✅ 5. Required Practical: Food Tests (B3) Flashcards
What is an Independant Variable
In Independant Variable is the variable that is altered during a scientific experiment.
What is a Dependant Variable
A Dependant Varibale is the variable being tested or measured during a scientific experiment.
Wnat is a Control Variable
A Control Variable is the variable that is kept the same during a scientific experiment.
How do you test food for Starch
Method:
1. Place a small amount of food on the spotting tile.
- Add a few drops of iodine solution to the food on the spotting tile.
- Yellow-red iodine solution turns blue-black if starch is present.
- Repeat steps 1-3 for other types of food.
- Record the results in a results table
How do you test for Sugar
Method:
1. Place a small amount of food in a test tube.
- Add enough Benedict’s solution to cover the food
- Place the test tube in a warm water bath for 10 minutes
- Blue Benedict’ solution turns brick red on heating if a sugar such as glucose is present
- Repeat steps 1-4 for other types of food
- Record the results in a results table
How do you test food for Lipids
Method:
1. Place a small amount of food into the test tube.
- Add a few drops of ethanol to the tube
- Shake the test tube and leave for one minute
- Pour the solution into a test tube of water
- Ethanol added to a solution gives a cloudy white layer if a lipid is present.
- Repeat steps 1-5 for other types of food
- Record the results in a results table
How do you test for Protien
Method:
1. Place a small amount of food in a test tube
- Add 1cm^3 of Biuret reagent. Alternatively, add 1cm^3 of sodium hydroxide solution and then add a few drops of copper sulphate solution
- Blue Biuret turns purple if protein is present
- Repeat steps 1-3 for other types of food
- Record the results in a results table
Equipment needed for Food Tests Practical
Equipment:
- A range of small pieces of different foods (e.g cheese, crips, pasta, ham, bread, boiled sweets, nuts e.c.t)
- Test tubes
- Test tube rack
- Spotting tile
- Iodine Solution
- Benedict’s Solution
- Biuret reagent of dilute sodium hydroxide solution and copper sulphate solution
- Disposable pipettes
- Filter paper
- Water bath or beakers and a supply of hot water
- Sticky labels or waterproof pen
Health and Safety for Food Tests Practical
Health and Safety:
- Do not eat any of the food
- Some people may have food allergies
- Wear chemical splash-proof eye protection
- Biuret reagent (IRRITANT)
- Sodium hydroxide (IRRITANT)
- Ethanol (HIGHLY FLAMMABLE), keep away from naked flames
- Iodine solution (HARMFUL), avoid contact with skin
- Water in the water bath will be hot
(To see each setup in more detail, see Goodnotes)
How To Display the Results
A Results Table should be created with 6 columns having the headings of: ‘Food’Reagent’, ‘Food group tested for’, ‘Colour before adding to food’, ‘Colour chnage if food group is present’, and ‘Result (Positive/Negative)’. Then, have as many rows as there is needed, and fill in the table based on the results that are found.