Antibodies 3/3 Flashcards
Major function/property of which Ig is Opsonization
IgG1,3
Major function/property of which Ig is Sensitization for killing NK cells
IgG1,3
Major function/property of which Ig is Sensitization of mast cells
IgE
Major function/property of which Ig is Sensitization of basophils
IgD,IgE
Major function/property of which Ig is Activation of complement system
IgM,IgG3,1
Major function/property of which Ig is transport across epithelium
IgA (dimer)
Major function/property of which Ig is Transport across placenta
IgG1,3,4
Major function/property of which Ig is Diffusion into extravascular sites
IgG1,2,3,4,IgA (monomer)
Antigen-Antibody Interaction : Affinity
“initial force of attraction that exists between a single Fab site on an AB molecule and a single epitope on the AG
- allows ABs to stick to AG”
Antigen-Antibody Interaction : Avidity
"Sum of all attractive forces btwn an AG and AB ex IgG (2 sites) has less avidity than IgM (10 sites) "
Valency of interaction vs Avidity of interaction
“Valency connected to places/options to connect to AGs (connection sites)
Avidity increased by valency (due to increased connection sites) “
types of bonds in Antigen-antibody interaction
“Multiple, reversible, intermolecular attractions btwn AG and amino acids @ binding site
Noncovalent bonds -> binding forces (electrostatic, hydrogen bonds, van der waals, hydrophobic forces) “
Electrostatic forces
“Type of Noncovalent bond
- attraction btwn opposite charges”
Hydrogen bonds
“Type of Noncovalent bond
- Hydrogen shared btwn electronegative atoms (N,O)”
Van der Waals forces
“Type of Noncovalent bond
- Fluctuations in electron clouds around molecules oppositely polarize neighboring atoms “
Hydrophobic forces
“Type of Noncovalent bond
- Hydrophobic groups interact unfavorably w/ water and tend to pack together to exclude water molecules. The attraction also involves van der Waals forces “
Goodness of fit
Strongest bonding develops when antigens and antibodies are close to eachother and when shapes of the antigenic determinants and antigen-binding sites conform to each other
Immune cells with Fc receptors are
”"”Professional phagocytes”” - Macs, Neutrophils, dendritic cells, mast cells
* other cells - eosinophils, B cells, NK cells, Platelets “
Immune cells with FcE receptors are
Mast cells ~ bind IgE and induce allergic reactions
Immune cells with FcY receptors are
“**not sure which cells bind?
- receptors are most important in opsonization of particles bound by IgG”
ITAM activation
“Immunoreceptor tyrosin-based activation motif
- induces various signals to promote activation of phagocytosis/cell mediated cytotoxicity “
ITIM binding leads to
“Immunoreceptor tyrosin-based Inhibitory motif
- creates an inhibitory action; ““negative signaling”” helps to regulate cell activation “
