points to back this fact up are that Eukaryotic Cells are a lot more complex than Prokaryotic Cells, meaning that they could be an evolved version of Prokaryotes. Also, there is a lot less variation in the Prokaryotic Cells, so think could also point towards it being older and less expansive and different. A third point and reason is that Eukaryotic Cells can come from Animals, Plants, Protist and Fungi, which were all developed later on in the evolution of Earth, meaning the Prokaryotic Cells couldโve been able to exist before them.
The first ever Eukaryotic Cells are though to have evolved around 2 billion years ago, whilst Prokaryotic Cells are though to have evolved 3.5 billion years ago. From, this I have also found out that the evolution of the Eukaryotic Cell is explained by the endosymbiotic theory, and that the Prokaryotic Cell were in existence about 1 billion years after the formation of the Earthโs crust, but only appeared in the fossil records later on.
๐ข How is Bacteria Different to Plant and Animal Cells?
Bacteria is different to Animal and Plant Cells in the way that it is structured and they way it reproduces and spreads. For example, Bacteria cells do not have a nucleus meaning their DNA is free-flowing unlike Plant and Animal cells. Also, some types of Bacterium have at least one flagellum, in which Plant and Animals cells always have none.