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HEMATOLOGY > AUTOMATED BLOOD CELL ANALYSIS > Flashcards

AUTOMATED BLOOD CELL ANALYSIS Flashcards

1
Q

Most hematology analyzers rely on two basic principles, what are these?

A

o Electronic impedance (resistance)
o Optical scatter

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2
Q

Electronic impedance is AKA?

A

low-voltage direct current (DC)

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3
Q

EP was developed by?

A

Coulter

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4
Q

EP is based on the?

A

detection and measurement of changes in electrical resistance produced by cells

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5
Q

This is based on the detection and measurement of changes in electrical resistance produced by cells

A

ELECTRONIC IMPEDANCE (RESISTANCE)

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6
Q

This uses both lases and nonlaser light

A

Optical scatter

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7
Q

In this method, a ______________ stream is directed through an anode. This process employs _____________, where the sample is passed through a _____________ and illuminated by a ______________, typically a ____________________

A

hydrodynamically focused sample
flow cytometry
quartz flow cell
focused light source
tungsten halogen lamp or a helium-neon laser.

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8
Q

What are the focused light source used in OS?

A

Tungsten halogen lamo
Helium neon laser

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9
Q

Laser light differs from bright field light in several ways, what are these ways?

A

greater intensity, travels in phase, and exhibits lower dispersion

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10
Q

The several ways that the light differs from bright field enables the detection of?

A

laser interference
facilitating the enumeration
differentiation of various cell types.

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11
Q

The forward angle light scatter, which occurs at ____________, correlates with ___________?

A

zero degrees
cell volume

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12
Q

The _______________, which occurs at zero degrees, correlates with cell volume

A

forward angle light scatter

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13
Q

This is another principle that is used usually in conjunction with DC electronic impedance because it provides a higher voltage

A

RADIOFREQUENCY

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14
Q

What to Radiofrequency uses?

A

high-voltage electromagnetic current

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15
Q

The density of the cell interior is _______________ to the pulse height or the change in the radio frequency signal

A

PROPORTIONAL

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16
Q

conductivity measured by radio frequency is correlated with the?

A

✓ nucleus-to-cytoplasm ratio
✓ nuclear density
✓ cytoplasmic granulation

17
Q

The setup for electronic impedance involves ______________ and a __________________. As blood cells pass through
the__________ (a small slit), their type can be determined
based on _________________

A

two electrodes
blood cell suspension
aperture
size and volume

18
Q

The cells are typically suspended in an
___________________, such as saline,
and are drawn through the aperture. When the
two electrodes detect cells passing by, they will
send out ___________, which are used to measure the
characteristics of the _______________?

A

electrically conductive diluent
pulses
cell wall

19
Q

What will determine the height of each voltage pulse?

A

volume of the cell

20
Q

Where is the data produce by the machine is plotted on a volume distribution?

A

histogram

21
Q

relative _______________ is
represented on the y-axis and the _____________ is displayed on
the x-axis

A

number of cells
volume

22
Q

Manufactures an extensive line of hematology analyzers ncluding the smaller Ac-T series that provides complete RBC, platelet and WBC analysis with a five-part differential

A

BECKMAN COULTER, INC

23
Q

what is utilized in Beckman coulter?

A

Electrical impedance

24
Q

Sysmex corporation is AKA?

A

TOA Medical Electronics Company

25
Q

Formerly known as TOA Medical Electronics Company,
Ltd. provides a complete RBC, platelet, and WBC
analysis with a three-part differential

A

SYSMEX CORPORATION

26
Q

is
designed to alert you to differences or alterations in the
size, volume, or types of cells present in a sample

A

flagging system

27
Q

Calibration must be performed initially upon installation
when the instrument arrives in the laboratory and verified at
least ___________________.

A

every six months

HEMATOLOGY (5 decks)

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