Antisepsis & Sterilization. Flashcards
Germ theory is
Germs (microorganisms) cause disease.
Kochs postulates (Robert Koch) are (4)
Microbes in all disease
Microbe must be isolate and cultured.
Insert pure microbe, get same disease.
Microbe then can be taken from purposely infected host.
Hospital infections are what? After 48 hours of hospitalization.
Nosocomial infection
Other forms of nosocomial infections
6-12 months antibiotic use.
Catheters.
Dialysis
What is the best way to limit nosocomial infection?
Hand washing
Whats the difference between a disinfectant and a antiseptic?
Disinfectant: kill on inanimate
Antiseptic: kill on animate
If antibacterial agents inhibit growth, they’re called
Bacteriostatic
If agents destroy bacteria they called
Bacterialcidal.
Phenolics and alcohols do what? Against whom?
Denature protein and bust up membrane.
Spores, protozoan, non eveloped viruses.
Chlorhexidine does what
Alters membrane permeability.
What is more effective than Phenolics and alcohols?
Halogen
Halogens do what
Bacteria, spores, and viruses
Sulfactants kill by doing what’s
Kill cell membrane with philic phobic type compounds.
What is sterilization? Hows it done?
Complete removal of all bacteria.
Heat cold filter or radiation.
Autoclaving uses what two things?
Heat and pressure.
Dry heat or wet heat kill better?
Moist.
Boiling kills everything except?
Spores and cysts
Pasteurization is what?
Heat to 55-75 C for 30 min. and cool rapidly. 90% killed (not spores)
What radiation is used to sterilize?
Non ionizing radiation such as UV. makes thymine thymine diners in DNA.
