aseptic technique practical

Question 1

step 1 Spray the bench with……………………..

light…………

Answer 1

disinfectant

light Bunsen burner

Question 2

step 2 label plate around…………

and wash………….

Answer 2

label plate around the outer edge on bottom part

wash hands

Question 3

step 3 Flame ……….. of bottle

Answer 3

neck of McCartney bottle

Question 4

step 4 Remove ……. from ………… culture

Answer 4

0.3 cm cubed

bacterial culture

Question 5

step 5 remove sterile ,,,,,,,,,,, and attach filler

Answer 5

pipette

Question 6

step 6 Lift lid of agar plate at……………

with right hand add……….

Answer 6

angle facing Bunsen burner with your left hand

with right add contents of the pipette onto agar surface

Question 7

step 7 Replace ………..

and place pipette into …………

Answer 7

lid

disinfectant

Question 8

step 8 Take glass spreader , place in …………. and ………… in bunsen

facing Bunsen lift the the lid of agar and …………….

Answer 8

evenly spreads the bacterial culture onto agar

Question 9

step 9 Replace ……….

and add glass spreader to …………

Answer 9

lid and add glass spreader to disinfectant

Question 10

step 10 Take pre-prepared multodisk and transfer……

ensure disk is ,,,,,,,,,,,

add forceps to…………..

Answer 10

to agar plate

flat on agar

disinfectant

Question 11

step 11 Tape agar plate

place it in ……

Answer 11

25 degrees incubator