Antibodies 3/3

Question 1

Major function/property of which Ig is Opsonization

Answer 1

IgG1,3

Question 2

Major function/property of which Ig is Sensitization for killing NK cells

Answer 2

IgG1,3

Question 3

Major function/property of which Ig is Sensitization of mast cells

Answer 3

IgE

Question 4

Major function/property of which Ig is Sensitization of basophils

Answer 4

IgD,IgE

Question 5

Major function/property of which Ig is Activation of complement system

Answer 5

IgM,IgG3,1

Question 6

Major function/property of which Ig is transport across epithelium

Answer 6

IgA (dimer)

Question 7

Major function/property of which Ig is Transport across placenta

Answer 7

IgG1,3,4

Question 8

Major function/property of which Ig is Diffusion into extravascular sites

Answer 8

IgG1,2,3,4,IgA (monomer)

Question 9

Antigen-Antibody Interaction : Affinity

Answer 9

“initial force of attraction that exists between a single Fab site on an AB molecule and a single epitope on the AG
- allows ABs to stick to AG”

Question 10

Antigen-Antibody Interaction : Avidity

Answer 10

"Sum of all attractive forces btwn an AG and AB 
ex IgG (2 sites) has less avidity than IgM (10 sites) "

Question 11

Valency of interaction vs Avidity of interaction

Answer 11

“Valency connected to places/options to connect to AGs (connection sites)
Avidity increased by valency (due to increased connection sites) “

Question 12

types of bonds in Antigen-antibody interaction

Answer 12

“Multiple, reversible, intermolecular attractions btwn AG and amino acids @ binding site
Noncovalent bonds -> binding forces (electrostatic, hydrogen bonds, van der waals, hydrophobic forces) “

Question 13

Electrostatic forces

Answer 13

“Type of Noncovalent bond

- attraction btwn opposite charges”

Question 14

Hydrogen bonds

Answer 14

“Type of Noncovalent bond

- Hydrogen shared btwn electronegative atoms (N,O)”

Question 15

Van der Waals forces

Answer 15

“Type of Noncovalent bond

- Fluctuations in electron clouds around molecules oppositely polarize neighboring atoms “

Question 16

Hydrophobic forces

Answer 16

“Type of Noncovalent bond
- Hydrophobic groups interact unfavorably w/ water and tend to pack together to exclude water molecules. The attraction also involves van der Waals forces “

Question 17

Goodness of fit

Answer 17

Strongest bonding develops when antigens and antibodies are close to eachother and when shapes of the antigenic determinants and antigen-binding sites conform to each other

Question 18

Immune cells with Fc receptors are

Answer 18

”"”Professional phagocytes”” - Macs, Neutrophils, dendritic cells, mast cells
* other cells - eosinophils, B cells, NK cells, Platelets “

Question 19

Immune cells with FcE receptors are

Answer 19

Mast cells ~ bind IgE and induce allergic reactions

Question 20

Immune cells with FcY receptors are

Answer 20

“**not sure which cells bind?

- receptors are most important in opsonization of particles bound by IgG”

Question 21

ITAM activation

Answer 21

“Immunoreceptor tyrosin-based activation motif

- induces various signals to promote activation of phagocytosis/cell mediated cytotoxicity “

Question 22

ITIM binding leads to

Answer 22

“Immunoreceptor tyrosin-based Inhibitory motif

- creates an inhibitory action; ““negative signaling”” helps to regulate cell activation “