Anticoagulant

Question 1

EDTA Optimal anticoagulant concentration?

Answer 1

1.5 mg per mL of bood

Question 2

CBC must be analyzed within ___________ if stored in Room temperature

Answer 2

6hrs

Question 3

CBC must analyze within _______ if stored at 4 degrees cel

Answer 3

24 hrs

Question 4

Blood smear should be made within ______ hrs of collection

Answer 4

3hrs

Question 5

Effect of insufficient EDTA

Answer 5

Presence of Clot

Question 6

Effect of Excessive EDTA

Answer 6

⬇️ Hct, ⬇️ESR, Degenerative changes in WBC, ⬆️MCHC, ⬆️Plt ct

Question 7

Anticoagulant used in OFT

Answer 7

Heparin

Question 8

Optimal anticoagulant concentration of HEPARIN

Answer 8

15-20 Units/mL of blood

Question 9

Heparin causes cellular clumping (especially platelets) which leads to _________ and ________

Answer 9

pseudoleukocytosis
(falsely ↑ WBC count) and pseudothrombocytopenia (falsely ↓ platelet count)

Question 10

Sodium citrate, ratio between anticoagulant and blood: _________

Answer 10

1:9

Question 11

FACTORS THAT AFFECT COAGULATION TEST RESULTS:
SHORTENED COAGULATION RESULTS (clue: HEPEP)

Answer 11

Hemolysis
Excessive agitation
Prolonged tourniquet application
Excessive needle manipulation
Platelet contamination

Question 12

FACTORS THAT AFFECT COAGULATION TEST RESULTS: PROLONGED COAGULATION TEST RESULTS

Answer 12

• Presence of clot
• Increased AC conc

Question 13

Black tube is ______
a. 3.2 sodium citrate
b. 3.8 sodium citrate
c. EDTA
d. Heparin

Answer 13

b. 3.8 sodium citrate

Question 14

What tube is used for Westergren and ESR?

Answer 14

Black top (3.8 sodium citrate)

Question 15

What tube is used for the ff. test
* Coagulation Test
* Used also for Platelets function assay (ex. Platelets factor 4 and B-thromboglobulin assays)

Answer 15

Light blue CTAD (citrate, theophylline,
adenosine, dipyridamole)

Question 16

CRITICAL VALUE FOR HEMATOCRIT
a. <21% or <0.21 and >65% or >0.65
b. <22 % or <0.22 and >75% or >0.75
c. <25% or <0.25 and >75% or >0.75
d. <30% or <0.30 and >75% or >0.75

Answer 16

a. <21% or <0.21 and >65% or >0.65

Question 17

CRITICAL VALUE for Hemoglobin
a. <80 g/L and >200g/L
b. <80 g/dL and >200g/dL
c. <70 g/L and >200g/L
d. <80 g/dL and >200g/dL

Answer 17

c. <70 g/L and >200g/L

Question 18

CRITICAL VALUE for Reticulocyte
a. >20%
b. >25 g/L
c. > 25%
d. >20 g/L

Answer 18

a. >20%

Question 19

CRITICAL VALUE for WBC

Answer 19

• < 2,000/uL on a new patient or a 1,000
  difference from
  previous, if less than 4,000/uL
• > 50,000/uL on a new patient

Question 20

Critical value for Platelets <_________/uL and >____ million/uL

Answer 20

< 20,000/uL and NOT previously reported

> 1 million/uL

Question 21

Critical value for Prothrombin time
a. >20 sec
b. >30 sec
c. > 40 sec
d. > 50 sec

Answer 21

c. > 40 sec