AUTOMATED BLOOD CELL ANALYSIS Flashcards
Most hematology analyzers rely on two basic principles, what are these?
o Electronic impedance (resistance)
o Optical scatter
Electronic impedance is AKA?
low-voltage direct current (DC)
EP was developed by?
Coulter
EP is based on the?
detection and measurement of changes in electrical resistance produced by cells
This is based on the detection and measurement of changes in electrical resistance produced by cells
ELECTRONIC IMPEDANCE (RESISTANCE)
This uses both lases and nonlaser light
Optical scatter
In this method, a ______________ stream is directed through an anode. This process employs _____________, where the sample is passed through a _____________ and illuminated by a ______________, typically a ____________________
hydrodynamically focused sample
flow cytometry
quartz flow cell
focused light source
tungsten halogen lamp or a helium-neon laser.
What are the focused light source used in OS?
Tungsten halogen lamo
Helium neon laser
Laser light differs from bright field light in several ways, what are these ways?
greater intensity, travels in phase, and exhibits lower dispersion
The several ways that the light differs from bright field enables the detection of?
laser interference
facilitating the enumeration
differentiation of various cell types.
The forward angle light scatter, which occurs at ____________, correlates with ___________?
zero degrees
cell volume
The _______________, which occurs at zero degrees, correlates with cell volume
forward angle light scatter
This is another principle that is used usually in conjunction with DC electronic impedance because it provides a higher voltage
RADIOFREQUENCY
What to Radiofrequency uses?
high-voltage electromagnetic current
The density of the cell interior is _______________ to the pulse height or the change in the radio frequency signal
PROPORTIONAL
conductivity measured by radio frequency is correlated with the?
✓ nucleus-to-cytoplasm ratio
✓ nuclear density
✓ cytoplasmic granulation
The setup for electronic impedance involves ______________ and a __________________. As blood cells pass through
the__________ (a small slit), their type can be determined
based on _________________
two electrodes
blood cell suspension
aperture
size and volume
The cells are typically suspended in an
___________________, such as saline,
and are drawn through the aperture. When the
two electrodes detect cells passing by, they will
send out ___________, which are used to measure the
characteristics of the _______________?
electrically conductive diluent
pulses
cell wall
What will determine the height of each voltage pulse?
volume of the cell
Where is the data produce by the machine is plotted on a volume distribution?
histogram
relative _______________ is
represented on the y-axis and the _____________ is displayed on
the x-axis
number of cells
volume
Manufactures an extensive line of hematology analyzers ncluding the smaller Ac-T series that provides complete RBC, platelet and WBC analysis with a five-part differential
BECKMAN COULTER, INC
what is utilized in Beckman coulter?
Electrical impedance
Sysmex corporation is AKA?
TOA Medical Electronics Company
Formerly known as TOA Medical Electronics Company,
Ltd. provides a complete RBC, platelet, and WBC
analysis with a three-part differential
SYSMEX CORPORATION
is
designed to alert you to differences or alterations in the
size, volume, or types of cells present in a sample
flagging system
Calibration must be performed initially upon installation
when the instrument arrives in the laboratory and verified at
least ___________________.
every six months
