Antibiotics Flashcards
What is the structure of penicillin?
- Thiazolidine ring
- B-lactam ring
What is the mechanism of action of B-lactams?
- interact with enzymes localized in cell membrane whose action is to build the cell wall
- prevents peptidoglycan synthesis (Gm +)
What is the dose of antibiotics limited by?
Toxicity
What is the minimum inhibitory concentration (MIC) of an antibiotic?
the lowest concentration of antibiotic that, under certain in vitro test conditions, inhibits further bacterial growth
What is the minimum bactericidal concentration (MBC) of an antibiotic?
lowest concentration that kills bacteria
In which diseases is the bactericidal effect thought to be important (relative to bacteriostatic effect)?
- endocarditis (only bacterial infection for which bacterial effect is absolutely necessary for a cure)
- meningitis or osteomylitis
- neutropenia
What is Penicillin G?
- benzyl penicillin
- first form of penicillin used in humans
- aqueous
- most destroyed by gastric acid, so given IV
What is penicillin V?
- add a phenoxy group to side chain of penicillin G
- can be given orally b/c it resists acid
What does probenecid do?
- delays excretion of penicillin at renal tubules
- Musher has never used it, doesn’t think its necessary
Where does penicillin go in the body (distribution)?
-distributes to EFC (22% of lean body weight)
How rapidly is penicillin excreted (clearance)?
T1/2 = 20 minutes
-excreted via kidney
By percentage of body weight, how much ECF do we have?
22% (15 liters)
Why does penicillin only distribute to ECF?
- cell membranes exclude it
- polar molecule
What conditions are associated with greatly increased ECF?
- Heart failure
- cirrhosis w/ ascites
- renal failure *doses of abx need to be adjusted to reflect this
How is penicillin excreted?
100% Kidney
*keep creatinine clearance and kidney failure in mind
Why is the peak concentration of penicillin much lower than expected?
- half life of 20 minutes
- infusion takes 20-30 minutes
How should penicillin be administered? Why?
- IV at close intervals, usually 4hrs
- Keeps concentration above MIC about 2/3 of the time
if you double the dose of IV penicillin, you only add ___________ of coverage
20 min (= one half-life)
So, if you want to increase the concentration of penicillin, how do you do it?
- shorten the dosing interval
- doubling dose will only add 20 min of coverage
How is nafcillin excreted?
-Kidney AND liver
What are the pharmakinetic advantages and disadvantages of amoxicillin over penicillin?
- lower peak concentration
- level above MIC may be more sustained because drug is being absorbed in GI tract as its being excreted
- nearly 100% is absorbed in GI tract
What are procaine and benzathine penicillin designed for?
-slow absorption from injection site
What are the three factors that determine the outcome of treatment with antibiotics?
1) time that tissue levels exceed MIC
2) peak level of concentration (sometimes binding is irreversible above certain concentration)
3) area under the curve above MIC. (ex. both duration and height of level of quinolones)
Beta-lactams. Must exceed MIC for ______ of treatment period.
>60%
Antibiotic resistance may arise by __________ or by acquisition of ___________.
- point mutation
- genetic material
What are the general mechanisms of bacterial resistance to antibiotics?
1) make an enzyme that disrupts Abx at active site.
2) alter site at which abx act
3) generate new pathway that bypasses the one blocked by abx (MRSA)
4) alter entrance of antibiotic into bacterium (porins)
5) increase rate with which abx is pumped out of the bacterium
Resistance of pneumococci to beta-lactam antibiotics is of concern in ______________, not in pneumonia, sinusitis or otitis media
meningitis (antibiotic passage across blood-brain barrier)
What are bacterial porins?
- Porins are protein channels of outer membrane
- Transport polar molecules into periplasmic space; from there, diffuse through cell wall into cytoplasm
- Mechanism only for gram negative rods; Gram positive bacteria do not have them
Which -cillins are resistant to B-lactamase?
- Methicillin
- Nafcillin
- oxacillin
What drug is usually used against methicillin-susceptible S. aureus?
Nafcillin
Which drugs is MRSA resistant to?
- all penicilins
- cephalosporins (except ceftaroline)
- carbapenems
What is the half life of Amoxicillin?
45-60 minutes
What types of organisms is ampicillin good against?
Gm (-) organisms
What is piperacillin used against?
- Gm (-) rods
- pseudomonas
**widely used in TMC, especially with B-lactamase inhibitor (tazobactam)
What are the B-lactamase inhibitors?
- clavulanic acid
- sulbactam
- tazobactam
What is Augmentin made of?
-Amoxicillin + Clavulanic acid (b-lactamase blocker)
Can Augmentin be used against MRSA?
no
What is Unasyn?
-Ampicillin + sulbactam
What is Zosyn?
Piperacililn + tazobactam
What are the adverse effects of penicillin?
- skin rash (10%)
- anaphylaxis (0.01%)
What is the structure of cephalospornis?
- four membered ring attached to a six membered ring
- side chain on four-membered ring
What are the first generation cephalosporins?
- Cefazolin
- Cephalexin (oral cefazolin)
What is Cefazolin used against?
- non-MRSA Staph aureus
- Group A strep, & other soft tissue infections
- Gm (-) rods
**no effect against enterococcus
What is antibiotic prophylaxis for surgery?
-give abx before surgery so that it is in the tissues at the time of surgery
What is cephalexin?
-oral version of cefazolin
What are the third generation of cephalosporins?
- cefotaxime (Claforan)
- ceftriaxone (Rocephin)
- cefpodoxime (Vantin) = oral
- ceftazidime (pseudo)
What are third generation cephalosporins effective against?
- Strep
- pneumo
- Haemophilus
- Moraxella
- Gonococci
- Lyme disease
- Gram negative rods (except Pseudomonas)
What is the half life of Ceftriaxone?
6-8 hours
*can be given every 24 hours
What cephalosporins are good against pseudomonas?
- Ceftazidime (3G)
- Cefepime (4G)
- ceftaroline (5G) (MRSA)
Does penicillin cross the blood brain barrier?
Not very well
Inflammation decreases the rate at which the choroid plexus pump removes _________ from CSF, so, during meningitis, antibiotic levels persist whereas in the absence of meningitis they are rapidly cleared.
antibiotics
Does ceftriaxone cross the blood-brain barrier?
- yes
- good for meningitis
What is Cefpodoxime?
-Oral 3rd generation cephalosporin
What are the fourth generation cephalosporins?
-cefepime
When is cefepime used?
-like ceftriaxone, but with a good effect against pseudomonas
*really only used for pseudomonas
What is ceftaroline?
- “fifth generation” cephalosporin
- has all properties of cefepime, but ALSO active against MRSA