Aseptic Technique Flashcards
refers to a procedure that is
performed under sterile conditions.
Aseptic technique
This includes medical and laboratory techniques
which deal with cultures and human cells and
tissue for transplantation.
ASEPTIC TECHNIQUE
What is the Aim of Aseptic
Technique?
To prevent the access of micro-organisms
during the preparation and testing.
SOURCES OF CONTAMINATION
- The Atmosphere
- The Breath
- The Hands
- Clothing
- The Hair
- The Working Surface
- Equipment
It is the process by which article, surface or medium is
made free from all microorganisms either in the
vegetative or spore state.
Sterilization
It is the process by which an article, surface or medium is
made free from all pathogenic microorganisms (that is
organisms that are capable of giving rise to infection).
Disinfection
Prevention of infection by inhibiting or arresting the growth
and multiplication of infectious agents.
Grk: anti-against; sepsis-against putrefaction
Antisepsis
What need to be sterilized in the process
of aseptic technique?
- Culture media
- Fluids used in the labs
- Reagents
- Laboratory containers
- Laboratory equipment
Methods of sterilization
A-Physical methods
B. Chemical methods
Physical methods
Dry heat
Moist heat
Filtration
Radiation
Dry heat
i-hot air oven
glassware
ii-flaming
inoculation loop &needle
Moist heat(steam under high pressure):
autoclave —–culture media
Filtration
used for heat sensitive liquids like, antibiotic
solutions and sera.
Chemical methods
Alcohols
aldehydes
Phenolics
Radiation
used for enclosed area such as inoculation room
and virus labs.
