Atherosclerosis : Pathophysiology of Ischaemia + Infarction Flashcards
<p>What is meant by hypoxia?</p>
<p>•Relative lack of blood supply to tissue/organ leading to inadequate O2 supply to meet needs of tissue/organ: hypoxia</p>
<p>What is hypoxic hypoxia?</p>
<p>(a) Low inspired O2 level</p>
<p>(b) Normal inspired O2 but low PaO2 – impairment of diffusion.</p>
<p>What is anaemic hypoxia?</p>
<p>•Normal inspired O2 but blood abnormal</p>
<p>What is stagnant hypoxia?</p>
<p>•Normal inspired O2 but abnormal delivery</p>
<p>(a)Local e.g. occlusion of vessel</p>
<p>(b)Systemic e.g. shock</p>
<p>What is cytotoxic hypoxia?</p>
<p>•Normal inspired O2 but abnormal at tissue level – Something not working with oxygen delivery to the cells</p>
<p>What are the factors afecting oxygen supply?</p>
<p>1. Inspired O2</p>
<p>2. Pulmonary function</p>
<p>3. Blood constituents (haemoglobin)</p>
<p>4. Blood flow (hypotension impairs supply)</p>
<p>5. Integrity of vasculature (atheroma, thrombus/embolus)</p>
<p>6. Tissue mechanisms – deliver oxygen to respiring organelles</p>
<p>What are the factors affecting oxygen demand?</p>
<p>Tissue itself - different tissues have different requirements – fat, bone, connective tissue have lower demand than brain and heart</p>
<p>Activity of tissue above baseline value</p>
<p>What are the two mechanisms for heart ischaemia?</p>
<p>Supply malfunction</p>
<p>Demand Malfunction</p>
<p>Describe supply issues that can cause heart ischaemia</p>
<p>Coronary artery atheroma</p>
<p>Cardiac failure</p>
<p>Pulmonary function - disease and pulmonary oedema</p>
<p>Anaemia</p>
<p>Previous MI</p>
<p>Where is the localised accumulation of lipid and fibrous tissue in atheroma?</p>
<p>In the intima of the arteries</p>
<p>What is the link between atheroma and:</p>
<ul> <li>Stable angina</li> <li>Unstable angina</li> <li>Thrombosis <ul> <li>Aneurysm</li> </ul> </li></ul>
<p>Stable angina - <u>established</u> atheroma in the coronary artery - pain on excertion</p>
<p>Unstable angina - <u>complicated</u> atheroma in coronary artery</p>
<p>Thrombosis - Ulcerated/fissured plaques - thrombosis - ischaemia and infarction</p>
<p>Aneurysm - Atheroma in aorta - walls of aorta become weakened and dilate</p>
<p>What are the clinical consequences of atheroma?</p>
<p>MI</p>
<p>Transient ischaemic attack</p>
<p>Cerebral infarction</p>
<p>Abdominal aortic aneurysm</p>
<p>Peripheral vascular disease</p>
<p>Cardiac failure</p>
<p>Coronary artery disease - MI - Cardiac failure</p>
<p>What are the</p>
<ul> <li>Functional</li> <li>General</li> <li>Biochemical</li> <li>Cellular</li> <li>Clinical</li></ul>
<p>Effects of Ischaemia?</p>
<p><strong>Functional</strong> - Blood/O2 supply fails to meet demands of tissue - (can be because of a reduction in supply or an increase in demand)</p>
<p><strong>General</strong></p>
<p>•Acute</p>
<p>•Chronic - claudication</p>
<p>•Acute-on-chronic – sudden worsening of an already chronic condition</p>
<p><strong>Biochemical</strong></p>
<p>Results in Lactate production -</p>
<p>Lactate to pyruvate takes energy</p>
<p>Lactate can cause death of the cell</p>
<p><strong>Cellular</strong></p>
<p>Variable susceptibility to O2 depending on tissue type and the metabolic rate</p>
<p><strong>Clinical</strong></p>
<p>Dysfunction</p>
<p>Pain</p>
<p>Physical damage to specialised cells</p>
<p></p>
<p></p>
<p>What are the outcomes of Ischaemia?</p>
<p>No clinical effect</p>
<p>Resolution / therapeutic intervention</p>
<p>Infarction</p>
<p>What is meant by infarction?</p>
<p>•Ischaemic necrosis within a tissue/organ in living body produced by occlusion of either the arterial supply or venous drainage</p>
<p>What are the possible aetiologies for infarction?</p>
<p>1. Thrombosis</p>
<p>2. Embolism</p>
<p>3. Strangulation e.g. gut</p>
<p>4. Trauma - cut/ruptured vessel</p>
<p>What does the scale of damage of ischamia/infarction depend on?</p>
<p>1.Time period</p>
<p>2.Tissue/organ</p>
<p>3.Pattern of blood supply (consider collateral circulation – whereby one branch can still supply cells if one is blocked)</p>
<p>4.Previous disease</p>
<p>What is the mechanism of tissue breakdown caused by infarction?</p>
<p>Anaerobic metabolism - cell death - liberation of enzymes</p>
<p>What are the two types of necrosis?</p>
<p>Coagulative</p>
<p>Colliquitive</p>
<p>Where do you find coagulative necrosis?</p>
<p>Heart, lung (most organs)</p>
<p>What is coagulative ischaemia?</p>
<p>Typically caused by ischaemia or infarction</p>
<p>Architecture of the dead tissue is preserved for at least a coupls of days.</p>
<p></p>
<p>Injury denatures structural proteins and lysosomal enzymes - blocks proteolysis of damaged cells - maintains coagulated morphology.</p>
<p>What is liquefactive necrosis/colliquitive necrosis?</p>
<p>Necrosis which results in a transformation of the tissue into a liquid viscous mass. Affected cell is completely digested by hydrolytic enzymes - circumscribed lesion of pus and fluid remains of necrotic tissue - debris is removed by white blood cells and a fluid space is left</p>
<p>How does myocyte death occur as a result of infarction?</p>
<div>•Coronary arterial obstruction leads to a decreasedblood flow to region of myocardium. Results inischaemia, rapid myocardial dysfunction andmyocyte death</div>
<p>What happens within seconds of myocardial ischaemia?</p>
<p>•Anaerobic metabolism, onset of ATP depletion</p>
What happens in under two minutes of myocardial ischaemia?
•Loss of myocardial contractility (heart failure)
What happens within a few minutes of myocardial ischaemia?
•Ultrastructural changes (myofibrillar relaxation, glycogen depletion, cell and mitochondrial swelling) ?reversible
How long does it take for ischaemia to cause irreversible damage to the heart?
20-30 minutes
•Myocyte necrosis (disruption of integrity of sarcolemmal membrane leading to the leakage of intracellular macromolecules: blood tests) - Troponin?
•Injury to the microvasculature - over 1 hour
What do areas of infarction look like less than 24 hours after insult?
•No change on visual inspection
•A few hours to 12 hours post insult, see swollen mitochondria on Electron Microscopy
What organs do you see a plae infarct between 24 and 48 hours?
What organs show a red infarct?
Lung and the liver
Loose tissues, previously congested tissue; second/continuing blood supply, venous occlusion
What can you see microscopically between 24 and 48 hours after infarction?
•Acute inflammation initially at edge of infarct; loss of specialised cell features
What changes do you see in a pale and a red infarct about 72 hours after infarction?
Pale - yellow/white and red rim periphery
Red infarct - little change
•Microscopically: chronic inflammation; macrophages remove debris; granulation tissue; fibrosis
What is the end result of infarction?
•Scar replaces area of tissue damage
•Shape depends on territory of occluded vessel
•Reperfusion Injury – damage when blood supply is restored – after period of ischaemia – inflammation and damage from free radicals
What is the reparative process of myocardial infarction?
•Cell death
•Acute inflammation
•Macrophage phagocytosis of dead cells
•Granulation tissue – new vessel formation
•Collagen deposition (fibrosis)
•Scar formation
What happens after 4-12 hours of myocardial infarction?
•Early coagulation necrosis, oedema, haemorrhage
What happens 12-24 hours after myocardial infarction?
•Ongoing coagulation necrosis, myocyte changes, early neutrophilic infiltrate
What happens 1-3 days after myocardial infarction?
•Coagulation necrosis, loss of nuclei and striations, brisk neutrophilic infiltrate
What happens between 3-7 days of myocardial infarction?
•Disintegration of dead myofibres, dying neutrophils, early phagocytosis
What happens during 7-10 days of myocardial infarction?
•Well developed phagocytosis, granulation tissue at margins (red rim if pale infarct)
What happens between days 10-14 of myocardial infarction?
Well established granulation tissue with new blood vessels and collagen
What happens during weeks 2- 8 after myocardial infarction?
•Increased collagen deposition, decreased cellularity
What happens over 2 months of myocardial infarction?
•Dense collagenous scar
What is meant by a transmural infarction?
ischaemic necrosis affects full thickness of the myocardium
What is meant by a subendocardial infarction?
•ischaemic necrosis mostly limited to a zone of myocardium under the endocardial lining of the heart – just under the endocardial surface
What are the histological features of transmural and subendocardial infarction?
How are acute infarcts clasified?
•according to whether there is elevation of the ST segment on the ECG
What are the features of a NSTEMI?
(non st elevating myocardial infarction)
no ST segment elevation but a significantly elevated serum troponin level
What does a non-stemi correlate with?
subendocardial infarct
What do the effects of infarction depend?
Site
Size of infarct
Death and dysfunction (pain)
Contribution of previous disease and infarction
What are the complications of myocardial infarction?
•Immediate; early; late
•Sudden death; arrhythmias; angina; cardiac failure; cardiac rupture - ventricular wall, septum, papillary muscle; reinfarction; pericarditis; pulmonary embolism secondary to DVT; papillary muscle dysfunction - necrosis/rupture leads to mitral incompetence; mural thrombosis; ventricular aneurysm; Dressler's syndrome
