Antibiotics

Question 1

Small ribosomal antibiotics?

Answer 1

Tetracycline

Streptomycine

Question 2

Mechanism of Tetracycline?

Answer 2

Block A-site

Question 3

Mechanism(s) of Streptomycine?

Answer 3

1) Premature STOP

2) A —X–> P

Question 4

Large ribosomal antibiotics?

Answer 4

Oxazolidinones

Chloramphenicol

Macrolides

Erythromycin

Question 5

Mechanism of Oxazolidinones?

Answer 5

Block association of large sub

Question 6

Mechanism of Chloramphenicol?

Answer 6

Block peptide transferase centre (peptide can’t form)

Question 7

Mechanism of macrolides?

Answer 7

Block exit tunnel (peptide can’t leave)

Question 8

Mechanism of Erythromycin?

Answer 8

A —X–> P

Question 9

Paths of resistance?

Answer 9

Eflux pump (remove anti from cell)

Enzymatic degradation of anti

Chem modification of anti by enzymes (change shape of target, small peptides prevent bonding)

Mutate themselves

Question 10

Chloramphenicol resistance?

Answer 10

Alters PTC

Methylation of rRNA (anti can’t bind) (Ermc methyltransferase)

Question 11

Erythromycin resistance?

Answer 11

Mutation of rRNA at tunnel

Ermc methyltransferase

Question 12

Three ways bacteria share resistance?

Answer 12

Transformation– dead bacteria

Conjugation – plasmids

Transduction – via viruses

Question 13

Andrew MacArthur?

Answer 13

Antiomicrobrial drug resistance and molec epidemiology

Large database of resistance

Question 14

Dr. Marie Elliot?

Answer 14

Develops antibiotics