Antibiotics

Question 1

What is steptomycin’s function ?

Does it target prokaryotes or eukaryotes?

Answer 1

Binds 30s subunit interfering with the binding of fmet -> complex not formed and initiation is impaired

Prokaryotes

Question 2

What are cindamycin and erthomycin’s function ?

Does it target prokaryotes or eukaryotes?

Answer 2

Binds to 50s blocking translocation of ribosome so it can’t move down the chain —> initiation blocked

Prokaryotes

Question 3

What is tetracylcine’s function ?

Does it target prokaryotes or eukaryotes?

Answer 3

Binds to 30s and blocks entry of aminoacyl tRNAto ribosomal complex —> elongation impaired

Prokaryotes

Question 4

What is chloramphenciol’s function ?

Does it target prokaryotes or eukaryotes?

Answer 4

Inhibits peptidyl transferase activity and peptide bond formation —> no polypeptide

Prokaryotes

Question 5

What are shiva toxin and ricin’s function ?

Does it target prokaryotes or eukaryotes?

Answer 5

Bind to large 60s subunit blocking entry of aminylacyl tRNA

Eukaryotes

Question 6

What is diphtheria toxin’s function ?

Does it target prokaryotes or eukaryotes?

Answer 6

Inactivates GTP bound EF-2 interfering with translocation

Eukaryotes

Question 7

What is cyclohexamide’s function ?

Does it target prokaryotes or eukaryotes?

Answer 7

Inhibits peptidyl transferase and impair peptide bond formation

Eukaryotes

Question 8

What is puromycin’s function ?

Does it target prokaryotes or eukaryotes?

Answer 8

Elongation inhibitor —> premature termination
Resembles 3’ end of amino acylated tRNA, enters A site and adds to chain —> pormyclated chain and termination

Both pro and eukaryotes