Autogenous Blood Implant

Question 1

PRF vs PRP?

Answer 1

Second generate platlet concentrate
has higher release of Growth_hormone

Anticoag nadare

Question 2

(Note) PRP All about platelet and Gf

Answer 2

By anticoag an centrifuge

Question 3

20 years ago (planet) and (GF)
PDGF as key regulators during (?)
so aim was concentrate on these cells (using)
(?) and (?) so we call this phenomenon (?)/

Answer 3

wound healing
Anticoagulant
centrifuge device.
PRF

Question 4

ADV of plat let concentrates.

Answer 4

• Autogenous tissue (Gold standard)
• Revascalarisation of tissue (Angiogenesis increase
• Recruiting various cell including stem cell
• proliferation (increase)

Question 5

stage of wound healing?

Answer 5

I-P-R
informatory phase
proliferative phase
regenerative phase

Question 6

informatory phase cells are contain:

Answer 6

Cytokines + (?) + blood coagulation + (?)
- Planets
- Growth factor

Question 7

Informatory phase happen (?) after injury-

Answer 7

24-48 h

Question 8

proliferative phase happen (?) after injury

Answer 8

3 days

Question 9

proliferative phase cell’s is (?)

Answer 9

fibroblast

Question 10

process of (?) happen in proliferative phase

Answer 10

• Angiogenesis

Question 11

process of (?) happen in Regenerative.

Answer 11

• tissue regeneration

Question 12

Regenerative phase cells?

Answer 12

• Newly organized Collagen fiber.

Question 13

Role of planet in wound healing?

Answer 13

• key role in WH
  -growth, Angiogenic factors, releasing
  cytokine
• low density

Question 14

Role of white blood cell in WH?

Answer 14

• key role in WH
  -growth, Angiogenic factors, releasing
  cytokine
• low density

Question 15

Role of white blood cell in WH?

Answer 15

• Host defence
• Regenerative phase of healing
• Leukocyte
• high density
• Some to the RBCs
• hard to separate

Question 16

What is PRP?

Answer 16

• high accumulation of plat let and growth
  factor after centrifugation

Question 17

How reach to prp?

Answer 17

• 30-60 minute centrifugation
• Use of anticoagulants together

Question 18

Dis. ADV of PRP.

Answer 18

• long production time
• Use of anticoagulant (stop blood clotting
  before application)
• Liquid consistence.

Question 19

(Exam) during PRP and centrifuge why do we
use of anticoagulant?

Answer 19

• to stop blood clotting before application

Question 20

PRF First ADT?

Answer 20

• Less centrifuge time
• No anticoagulant adding
• Get consistence
• longer GF release profile

Question 21

PRF composition? (not officially)

Answer 21

• No only GF and platlet But also (scafold)
• leukocyte between the Fibrin clot and red
  corpuscles layer.

Question 22

what is scaffold?

Answer 22

Scaffolds, typically made of polymeric biomaterials, provide the structural support for cell attachment and subsequent tissue development.

Question 23

Question 24

PRF composition (officially)

Answer 24

• Bioactive molecule (PDGF, TGF. B, VEGF, EGÉ
  /
• provisional extracellular matrix/3D scaffold): Fibrin matrix •.
  Fibronectin, vitro nectin
  cell Type: Leukocyte_red blood cell

Question 25

(not officially/+ (officially) = Scaffold

Answer 25

(3D scaffold) provisional extracellular matrix
leukocyte between the Fibrin clot and red
corpuscles layer. OR

Fibrin matrix
Fibronectin
vitro nectin
in the
Leukocyte_red blood cell

Question 26

production of PRF

Answer 26

u Fixed-angle centrifugation technique (45 degree )
u Horizontal centrifugation technique

Question 27

Forms of PRF?

Answer 27

u PRF membrane
u PRF plug
u „Sticky bone”
u infiltrative i-PRF (liquid)
u E-PRF membrane

Question 28

protocols for PRF

Answer 28

L-PRF
A- PRF
1- PRF
H-PRF
C- PRF

Question 29

PRF membrane, plug

Answer 29

• Red tube
• te cap should be removed as
  quick as possible after centrifugation
• Remove and discard the red Corpus cels
  base
• Application the deeper instrument.