Artificial Insemination

Question 1

Whys use AI?

Answer 1

• breed more mares from one ejaculate
• enable stallions to continue complete
• geographicaL
• reduced injury risk
  -reduces STD risk
• allows for regular seaman evaluation
• sperm can be preserved after death

Question 2

How do you use artificial vaginas for seamen collection?

Answer 2

• serialise equipment (including collection bottle)
  -insert liner
• fill rubber liner with warm water (48-50)
• attach collection bottle to liner
• wear long arm glove and add lune, keep glove on for whole collection.
• check temp with thermometer

Question 3

What are the immediate steps of seamen handling?

Answer 3

• remove gel fraction
• sample and assess raw seamen
• dilute seamen in 1:1 with extender to form stock seamen

Question 4

What are the functions of seamen extender?

Answer 4

• dilute SP to preserve sperm
• buffers pH, isotonic to sperm, neutralises toxins, stabilises enzyme systems
• may include antibiotic

Question 5

Why would a centrifuge extended sperm within a colloid be beneficial?

Answer 5

• good motility
• membrane integrity
• normal morphology
• intact chromatin

Question 6

What are seamen assessed for?

Answer 6

• seamen volume
• volume motility
• sperm concentration in stock

Question 7

How is sperm concentration calculated?

Answer 7

(number counted x 5) x dilution factor x 10000

Question 8

How is raw seamen assessed?

Answer 8

• Morphology = count normal vs abnormal sprem
• Live vs dead ration

Question 9

What is further dilution necessary for?

Answer 9

• dividing seamen in smaller doses
• Fresh AI not needed for a few hours
• for chilled seamen

Question 10

What is the standard insemination concentration used at AI =

Answer 10

50 million perm/ml

Question 11

What is the standard insemination dose?

Answer 11

500 million PMS

Question 12

How to calculate desired volume of seamen to package?

Answer 12

desired number of sperm/ (concentration of extended seamen x PMS)

Question 13

How should the mare be prepared for AI?

Answer 13

• check paperwork is in order
• have ready, sterile insemination, catheter and rubber- free seringe
• check for ovulation within 24 hours
• restrain in stocks
• wrap tail
• clean perinium with warm water and a paper towel followed by diluted hibiscrub

Question 14

What is the process of inseminating the mare?

Answer 14

• draw up whole dose into the seringe
• put on a sterile bottle
• connect syringe to insemination pipete
• insert hand find cervix, put two three fingers slide in the insemination pipete into the uterus
• depress plunger
• leave the pipette in uterus detach seine draw up air then force air through the pipetee