ATP Generation from Glucose

Question 1

How many NADH are made in each cycle of glycolysis?
How much ATP will these
NADH generate in the ETC/OP?

Answer 1

2 NADH are made in each glycolysis.
These NADH are produced in the cytosol (location of glycolysis), so to enter the mitochondria to start the ATP generation process they are first converted into FADH2.

Overall: 2 FADH2 are made = 3 ATP

Question 2

What is the net yield of ATP from each glycolysis process?

Answer 2

Overall: 2ATP

Question 3

In preparation for the CAC, two pyruvate (from glycolysis) are converted to two Acetyl
CoA.
How many NADH does this reaction generate?
How much ATP will these NADH generate in the ETC/OP?

Answer 3

Each time a pyruvate is converted into an acetyl CoA, an NAD+ is converted into
NADH (happens in the same reaction).
This reaction occurs twice as two pyruvate are made in glycolysis.
This reaction occurs in the mitochondria so the NADH don’t need to be converted into FADH2.

Overall: 2 NADH = 5 ATP

Question 4

Each of the two acetyl CoA are used in one complete CAC reaction.
How many NADH
and FADH2 are made in each cycle of the CAC?
How much ATP will these generate in the ETC/OP?

Answer 4

In each CAC 1 FADH2 and 3 NADH coenzymes are generated.
Two CAC take place as there are two acetyl CoA to use as inputs.

Overall: 6 NADH + 2 FADH2 = 18 ATP

Question 5

What is the net yield of GTP (ATP equivalent) from each CAC?

Answer 5

In each CAC 1 GTP is made. Two CAC take place as there are two acetyl CoA to use
as inputs.