Antisepsis & Sterilization. Flashcards

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1
Q

Germ theory is

A

Germs (microorganisms) cause disease.

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2
Q

Kochs postulates (Robert Koch) are (4)

A

Microbes in all disease
Microbe must be isolate and cultured.
Insert pure microbe, get same disease.
Microbe then can be taken from purposely infected host.

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3
Q

Hospital infections are what? After 48 hours of hospitalization.

A

Nosocomial infection

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4
Q

Other forms of nosocomial infections

A

6-12 months antibiotic use.
Catheters.
Dialysis

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5
Q

What is the best way to limit nosocomial infection?

A

Hand washing

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6
Q

Whats the difference between a disinfectant and a antiseptic?

A

Disinfectant: kill on inanimate
Antiseptic: kill on animate

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7
Q

If antibacterial agents inhibit growth, they’re called

A

Bacteriostatic

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8
Q

If agents destroy bacteria they called

A

Bacterialcidal.

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9
Q

Phenolics and alcohols do what? Against whom?

A

Denature protein and bust up membrane.

Spores, protozoan, non eveloped viruses.

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10
Q

Chlorhexidine does what

A

Alters membrane permeability.

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11
Q

What is more effective than Phenolics and alcohols?

A

Halogen

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12
Q

Halogens do what

A

Bacteria, spores, and viruses

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13
Q

Sulfactants kill by doing what’s

A

Kill cell membrane with philic phobic type compounds.

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14
Q

What is sterilization? Hows it done?

A

Complete removal of all bacteria.

Heat cold filter or radiation.

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15
Q

Autoclaving uses what two things?

A

Heat and pressure.

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16
Q

Dry heat or wet heat kill better?

A

Moist.

17
Q

Boiling kills everything except?

A

Spores and cysts

18
Q

Pasteurization is what?

A

Heat to 55-75 C for 30 min. and cool rapidly. 90% killed (not spores)

19
Q

What radiation is used to sterilize?

A

Non ionizing radiation such as UV. makes thymine thymine diners in DNA.