AW L2.2 Vaccine Manufacture

Question 1

4 Critical factors in vaccine manufacture:

Answer 1

• growth potential of seed virus
• timing of strain selection
• potency test reagents
• timing of annual license approval

Question 2

Why does ‘growth potential of seed virus’ limit manufacture?

Answer 2

Trivalent vaccine timing limited by the least productive monovalent strain

Question 3

Why does ‘timing of strain selection’ limit manufacture?

Answer 3

• Prod time limited to necessary of distribution and admin of vaccine before flu season
• working seeds need 4 weeks development min.

Question 4

Why does ‘potency test reagents’ limit manufacture?

Answer 4

• You are required to determine the potency before formulation of vaccine
• Must be standardized for new strains

Question 5

How does vaccine work?

Answer 5

Taken up into phagosome
Processed and broken up into antigens
Presented on MHC receptor
Sticking immune cells
Naive T cells become memory specific T cells