Antibody

Question 1

H chain - define

Answer 1

heavy chain - 1 variable region 3-4 constant region

Question 2

L chain - define

Answer 2

light chain - 1 variable region 1 constant region

Question 3

Kappa chains - define

Answer 3

Type of L chain

Question 4

Lamda chains - define

Answer 4

type of L chain

Question 5

Fab - define

Answer 5

Fragment antibody - contains the variable L/H + CL/H (only one control domain

Question 6

Fc - define

Answer 6

Fragment constant - only the heavy chains’ CH2, 3, (4)

Question 7

Put in order on electrophretic separation:

Anode, cathode, albumin, alpha 1, alpha 2, beta, gamma peaks

Answer 7

Cathode | albumin, alpha 1, alpha 2, beta 1, beta 2, gamma | Anode

Question 8

Heavy chain for IgG, IgE, IgD, IgA, IgM

Answer 8

G - 2 gamma chains
E - 2 epsiolon CH4 (4th domain) - affinity for mast cells (histamine) - allergies
D - 2 delta chains for baby cells - receptor molecule - note extra long hinge
A: 4 alpha chains, 1 J, and 1 S.C. chain
M: 10 mu, 1 J ring/chain

Question 9

Hinge

Answer 9

Between Fab/Fc - can change conformation

Question 10

Ig_ has secretory chain

Answer 10

IgA

Question 11

Order immunoglobins classes from largest to smallest

Answer 11

M, A, E, D, G

Question 12

Antibody combining site

Answer 12

3 CDR on each light/heavy chains

Once bound –> allosteric changes. Fc changes structurally

Question 13

CDR

Answer 13

complementary determining region - also hypervariable region - random splicing

Question 14

Allotype

Answer 14

minor allectic difference in sequence of immunoglobulins (ie. Ala vs Tyr on specific spot on heavy chain)

Question 15

Idiotype

Answer 15

antibody with unique CDR - if sequence is recognized by another antibody- will behave as an antigen (Ab behaves as an Ag)

Question 16

IgG is ___ valent?

Answer 16

Di

Question 17

Immune complex can happen when:

Answer 17

antibody cross linking two antigen

Question 18

Aggregation –> fall out of solution. Antigen is a molecule, aggregation is called ______; antigen is a cell, aggregation is called a ____

Answer 18

precipitation, agglutination

Question 19

Ig_ is made by fetus

Answer 19

M

Question 20

Ig_ cross placenta via _________________

Answer 20

IgG –> placenta via active transport

Question 21

Ig_ is decavalent, can activate complement with # antibody

Answer 21

M

1

Question 22

Ig_ needs __ antibody to complement

Answer 22

G, 2

Question 23

IgG has half life of…

Answer 23

3 weeks

Question 24

Phagocytic cells have FcR for Ig_ –> opsonizing

Answer 24

IgG

Question 25

Ig_ is most common in blood

Answer 25

G

Question 26

Ig_ in mucosa

Answer 26

A

Question 27

Ig_ is first line of immunological defense

Answer 27

A

Question 28

Ig_ for B cell receptors

Answer 28

D

Question 29

Ig_ is responsible allergies/hypersensitivity

Answer 29

E

Question 30

Ig_ is for ____ cells

Answer 30

mast/basophils (have FcR for IgE) --> histamines

Question 31

precipitation of antigen/antibody will only form if they are _______ because of ______

Answer 31

Equal in concentration | Precipitation/agglutination

Question 32

Complement for innate and adaptive immunity

Answer 32

Innate - lectin | Adaptive - classical

Question 33

Order of complement via classical

Answer 33

C1q --> 4, 2, 3, 5,6,7,8,9

Question 34

Order of alternative complement

Answer 34

Cell wall = P P --> stablize C3b and anchor for factor B and D --> C3BDbC3b --> C5 --> 6,7,8,9 Autoimmunity

Question 35

Lectin pathway - order

Answer 35

MBP is a lectin - binds foreign carbs | MBP --> Carbs --> 4,2,3,5.6,7,8,9

Question 36

C_ and C_ does lytic

Answer 36

8,9

Question 37

which complement components opsoninze?

Answer 37

C3b; | IgG opsonize for FcR of phagocytes

Question 38

Chemotatic complement component

Answer 38

C5a

Question 39

Anaphylatoxic complement component

Answer 39

C3a, C4a, C5a --> release histamine from mast/basophil --> increase inflammation --> increase blood flow

Question 40

Lysis important for what family of bacterial

Answer 40

Neisseria (gonorrhrea + meningitis) | Reisstant for lysis --> phaogcytosed